| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 905,720 | C→A | L270M (CTG→ATG) | amiD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 905,720 | 0 | C | A | 96.4% | 281.3 / 1.0 | 85 | L270M (CTG→ATG) | amiD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (43/38); total (45/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCT > NC_000913/905624‑905816 | tccgatctcgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATgg < 2:311243/92‑1 (MQ=255)cagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACTTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATgg < 1:1772980/100‑1 (MQ=255)cagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATgg < 1:1244134/100‑1 (MQ=255) ggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGaa < 2:1656200/100‑1 (MQ=255) ggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGaa > 1:1754900/1‑100 (MQ=255) gcgTGATTATGGCATTCCAGATGCATTTCCACCAGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGGTGGAGAAATa > 1:1276314/1‑100 (MQ=255) cgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGgagatcgga > 1:311241/1‑95 (MQ=255) cgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATAc > 2:1471774/1‑100 (MQ=255) gTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACg > 2:1470854/1‑100 (MQ=255) gATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACggg < 1:1411386/100‑1 (MQ=255) tATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAg > 2:1272995/1‑100 (MQ=255) aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAgg > 2:1684416/1‑100 (MQ=255) ggCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAt > 2:805171/1‑100 (MQ=255) cATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTa > 2:1611666/1‑100 (MQ=255) ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc < 1:62505/100‑1 (MQ=255) aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGcgcg < 1:9144/99‑1 (MQ=255) gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGc < 1:1611656/100‑1 (MQ=255) aTGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCa > 2:590558/1‑100 (MQ=255) gCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGt > 1:1320949/1‑100 (MQ=255) cATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGCAACTCAGGCGATTCCCGAAGCATTGATGGAGAAATACGGGCCGGATTAGCGCGGCAGtt > 1:693485/1‑100 (MQ=255) tttCCGCCCGACGTTATTTAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGttt < 2:283809/99‑1 (MQ=255) ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTc < 2:305483/100‑1 (MQ=255) tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc > 2:863488/1‑100 (MQ=255) ccGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCg < 1:717831/100‑1 (MQ=255) ccGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCg < 1:1177311/100‑1 (MQ=255) cGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGt > 2:1004024/1‑100 (MQ=255) gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg > 1:1226814/1‑100 (MQ=255) ccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGt < 2:690056/100‑1 (MQ=255) cGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTc < 2:1809431/100‑1 (MQ=255) cGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTc < 2:1809730/100‑1 (MQ=255) cGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTc > 1:547265/1‑100 (MQ=255) ttATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTa < 1:1489328/100‑1 (MQ=255) tagataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAg < 1:532584/97‑1 (MQ=255) tataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGcc > 2:984309/1‑100 (MQ=255) tataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGcc > 2:431805/1‑100 (MQ=255) ataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGcc > 2:1189036/1‑99 (MQ=255) taACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAg < 1:321333/100‑1 (MQ=255) taACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAg > 1:1733911/1‑100 (MQ=255) aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg > 1:630701/1‑100 (MQ=255) aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg > 1:262999/1‑100 (MQ=255) aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg > 1:1237842/1‑100 (MQ=255) cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcg > 2:543424/1‑100 (MQ=255) cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcg < 2:560972/100‑1 (MQ=255) ggCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg > 2:622193/1‑100 (MQ=255) cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCa < 2:1237846/100‑1 (MQ=255) gAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAg < 1:1559113/100‑1 (MQ=255) aGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtt > 2:562/1‑100 (MQ=255) aGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtt < 2:833517/100‑1 (MQ=255) aGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATCAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtt > 1:1147547/1‑100 (MQ=255) gCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttt < 2:504029/100‑1 (MQ=255) cGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGttt < 2:564960/99‑1 (MQ=255) ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc < 1:1898288/100‑1 (MQ=255) ccgatctgAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc < 1:1789277/93‑1 (MQ=255) gATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtct > 1:1070488/1‑100 (MQ=255) tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg < 2:263001/100‑1 (MQ=255) tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg < 1:1470846/100‑1 (MQ=255) gCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa > 1:1625357/1‑100 (MQ=255) cAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt < 2:533635/100‑1 (MQ=255) cAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGATTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt > 1:1094989/1‑100 (MQ=255) gAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtcagatcgg > 2:1789288/1‑93 (MQ=255) aaCTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt > 1:1593616/1‑100 (MQ=255) aCTCAGGCGATTGCCGAAGCATTGATGGTGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt < 1:974876/100‑1 (MQ=255) aCTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt < 1:1745734/100‑1 (MQ=255) cTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc < 2:1593627/100‑1 (MQ=255) cTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc > 1:126686/1‑100 (MQ=255) tCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCa < 1:704352/100‑1 (MQ=255) aGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATc < 2:1783141/100‑1 (MQ=255) ggCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc > 2:1863510/1‑100 (MQ=255) gCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa < 1:1544691/100‑1 (MQ=255) gATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAgg > 2:732320/1‑100 (MQ=255) aTTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg < 1:1471766/100‑1 (MQ=255) tGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtg > 2:792551/1‑100 (MQ=255) gCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga > 2:950558/1‑100 (MQ=255) ccGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgat > 1:1733516/1‑100 (MQ=255) gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatgn < 1:451600/100‑2 (MQ=255) gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga > 1:1061963/1‑100 (MQ=255) gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga > 1:192038/1‑100 (MQ=255) gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga > 1:84419/1‑100 (MQ=255) gAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga > 2:1866061/1‑100 (MQ=255) aGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAcc < 2:1149957/100‑1 (MQ=255) aGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAcc > 2:1272274/1‑100 (MQ=255) gCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCg < 2:979915/100‑1 (MQ=255) cATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg > 1:501573/1‑100 (MQ=255) ttGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt < 2:556410/100‑1 (MQ=255) ttGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt > 2:1522114/1‑100 (MQ=255) | CAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCT > NC_000913/905624‑905816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |