Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,024	C→T	R761H (CGC→CAC)	lacZ ←	beta‑D‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,024	0	C	T	94.4%	280.5 / 3.4	89	R761H (CGC→CAC)	lacZ	beta‑D‑galactosidase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (38/46);  total (40/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

GTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCG  >  NC_000913/363927‑364117                                                                                                  |                                                                                              gTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACtggtgg                                                                                             <  2:164336/100‑1 (MQ=255) gTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACtggtgg                                                                                             <  1:322228/100‑1 (MQ=255)   cGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGtt                                                                                           <  2:162388/100‑1 (MQ=255)     ttATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTaa                                                                                         <  2:337063/100‑1 (MQ=255)     ttATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTaa                                                                                         <  1:702734/100‑1 (MQ=255)      tATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTaaa                                                                                        >  2:521226/1‑100 (MQ=255)      tATCCAGCGGTGACCGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTCGTTTTCTAACGCAAATCCACATCTGTGAAAGAAAGCCTGTCTAGTGGTTaaa                                                                                        >  2:1937112/1‑100 (MQ=255)        tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                      <  2:571196/100‑1 (MQ=255)         ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGCAAGAAAGCCTGACTGGTGGTTAGATTg                                                                                     >  1:1573098/1‑100 (MQ=255)         ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTg                                                                                     >  1:1294315/1‑100 (MQ=255)         ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTg                                                                                     <  2:109262/100‑1 (MQ=255)          cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTg                                                                                     <  1:934729/99‑1 (MQ=255)          cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                    <  1:390606/100‑1 (MQ=255)          cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                    >  1:1841624/1‑100 (MQ=255)            gCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCa                                                                                  <  2:1633841/100‑1 (MQ=255)            gCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCa                                                                                  <  1:1217408/100‑1 (MQ=255)               gTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACg                                                                               >  1:1374045/1‑100 (MQ=255)               gTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACg                                                                               <  2:456104/100‑1 (MQ=255)                 gCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCt                                                                             >  1:1796900/1‑100 (MQ=255)                  cACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCGCATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGc                                                                              >  1:317247/1‑98 (MQ=255)                  cACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCtt                                                                            <  2:1204903/100‑1 (MQ=255)                    cGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttat                                                                          >  2:1413716/1‑100 (MQ=255)                     gggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatt                                                                         >  1:1931160/1‑100 (MQ=255)                     gggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatt                                                                         >  2:1012739/1‑100 (MQ=255)                      ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                        <  2:1772541/100‑1 (MQ=255)                      ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                        >  2:428138/1‑100 (MQ=255)                       gTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                        >  2:80425/1‑99 (MQ=255)                       gTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAc                                                                       <  2:1742452/100‑1 (MQ=255)                        tGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAcc                                                                      <  1:842276/100‑1 (MQ=255)                        tGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAcc                                                                      >  2:1897972/1‑100 (MQ=255)                        tGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAcc                                                                      >  2:826158/1‑100 (MQ=255)                         gAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAccc                                                                     <  1:1034245/100‑1 (MQ=255)                          aaCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCa                                                                    >  2:211763/1‑100 (MQ=255)                           aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAg                                                                   <  1:1543046/100‑1 (MQ=255)                             tGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCt                                                                 >  1:1600544/1‑100 (MQ=255)                              gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                <  1:25092/100‑1 (MQ=255)                              gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                >  1:177061/1‑100 (MQ=255)                              gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                <  2:991561/100‑1 (MQ=255)                                tCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGa                                                              <  2:1178029/100‑1 (MQ=255)                                 cgcgCAGCGGCGTCAGCAGTTGTTTTTTATCGCTAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGAt                                                             <  2:46666/100‑1 (MQ=255)                                  gcgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGAtn                                                            <  1:1880723/100‑2 (MQ=255)                                  gcgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                            >  1:318441/1‑100 (MQ=255)                                   cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                           <  2:957563/100‑1 (MQ=255)                                     cAGCGGCGTCAGCAGTTGTTTTTTATCGCGAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTTAATTGCCAACGCTTATTACCCAGCTCGATGCaa                                                         <  1:1173279/100‑1 (MQ=255)                                       gcggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaaaa                                                       >  1:147800/1‑100 (MQ=255)                                          gcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATc                                                    <  2:776211/100‑1 (MQ=255)                                          gcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATc                                                    <  2:442911/100‑1 (MQ=255)                                           cgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATcc                                                   <  2:965179/100‑1 (MQ=255)                                             tCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAt                                                 <  2:1563401/100‑1 (MQ=255)                                              cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAtt                                                <  2:799811/100‑1 (MQ=255)                                              cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAtt                                                >  1:1484222/1‑100 (MQ=255)                                                gcagTTTTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                              <  1:332930/100‑1 (MQ=255)                                                gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGTTTATTACCCAGCTCGATGCAAAAATCCATTTc                                              <  2:1841636/100‑1 (MQ=255)                                                gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                              >  2:1082875/1‑100 (MQ=255)                                                   gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                           <  2:1307251/100‑1 (MQ=255)                                                     tgttTTTTATTGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTACTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                         <  1:1274323/100‑1 (MQ=255)                                                     tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                         >  2:1274328/1‑100 (MQ=255)                                                     tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                         <  2:689586/100‑1 (MQ=255)                                                       ttttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtg                                       >  2:283605/1‑100 (MQ=255)                                                        tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                      <  2:177062/100‑1 (MQ=255)                                                          tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                    <  1:810227/100‑1 (MQ=255)                                                          tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                    <  1:1665234/100‑1 (MQ=255)                                                           ttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCa                                   <  1:957242/100‑1 (MQ=255)                                                               cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                               <  2:1324623/100‑1 (MQ=255)                                                                gttatgccACATCTCTGAAAGAAATCCTTACTTTCGGTTAAATGGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                              <  2:1330047/94‑1 (MQ=255)                                                                gacAATCCACATCCGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                              >  2:1308836/3‑100 (MQ=255)                                                                gCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                              >  2:1009318/1‑100 (MQ=255)                                                                 ccAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCg                             <  1:1897959/100‑1 (MQ=255)                                                                   aaTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTCCCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                           <  2:362504/100‑1 (MQ=255)                                                                    aTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGa                          <  1:1128807/100‑1 (MQ=255)                                                                      ccACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATg                        >  1:675359/1‑100 (MQ=255)                                                                           tCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                   <  2:456068/100‑1 (MQ=255)                                                                           tCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGATTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCg                     >  1:799007/1‑98 (MQ=255)                                                                            cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                  >  2:532483/1‑100 (MQ=255)                                                                             tgtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                 <  1:283603/100‑1 (MQ=255)                                                                             tgtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                 >  2:117472/1‑100 (MQ=255)                                                                             tgtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                 >  2:1528714/1‑100 (MQ=255)                                                                             tgtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                 >  1:190266/1‑100 (MQ=255)                                                                              gtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                >  2:1250173/1‑100 (MQ=255)                                                                                gaaagaaaGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg              >  1:188680/1‑100 (MQ=255)                                                                                 aaagaaagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc             >  2:700619/1‑100 (MQ=255)                                                                                   agaaagGCGGACGGGTGGGTAAAGTGCCAACGGAAATTACCAAGCTGGATGCAAAAATCCATATCGCTGGTGGTCAGATGCGGGAAGGCGTGGTACgcgg           >  1:1911122/1‑100 (MQ=255)                                                                                    gaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAGAAATCCATTGCGCTGATGGTCAGAGGCGGGATGGCGTGGGACgcggc          >  1:1312789/1‑100 (MQ=255)                                                                                      aagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGcggcgg        <  2:1374053/100‑1 (MQ=255)                                                                                       agCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCggg       <  2:1796911/100‑1 (MQ=255)                                                                                        gCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg      <  1:1713735/100‑1 (MQ=255)                                                                                          cTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAg    <  2:205155/100‑1 (MQ=255)                                                                                          cTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAg    >  1:118389/1‑100 (MQ=255)                                                                                           tGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGc   >  2:1106326/1‑100 (MQ=255)                                                                                           tGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGc   >  1:798202/1‑100 (MQ=255)                                                                                            gACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATGTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg  <  1:1082874/100‑1 (MQ=255)                                                                                                  |                                                                                              GTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCG  >  NC_000913/363927‑364117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑