| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 129,360 | G→T | intergenic (+24/+47) | lpd → / ← yacH | dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 129,360 | 0 | G | T | 93.0% | 232.5 / 9.3 | 71 | intergenic (+24/+47) | lpd/yacH | dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base T (34/32); total (36/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACT > NC_000913/129264‑129455 | tgtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCaa > 1:227838/1‑100 (MQ=255)tgtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCaa > 2:475893/1‑100 (MQ=255) gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAt > 2:852017/1‑100 (MQ=255) gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAt > 2:80317/1‑100 (MQ=255) gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAAt > 2:1128451/1‑100 (MQ=255) tgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAtt < 2:580265/100‑1 (MQ=255) ccTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaa > 2:1547728/1‑100 (MQ=255) cTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaaa < 2:797448/100‑1 (MQ=255) ggcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAg > 1:918408/1‑100 (MQ=255) ggcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAg > 2:1152180/1‑100 (MQ=255) cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCg > 2:53472/1‑100 (MQ=255) cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCg < 2:304743/100‑1 (MQ=255) ggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCgg > 2:1346366/1‑100 (MQ=255) gcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGc < 2:1240521/100‑1 (MQ=255) gcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGc < 2:559446/100‑1 (MQ=255) cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCt > 1:1940727/1‑100 (MQ=255) cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCt > 2:433494/1‑100 (MQ=255) tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc < 2:498454/100‑1 (MQ=255) tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc < 2:1939445/100‑1 (MQ=255) tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc < 2:1451979/100‑1 (MQ=255) aGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtt > 1:1630610/1‑100 (MQ=255) aGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtt > 1:153173/1‑100 (MQ=255) ggTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttt < 1:1927467/100‑1 (MQ=255) gTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtttt > 2:1244508/1‑100 (MQ=255) tAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCGATTAAAAAAGCGGCTAACCACGCCGCttttt > 2:745980/1‑100 (MQ=255) gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt > 1:33777/1‑100 (MQ=255) gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt > 1:1070684/1‑100 (MQ=255) aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc < 2:284876/100‑1 (MQ=255) tACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGt > 1:54944/1‑100 (MQ=255) aCCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc > 1:1256435/1‑100 (MQ=255) aCCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc > 1:997164/1‑100 (MQ=255) aCCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCa > 2:1201799/1‑100 (MQ=255) tGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAtt < 1:142097/100‑1 (MQ=255) gCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt < 1:1860314/100‑1 (MQ=255) ccGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGTAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTa < 1:694500/100‑1 (MQ=255) cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc < 2:1766413/100‑1 (MQ=255) gAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAcc > 1:1520876/1‑100 (MQ=255) aCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCtt < 2:1190829/100‑1 (MQ=255) aCCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCtt < 2:153174/100‑1 (MQ=255) cccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTCACCACGCCGCTTTTTTTACGTCTGCAATTTACCttt < 1:305146/100‑1 (MQ=255) cccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCttt > 2:537699/1‑100 (MQ=255) ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc < 1:1244504/100‑1 (MQ=255) cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc > 2:939891/1‑100 (MQ=255) gCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtct < 1:175417/100‑1 (MQ=255) gCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtct > 2:1494643/1‑100 (MQ=255) cGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtctt < 1:80317/100‑1 (MQ=255) cGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtctt > 1:254299/1‑100 (MQ=255) gaagaagaaGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc > 2:1458179/1‑100 (MQ=255) gaagaagaaGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttc < 1:449516/100‑1 (MQ=255) agaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTcttctt > 2:492636/1‑100 (MQ=255) agaagaagTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTcttctt < 1:43457/100‑1 (MQ=255) aagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGc < 1:1121389/100‑1 (MQ=255) agaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCt > 1:576982/1‑100 (MQ=255) gTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCAc < 1:335818/100‑1 (MQ=255) aTTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCCCCACGtt > 2:405988/1‑100 (MQ=255) tttttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTc > 2:533484/1‑100 (MQ=255) ttttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCa < 1:1346358/100‑1 (MQ=255) tttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCag < 1:1439628/100‑1 (MQ=255) tttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCag < 1:1152178/100‑1 (MQ=255) tCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagag < 2:940828/100‑1 (MQ=255) cGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagaga < 2:1257292/100‑1 (MQ=255) ttGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGAc < 2:227840/100‑1 (MQ=255) tGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACg > 2:754847/1‑100 (MQ=255) gCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt > 1:276631/1‑100 (MQ=255) ccGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACg < 1:73276/98‑1 (MQ=255) ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg < 2:54944/100‑1 (MQ=255) ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg < 2:634270/100‑1 (MQ=255) ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg < 2:918409/100‑1 (MQ=255) aaCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCa < 1:101208/100‑1 (MQ=255) aTCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATAc > 2:1020914/1‑100 (MQ=255) tCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATAct > 2:1498229/1‑100 (MQ=255) | TGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACT > NC_000913/129264‑129455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |