Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	367,830	G→A	Y197Y (TAC→TAT)	mhpR ←	mhp operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	367,830	0	G	A	92.5%	282.4 / 9.1	93	Y197Y (TAC→TAT)	mhpR	mhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base A (46/40);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTC  >  NC_000913/367731‑367925                                                                                                    |                                                                                                cacCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCg                                                                                                 >  2:1392816/1‑100 (MQ=255)   ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATa                                                                                               <  2:1624302/100‑1 (MQ=255)          cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCctct                                                                                        >  1:278004/1‑100 (MQ=255)          cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCctct                                                                                        >  2:1676037/1‑100 (MQ=255)           aGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCtt                                                                                       >  1:920567/1‑100 (MQ=255)            gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCTATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCttt                                                                                      >  1:285999/1‑100 (MQ=255)              cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgc                                                                                    >  1:505945/1‑100 (MQ=255)                   aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcg                                                                               <  2:753425/100‑1 (MQ=255)                   aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcg                                                                               >  2:347941/1‑100 (MQ=255)                    aTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgc                                                                              >  2:1377721/1‑100 (MQ=255)                        cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcgc                                                                          >  2:838442/1‑100 (MQ=255)                         ccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGcc                                                                         >  1:985877/1‑100 (MQ=255)                          cGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCa                                                                        >  2:370901/1‑100 (MQ=255)                            ttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGa                                                                      <  1:1484282/100‑1 (MQ=255)                              gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAAt                                                                    >  1:164608/1‑100 (MQ=255)                               ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                   >  1:1896237/1‑100 (MQ=255)                               ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                   >  1:1895938/1‑100 (MQ=255)                               ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                   >  2:8413/1‑100 (MQ=255)                                tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTCATCCAAGCCGCGGAAGTTCTGTCGATAACCCTCTTTGCGCGCGCGCGCCAGACTgg                                                                  >  2:433245/1‑100 (MQ=255)                                 cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  2:963006/1‑100 (MQ=255)                                 cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  2:444074/1‑100 (MQ=255)                                    tGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTc                                                              >  1:859028/1‑100 (MQ=255)                                     gcgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTcc                                                             <  1:1250295/100‑1 (MQ=255)                                     gcgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTcc                                                             <  2:319793/100‑1 (MQ=255)                                     gcgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTcc                                                             >  2:806814/1‑100 (MQ=255)                                       gcAGCGGTACGGCGATAGAGGCGCTCTTCGCCTCCTGAGCCCAGCCGCGGTAGTTCTGTGCATAACCCTCTTTGCGCGCGCGCGCCATAATGGCTTcc                                                             <  1:375861/98‑1 (MQ=255)                                       gcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAg                                                           <  2:907406/100‑1 (MQ=255)                                        cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGc                                                          >  1:1320147/1‑100 (MQ=255)                                         aGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCt                                                         <  2:743909/100‑1 (MQ=255)                                              tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    <  2:252068/100‑1 (MQ=255)                                              tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    >  1:332932/1‑100 (MQ=255)                                              tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    <  2:345121/100‑1 (MQ=255)                                                cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                  >  2:519327/1‑100 (MQ=255)                                                cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                  >  1:827772/1‑100 (MQ=255)                                                   cGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTc                                               >  1:387259/1‑100 (MQ=255)                                                    gATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTcc                                              <  2:1482255/100‑1 (MQ=255)                                                      tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCg                                            <  2:783004/100‑1 (MQ=255)                                                      tAGAGGCGATCTTCTCCTCCTGATCCCAGCCACGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCg                                            <  2:763032/100‑1 (MQ=255)                                                       agagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTccctt                                           >  1:1184137/1‑98 (MQ=255)                                                        gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          <  1:963005/100‑1 (MQ=255)                                                        gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          >  2:67453/1‑100 (MQ=255)                                                         agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  2:1744076/1‑100 (MQ=255)                                                         agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  2:1181522/1‑100 (MQ=255)                                                         agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  2:334877/1‑100 (MQ=255)                                                         agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  2:334704/1‑100 (MQ=255)                                                          ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        >  2:1570858/1‑100 (MQ=255)                                                          ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        <  2:332934/100‑1 (MQ=255)                                                          ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGCCTTCCAGCTTTAACGGTTCCCGTGcc                                        >  1:916041/1‑100 (MQ=255)                                                            cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATACCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                      <  2:1438811/100‑1 (MQ=255)                                                            cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                      <  2:1437903/100‑1 (MQ=255)                                                            cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCAAg                                      >  1:329210/1‑100 (MQ=255)                                                             gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                     >  1:1574654/1‑100 (MQ=255)                                                              aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                    <  1:429065/100‑1 (MQ=255)                                                                  tctaCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCGTTAACGGTTCCCGTGCCAGTTGATa                                >  2:939441/1‑100 (MQ=255)                                                                    tcctccTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGt                              >  2:1409856/1‑100 (MQ=255)                                                                      ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtca                            <  1:1744065/100‑1 (MQ=255)                                                                      ctcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtca                            >  1:1472975/1‑100 (MQ=255)                                                                       tcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcat                           <  1:1848471/100‑1 (MQ=255)                                                                       tcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcat                           <  1:1676027/100‑1 (MQ=255)                                                                        cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          >  1:127022/1‑100 (MQ=255)                                                                        cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          >  2:223948/1‑100 (MQ=255)                                                                         ctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                         >  2:1613020/1‑100 (MQ=255)                                                                          tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                         >  1:312655/1‑99 (MQ=255)                                                                          tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                        >  2:1465531/1‑100 (MQ=255)                                                                          tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                        <  2:278006/100‑1 (MQ=255)                                                                          tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                        >  2:1332742/1‑100 (MQ=255)                                                                          tGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGCTGATAGTCATCAc                        <  2:1320154/100‑1 (MQ=255)                                                                               ccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                   >  2:1230931/1‑100 (MQ=255)                                                                                 aGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                 <  2:1378929/100‑1 (MQ=255)                                                                                 aGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                 >  1:1036406/1‑100 (MQ=255)                                                                                 aGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                 <  1:546333/100‑1 (MQ=255)                                                                                 aGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                 <  2:340876/100‑1 (MQ=255)                                                                                 aGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCg                 <  2:341156/100‑1 (MQ=255)                                                                                  gCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCgg                <  2:1067278/100‑1 (MQ=255)                                                                                   ccGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg               <  1:1381499/100‑1 (MQ=255)                                                                                   ccGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg               <  2:965373/100‑1 (MQ=255)                                                                                   ccGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg               <  1:765275/100‑1 (MQ=255)                                                                                    cgcgGTAGTTCTGTCCATACCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa              <  2:437227/100‑1 (MQ=255)                                                                                    cgcgGTAGTTCTGTCCATAACCCTCTTTGCGCGAGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa              <  2:286001/100‑1 (MQ=255)                                                                                     gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             <  2:1004456/100‑1 (MQ=255)                                                                                     gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             <  1:806813/100‑1 (MQ=255)                                                                                     gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             <  2:859029/100‑1 (MQ=255)                                                                                     gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             <  2:838078/100‑1 (MQ=255)                                                                                     gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg             >  1:1464739/1‑100 (MQ=255)                                                                                      cgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAgg            >  2:1890765/1‑100 (MQ=255)                                                                                        gTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt          <  2:1508108/100‑1 (MQ=255)                                                                                           gTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc       >  1:1478549/1‑100 (MQ=255)                                                                                           gTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc       <  1:347939/100‑1 (MQ=255)                                                                                             tCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAt     >  1:1247284/1‑100 (MQ=255)                                                                                                gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  <  1:519325/100‑1 (MQ=255)                                                                                                gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  <  1:1377713/100‑1 (MQ=255)                                                                                                gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  <  1:736746/100‑1 (MQ=255)                                                                                                gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  <  2:646692/100‑1 (MQ=255)                                                                                                gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  >  1:1846065/1‑100 (MQ=255)                                                                                                    |                                                                                                CACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTC  >  NC_000913/367731‑367925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑