Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 661,369 T→C N4D (AAT→GAT)  ybeF ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913661,3690TC92.3% 276.8 / 3.4 91N4D (AAT→GAT) ybeFLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base C (49/35);  total (52/39)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTC  >  NC_000913/661272‑661465
                                                                                                 |                                                                                                
aTAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCAc                                                                                                <  2:1422010/100‑1 (MQ=255)
  aGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTa                                                                                              >  1:1182979/1‑100 (MQ=255)
   gTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTAt                                                                                             >  2:461481/1‑100 (MQ=255)
     cAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATcc                                                                                           >  1:819733/1‑100 (MQ=255)
     cAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATcc                                                                                           >  2:1866056/1‑100 (MQ=255)
        aaGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACg                                                                                        <  1:291063/100‑1 (MQ=255)
          ggTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCa                                                                                      >  2:922515/1‑100 (MQ=255)
          ggTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCa                                                                                      >  2:346789/1‑100 (MQ=255)
           gTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCAc                                                                                     >  2:1018655/1‑100 (MQ=255)
           gTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCAc                                                                                     >  1:1338941/1‑100 (MQ=255)
             tAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACtt                                                                                   >  1:1942114/1‑100 (MQ=255)
               aGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTAc                                                                                 >  2:998023/1‑100 (MQ=255)
               aGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTAc                                                                                 >  2:315278/1‑100 (MQ=255)
                gATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACt                                                                                >  2:1871472/1‑100 (MQ=255)
                gATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACt                                                                                >  1:1477164/1‑100 (MQ=255)
                gATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACt                                                                                >  2:895142/1‑100 (MQ=255)
                gATCAATATTTCGCAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACt                                                                                >  1:1203046/1‑100 (MQ=255)
                  tCAATATTTCGTAGGGTTGTAAATATTTGTGCTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTcc                                                                              >  2:1307472/1‑100 (MQ=255)
                    aaTATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCaa                                                                            >  2:12796/1‑100 (MQ=255)
                       atTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAAttt                                                                         <  1:709708/100‑1 (MQ=255)
                          tCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAt                                                                      <  2:1503400/100‑1 (MQ=255)
                          tCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAt                                                                      >  1:1058954/1‑100 (MQ=255)
                           cGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAtt                                                                     <  2:1073128/100‑1 (MQ=255)
                               aGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATg                                                                 <  1:1098711/100‑1 (MQ=255)
                               aGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATg                                                                 >  2:154887/1‑100 (MQ=255)
                                  ttGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGaa                                                              >  1:19402/1‑100 (MQ=255)
                                     tAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaaaaa                                                           <  2:227547/100‑1 (MQ=255)
                                     tAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaaaaa                                                           >  2:1936528/1‑100 (MQ=255)
                                         tatTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaataat                                                       <  2:340299/100‑1 (MQ=255)
                                            ttGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATAtt                                                    >  1:918239/1‑100 (MQ=255)
                                             tgtgGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAatat                                                     <  1:293039/98‑1 (MQ=255)
                                                  tttACCTTCTGATGACTTTCTGCTTAAACATGGTTCAATTTGATTATCACTATCCACTCACTTACTCCAATTTTATTCATGGAAAAATAATATTTaaaaa                                              <  1:1605470/100‑1 (MQ=255)
                                                  tttACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTaaaaa                                              >  2:1388759/1‑100 (MQ=255)
                                                    tACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAt                                            >  2:784359/1‑100 (MQ=255)
                                                    tACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAt                                            <  1:491453/100‑1 (MQ=255)
                                                     aCCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTAGTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAtt                                           >  2:145222/1‑100 (MQ=255)
                                                      ccTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTa                                          >  2:1650651/1‑100 (MQ=255)
                                                      ccTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTa                                          >  1:1095042/1‑100 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                         <  2:819734/100‑1 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                         >  1:1475132/1‑100 (MQ=255)
                                                        ttatGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATACAAGCACTTACTCCAATTTTATTCATGGAAAAATAAAAATAAAAAAATTAc                                         <  1:201549/96‑1 (MQ=255)
                                                        ttCTGATGACTTTCTCCTTAAACAGGTTTCAATTTGATTATTACTATACAAGTACTTACTCTAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                         <  1:324769/99‑1 (MQ=255)
                                                           tGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaata                                     >  1:48832/1‑100 (MQ=255)
                                                           tGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaata                                     >  2:355876/1‑100 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                    >  2:575132/1‑100 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                    >  1:1208696/1‑100 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                    >  2:484968/1‑100 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                    >  1:1815423/1‑100 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                  >  1:166898/1‑100 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                  >  2:1245587/1‑100 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                  >  2:269592/1‑100 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                  >  1:19620/1‑100 (MQ=255)
                                                               gacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCt                                 <  1:895141/100‑1 (MQ=255)
                                                               gacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCt                                 <  1:49342/100‑1 (MQ=255)
                                                                acTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCt                                 <  1:1263969/99‑1 (MQ=255)
                                                                acTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAAAAAAAATTAAAAAATTACAATAATCt                                 <  1:977221/99‑1 (MQ=255)
                                                                 cTTTCTGCTTAAACAGTGTTCAATTTGATTATCACTATCCACGCACTTACACCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                               <  1:1135314/100‑1 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                               <  1:1866044/100‑1 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                               <  1:1290900/100‑1 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                               <  1:985962/100‑1 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCATa                               >  1:1153497/1‑100 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACAATCCACGCACTTAATCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                               <  1:461479/100‑1 (MQ=255)
                                                                  tttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTAt                              <  2:986651/100‑1 (MQ=255)
                                                                  tttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTAt                              >  2:152247/1‑100 (MQ=255)
                                                                   ttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATg                             <  2:272986/100‑1 (MQ=255)
                                                                   ttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTACAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATg                             <  1:1550275/100‑1 (MQ=255)
                                                                   ttCTCCTTAAACAGGTTTCAATTTGATTATCACAATCCACGCACTTACTCCAATTATATTAATGGAAAAATAATATTTAAAAAATTACAATAATCTTAt                              <  1:692477/99‑1 (MQ=255)
                                                                    tattcTTAAACAGGGTTCAATTTGATTATTATTATAAAATCACTTACTCCAATTTTATTCTTGGAAAAATAATATTTAAAAAATTACAATAATCTTAt                              <  1:411474/94‑1 (MQ=255)
                                                                    tCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGt                            <  2:48931/100‑1 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCt                          <  2:387891/100‑1 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATCACTATACAAGCACTTACTCAAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCt                          <  1:12796/100‑1 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATCACAATCCAAGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTc                           <  1:1878199/99‑1 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTAATTATCACTATCCACGCACTTACTCCAATATAATAAATAGAAAAATAATATTTAAAAAATTACAATAATCTTATGt                            <  1:795060/98‑1 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTa                         >  2:1559532/1‑100 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTa                         >  2:1874669/1‑100 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTa                         >  2:890855/1‑100 (MQ=255)
                                                                         ttAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAAt                       >  1:1513388/1‑100 (MQ=255)
                                                                           aaaCAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTg                     >  2:1195419/1‑100 (MQ=255)
                                                                            aaCAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTgg                    <  2:1182981/100‑1 (MQ=255)
                                                                              cAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaa                  <  1:521788/100‑1 (MQ=255)
                                                                                gggTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacg                >  2:313484/1‑100 (MQ=255)
                                                                                 ggTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacg                >  1:202902/1‑99 (MQ=255)
                                                                                  gTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacgga              >  2:1338753/1‑100 (MQ=255)
                                                                                   ttCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacggaa             <  1:1074847/100‑1 (MQ=255)
                                                                                    tCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacggaac            <  1:1025380/100‑1 (MQ=255)
                                                                                    tCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacggaac            <  1:346787/100‑1 (MQ=255)
                                                                                       ntttGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCt         >  2:775893/2‑100 (MQ=255)
                                                                                       aTTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCt         >  2:1250026/1‑100 (MQ=255)
                                                                                       aTTTGATTATCACTATCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCtt        >  1:1447055/1‑100 (MQ=255)
                                                                                           gATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTg     >  1:1113450/1‑100 (MQ=255)
                                                                                           gATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTg     <  1:1766814/100‑1 (MQ=255)
                                                                                            attatcACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGc    <  2:1709710/100‑1 (MQ=255)
                                                                                            attatcACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGc    >  1:645547/1‑100 (MQ=255)
                                                                                              tatcaCTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTc  <  1:1218151/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGCTC  >  NC_000913/661272‑661465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: