| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,097,209 | T→G | intergenic (+380/+356) | ycdU → / ← serX | putative inner membrane protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,209 | 0 | T | G | 87.5% | 56.4 / 1.5 | 33 | intergenic (+380/+356) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base G (13/15); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTC > NC_000913/1097113‑1097255 | gTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGa > 2:1323127/1‑99 (MQ=25)cccgaccgggggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAg > 2:58414/11‑100 (MQ=11) cGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAggg > 2:1863917/1‑100 (MQ=255) gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAgggg < 1:240623/100‑1 (MQ=25) gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAgggg > 2:1931068/1‑100 (MQ=255) cccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAgggggg < 2:29232/100‑1 (MQ=18) ccccGGTCGGGGCTTCGCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAggggggg < 2:182165/100‑1 (MQ=12) ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAggggggg > 2:1720706/1‑98 (MQ=18) cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGAtt < 2:1094174/100‑1 (MQ=18) ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTg < 2:1196088/100‑1 (MQ=17) tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGAc < 1:1593297/100‑1 (MQ=32) agggCTGCTCAGGCACCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtc < 1:200722/99‑1 (MQ=11) gggCTTCTCATCCCCCCGGTGTGTGCAATACCCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcg < 1:980899/100‑1 (MQ=12) gggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcg < 1:1931055/100‑1 (MQ=18) ggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgc > 1:835956/1‑100 (MQ=18) gCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgct < 1:1610435/100‑1 (MQ=17) ttCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttc < 1:58414/100‑1 (MQ=18) tgtcATCCCCCCGATGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttcg > 2:1318829/3‑100 (MQ=255) tctcATCCCCCCTGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttcg < 1:37073/100‑1 (MQ=12) ctcATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttcgc > 1:947226/1‑100 (MQ=18) cATCCGCCGGGAGTGGGCAATACCCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTtcgctc < 2:1605204/100‑1 (MQ=11) cATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTtcgctc < 2:434822/100‑1 (MQ=18) cATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTtcgctc > 2:1558582/1‑100 (MQ=18) aTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTtcgctcg < 1:146920/100‑1 (MQ=18) tCCCCCCTGTTTGTAAAATACACGAGGCAAACGCCCGTACCTGCGTACGAGCGCTTCTTTGAGTGTGGCGGCGAGGTGGGGATTGACTCGCTtcgctcgc < 2:8742/100‑1 (MQ=2) tCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTtcgctc > 1:1943962/1‑98 (MQ=18) ccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGcc < 1:958676/100‑1 (MQ=17) ccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCt > 2:1056870/1‑100 (MQ=18) tgtgtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTAGCCCTGCGGGc > 1:747426/1‑100 (MQ=18) gCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc > 2:1415658/1‑100 (MQ=18) gCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc > 1:737632/1‑100 (MQ=18) aTACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGc < 1:1056869/99‑1 (MQ=32) tACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTc < 2:476972/100‑1 (MQ=17) | GTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTC > NC_000913/1097113‑1097255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |