| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 700,805 | A→T | C264S (TGC→AGC) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 700,805 | 0 | A | T | 98.7% | 262.7 / ‑3.9 | 74 | C264S (TGC→AGC) | nagC | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (26/47); total (27/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
TGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCC > NC_000913/700709‑700900 | tGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGcc > 2:376987/1‑100 (MQ=255)tGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGcc < 2:1168675/100‑1 (MQ=255)tGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGcc > 2:202078/1‑100 (MQ=255) ccgtagtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTCACAGACTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGc < 1:646492/92‑1 (MQ=255) cgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGtt < 2:288586/100‑1 (MQ=255) gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg > 2:160214/1‑100 (MQ=255) tcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGc < 1:928616/100‑1 (MQ=255) ccAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGcc < 2:581385/100‑1 (MQ=255) ccAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGc < 1:1749719/99‑1 (MQ=255) cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg < 2:1561480/100‑1 (MQ=255) ggCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGACAAAGTTGCCGCAGt > 2:1917562/1‑100 (MQ=255) gCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTg < 2:788257/100‑1 (MQ=255) gCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTg < 2:1105141/100‑1 (MQ=255) cACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTgg > 2:1010590/1‑100 (MQ=255) cACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTgg > 2:1907125/1‑100 (MQ=255) cACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTgg > 2:881613/1‑100 (MQ=255) cgcgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGc < 1:914655/99‑1 (MQ=255) gcgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAg < 1:555921/100‑1 (MQ=255) gCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCTCAGTGGCAGCGt < 2:1277243/100‑1 (MQ=255) ctctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGtt < 2:1628292/100‑1 (MQ=255) tGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAc < 2:566397/100‑1 (MQ=255) ggTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCAcc > 2:180307/1‑100 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCa < 2:205841/100‑1 (MQ=255) tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCa > 1:1477821/1‑100 (MQ=255) cccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCg < 1:1917316/100‑1 (MQ=255) cccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCg < 1:1264068/100‑1 (MQ=255) ccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCgg > 2:1859739/1‑100 (MQ=255) tGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGtt > 2:1218092/1‑100 (MQ=255) gCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTc > 1:609711/1‑100 (MQ=255) gCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTc < 2:1141392/100‑1 (MQ=255) gCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTc < 1:1772324/100‑1 (MQ=255) tttAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCg < 1:1896461/99‑1 (MQ=255) tAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc > 1:1321548/1‑100 (MQ=255) tAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc < 1:16450/100‑1 (MQ=255) aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt > 2:613941/1‑100 (MQ=255) aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg < 2:1679560/100‑1 (MQ=255) aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg > 1:1777927/1‑100 (MQ=255) naGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa > 1:325367/2‑100 (MQ=255) cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa < 2:369506/100‑1 (MQ=255) aTTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAata > 2:1197825/1‑100 (MQ=255) ttCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAatat > 1:1716069/1‑100 (MQ=255) cAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATgg < 2:327681/100‑1 (MQ=255) aaCACCCGTTATTCAATGTCAGCGTTGGCAGCGATCGTTTCCAGGCTGCCAAAGTTGCTGCAGCGGCAGCGTTCACCCAGCGGTTCGACCTGAATATgg > 1:930209/1‑99 (MQ=255) cacCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCa < 1:742834/100‑1 (MQ=255) cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt < 2:605136/100‑1 (MQ=255) gttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCGAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTc < 1:1248540/100‑1 (MQ=255) gttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTc > 1:1893779/1‑100 (MQ=255) ttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCa < 2:352118/100‑1 (MQ=255) gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc > 2:981718/1‑100 (MQ=255) gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc > 1:1696454/1‑100 (MQ=255) ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg > 1:1157380/1‑100 (MQ=255) tCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGa > 1:786562/1‑100 (MQ=255) aaTGGCAGCGTTGGCAGTGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAACTTCACCGAcg < 1:774352/100‑1 (MQ=255) aTGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgt < 1:210002/100‑1 (MQ=255) gCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgc > 2:1693091/1‑100 (MQ=255) gCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgc > 2:313159/1‑100 (MQ=255) cAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgcc < 2:1250761/100‑1 (MQ=255) aGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccn < 2:325369/100‑2 (MQ=255) aGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccg < 2:1477829/100‑1 (MQ=255) gTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgttg < 1:1917549/100‑1 (MQ=255) tGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCg < 2:1515571/100‑1 (MQ=255) tGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCg < 2:901567/100‑1 (MQ=255) tGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATCTCACCGACGTTGCCGTTGCg < 1:1186718/100‑1 (MQ=255) gCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGc < 2:1280768/100‑1 (MQ=255) aGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCa < 1:462441/100‑1 (MQ=255) gATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATa > 2:358471/1‑100 (MQ=255) gTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa < 1:1838951/100‑1 (MQ=255) gTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa < 2:800055/100‑1 (MQ=255) gTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACACAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa < 1:871270/100‑1 (MQ=255) ttCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATg < 1:1218089/100‑1 (MQ=255) tCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgc < 2:833057/100‑1 (MQ=255) cAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcgc < 1:215431/100‑1 (MQ=255) cAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcgc < 1:1859727/100‑1 (MQ=255) aGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc < 1:313157/100‑1 (MQ=255) | TGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCC > NC_000913/700709‑700900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |