| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 459,256 | T→C | D123D (GAT→GAC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 459,256 | 0 | T | C | 92.7% | 213.0 / 5.3 | 68 | D123D (GAT→GAC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (25/38); total (27/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCG > NC_000913/459158‑459352 | cTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATtgatg > 1:442601/1‑100 (MQ=255) ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATTGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg > 1:1078713/1‑100 (MQ=255) ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGTGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg > 1:296582/1‑100 (MQ=255) gTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGc < 2:820988/100‑1 (MQ=255) cGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGGCGCCGACCATTGACGAGCgg > 1:1708824/1‑100 (MQ=255) gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCggg < 1:287319/100‑1 (MQ=255) gAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCggg < 1:1254650/100‑1 (MQ=255) aGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGa < 2:886822/100‑1 (MQ=255) aGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGa < 2:8696/100‑1 (MQ=255) gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAg > 1:131506/1‑100 (MQ=255) ttACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAgg < 1:1295791/100‑1 (MQ=255) tACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGTGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAgg > 1:1362604/1‑99 (MQ=255) tACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGa < 1:422536/100‑1 (MQ=255) cAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAg < 2:1512427/100‑1 (MQ=255) aGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGt < 1:1500647/100‑1 (MQ=255) cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGc > 1:451280/1‑100 (MQ=255) cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGc < 1:1260665/100‑1 (MQ=255) cgcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGc < 1:1006319/100‑1 (MQ=255) gcgcgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCt < 2:1523150/100‑1 (MQ=255) cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGt > 1:1397211/1‑100 (MQ=255) gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg < 2:1650159/100‑1 (MQ=255) gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg < 2:1723075/100‑1 (MQ=255) gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg < 2:920353/100‑1 (MQ=255) gcgTATTTCTGCGCTCTCTGACAACGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg < 2:748276/100‑1 (MQ=255) cgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGc > 1:1739786/1‑100 (MQ=255) cgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGc < 1:336454/100‑1 (MQ=255) gTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCg < 2:637096/100‑1 (MQ=255) aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTa > 1:712877/1‑100 (MQ=255) aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTa < 1:650558/100‑1 (MQ=255) ttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACt > 1:707741/1‑100 (MQ=255) tCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTg > 1:434431/1‑100 (MQ=255) cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc < 1:1781972/100‑1 (MQ=255) cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGc < 2:210223/100‑1 (MQ=255) gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa < 2:451282/100‑1 (MQ=255) gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa < 2:558169/100‑1 (MQ=255) cgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAAt > 2:1402039/1‑100 (MQ=255) ctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGc < 2:1036406/100‑1 (MQ=255) tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc < 1:908957/100‑1 (MQ=255) tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc < 1:1419301/100‑1 (MQ=255) tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc < 2:1328295/100‑1 (MQ=255) ctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCa > 2:563856/1‑100 (MQ=255) ctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCa > 1:1000376/1‑100 (MQ=255) cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc > 1:1019032/1‑100 (MQ=255) cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGAAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc > 1:1040892/1‑100 (MQ=255) gCGAACACTTTTCTGTGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAgg < 2:296584/100‑1 (MQ=255) gCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAgg < 2:138504/100‑1 (MQ=255) gAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCt > 2:1360199/1‑100 (MQ=255) aaCACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTa < 1:1169864/100‑1 (MQ=255) cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc > 1:724192/1‑100 (MQ=255) cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCt < 2:1820543/100‑1 (MQ=255) tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg > 2:263757/1‑100 (MQ=255) tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg > 1:96163/1‑100 (MQ=255) cGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaa < 1:988128/100‑1 (MQ=255) aTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc < 2:274866/100‑1 (MQ=255) aTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc < 2:724193/100‑1 (MQ=255) tGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc < 2:1862380/100‑1 (MQ=255) tGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc > 1:1021527/1‑100 (MQ=255) ggAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacca > 1:1095290/1‑100 (MQ=255) aGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGa < 2:1040893/100‑1 (MQ=255) tCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAg < 2:592034/100‑1 (MQ=255) gCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTg > 1:75052/1‑100 (MQ=255) ccGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGc < 2:1276396/100‑1 (MQ=255) cGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCt > 2:1452043/1‑100 (MQ=255) aCCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGa < 2:1000377/100‑1 (MQ=255) aCCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGa < 1:1402031/100‑1 (MQ=255) cATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACg < 2:131507/100‑1 (MQ=255) aTTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGt > 2:355425/1‑100 (MQ=255) aTTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGt > 2:823672/1‑100 (MQ=255) ttGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGt > 2:508501/1‑99 (MQ=255) tgacgaGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCg > 2:846030/1‑100 (MQ=255) | CTGGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACGTCG > NC_000913/459158‑459352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |