Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 383,477 G→A G153G (GGC→GGT)  yaiP ← putative family 2 glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913383,4770GA92.7% 203.0 / 4.3 68G153G (GGC→GGT) yaiPputative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (37/26);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

TAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAA  >  NC_000913/383380‑383571
                                                                                                 |                                                                                              
taataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCAcc                                                                                              <  2:1371640/100‑1 (MQ=255)
 aataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCt                                                                                             >  1:119160/1‑100 (MQ=255)
  ataaACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCtt                                                                                            >  2:312155/1‑100 (MQ=255)
    aaaCGGTGCGCCACCCAGGAGCTGCTGTAGTGTTCGCTTCATAACAATCATCGTCAACTTTATGGTCGCGCGGATGTGCGGTCACAGACCCGCAcc                                                                                              <  2:1316799/96‑1 (MQ=255)
    aaaCGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTc                                                                                          <  2:53668/100‑1 (MQ=255)
     aaCGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCa                                                                                         <  1:1334207/100‑1 (MQ=255)
     aaCGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCa                                                                                         >  2:1707325/1‑100 (MQ=255)
      aCGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCaa                                                                                        <  1:1305342/100‑1 (MQ=255)
        ggTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACg                                                                                      <  2:1908807/100‑1 (MQ=255)
            cgcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGt                                                                                  <  2:730106/100‑1 (MQ=255)
            cgcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGt                                                                                  >  2:935380/1‑100 (MQ=255)
            cgcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGt                                                                                  <  2:277332/100‑1 (MQ=255)
             gcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTa                                                                                 >  2:975191/1‑100 (MQ=255)
             gcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTa                                                                                 >  2:1123175/1‑100 (MQ=255)
             gcCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTa                                                                                 >  1:541475/1‑100 (MQ=255)
              ccACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTag                                                                                <  2:1002950/100‑1 (MQ=255)
              ccACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTag                                                                                >  1:63639/1‑100 (MQ=255)
                 cccAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAgg                                                                             <  2:512185/100‑1 (MQ=255)
                 cccAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAgg                                                                             >  2:682162/1‑100 (MQ=255)
                   cAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGa                                                                           >  2:139947/1‑100 (MQ=255)
                      gAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATg                                                                        <  2:586435/100‑1 (MQ=255)
                        gctgctGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgcc                                                                      <  1:726102/100‑1 (MQ=255)
                         ctgctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgccg                                                                     <  2:1731013/100‑1 (MQ=255)
                          tgctgTAGCGTGCGCTTCATAACAATCACCGGCAACTCTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATgccgc                                                                    >  1:1047216/1‑100 (MQ=255)
                            ctgTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCt                                                                  >  2:1164507/1‑100 (MQ=255)
                              gTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTAc                                                                >  2:321155/1‑100 (MQ=255)
                                    tGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATc                                                          <  2:187571/100‑1 (MQ=255)
                                    tGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATc                                                          <  2:973129/100‑1 (MQ=255)
                                     gcgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCg                                                         >  1:911979/1‑100 (MQ=255)
                                      cgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCgg                                                        <  1:150506/100‑1 (MQ=255)
                                      cgcTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCgg                                                        >  2:1675234/1‑100 (MQ=255)
                                         ttCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCAc                                                     <  2:1340801/100‑1 (MQ=255)
                                          tCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCAcc                                                    >  2:196505/1‑100 (MQ=255)
                                           cATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACcg                                                   >  1:334299/1‑100 (MQ=255)
                                               aCAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCt                                               <  1:637302/100‑1 (MQ=255)
                                                cAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTc                                              <  2:279084/100‑1 (MQ=255)
                                                cAATCATCGGCAACTTTACGGTCGCGCGGATGAGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCt                                               >  2:1844922/1‑99 (MQ=255)
                                                     atcGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAAttt                                         <  1:312153/100‑1 (MQ=255)
                                                      tcGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTc                                        >  1:1392000/1‑100 (MQ=255)
                                                       cGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCt                                       >  2:30807/1‑100 (MQ=255)
                                                            aCTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAg                                  <  2:151560/100‑1 (MQ=255)
                                                                 aCGGTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCatat                             >  1:614546/1‑100 (MQ=255)
                                                                    gTCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGc                          >  1:296056/1‑100 (MQ=255)
                                                                     tCGCGCGGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGcc                         <  2:178252/100‑1 (MQ=255)
                                                                      cgcgcgGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGccc                        <  2:961825/100‑1 (MQ=255)
                                                                       gcgcgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGATCAATTTCTGCCAGCATATAGccc                        >  1:1743877/1‑99 (MQ=255)
                                                                        cgcgGATGTGCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCAt                      >  1:562731/1‑100 (MQ=255)
                                                                              tgtgCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTc                >  1:424393/1‑100 (MQ=255)
                                                                              tgtgCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTc                <  2:297476/100‑1 (MQ=255)
                                                                               gtgCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCt               >  2:255397/1‑100 (MQ=255)
                                                                                tgCGGTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCtt              >  2:526372/1‑100 (MQ=255)
                                                                                 gCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTg             >  1:1605386/1‑100 (MQ=255)
                                                                                    gTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATc          >  2:310963/1‑100 (MQ=255)
                                                                                    gTAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATc          <  1:718695/100‑1 (MQ=255)
                                                                                     tAACAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCg         <  1:196504/100‑1 (MQ=255)
                                                                                       aCAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCggg       >  1:786367/1‑100 (MQ=255)
                                                                                       aCAGACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCggg       >  1:1606600/1‑100 (MQ=255)
                                                                                          gACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCgg    >  1:1096772/1‑100 (MQ=255)
                                                                                          gACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCgg    <  2:733025/100‑1 (MQ=255)
                                                                                          gACCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCgg    >  2:1869510/1‑100 (MQ=255)
                                                                                           aCCCGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGa   >  1:459280/1‑100 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATATCCCATTCCGTCTTGATCGGGCGGaa  >  2:1820432/1‑100 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa  <  1:846129/100‑1 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa  >  2:794707/1‑100 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa  >  1:983121/1‑100 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa  <  1:30807/100‑1 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa  >  2:1694198/1‑100 (MQ=255)
                                                                                            cccGCACCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGaa  >  1:1790883/1‑100 (MQ=255)
                                                                                                 |                                                                                              
TAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACGGTCGCGCGGATGTGCGGTAACAGACCCGCGCCTTTCAACGCAGTAGAGGGAATGCCGCCTACGGCATCGGCACCGCGCTCAATTTCTGCCAGCATATAGCCCATTCCGTCTTGATCGGGCGGAA  >  NC_000913/383380‑383571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑