Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,100,233 G→A intergenic (+39/‑63) ycdY → / → ycdZ redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,100,2330GA98.6% 234.9 / ‑4.3 70intergenic (+39/‑63)ycdY/ycdZredox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (37/32);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

TAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGG  >  NC_000913/1100136‑1100330
                                                                                                 |                                                                                                 
tAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttatt                                                                                                 >  2:147430/1‑100 (MQ=255)
tAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttatt                                                                                                 >  1:909958/1‑100 (MQ=255)
 aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                                >  2:1563330/1‑100 (MQ=255)
 aaCCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattg                                                                                                >  1:15566/1‑100 (MQ=255)
   cccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgtt                                                                                              >  1:495092/1‑100 (MQ=255)
    tagcggtGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                             <  1:1006418/94‑1 (MQ=255)
    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                             <  2:1886966/100‑1 (MQ=255)
    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                             <  1:1332613/100‑1 (MQ=255)
    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                             <  1:602967/100‑1 (MQ=255)
    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                             <  1:243207/100‑1 (MQ=255)
    ccGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACAGATAATttgttgttg                                                                                             >  2:1911503/1‑100 (MQ=255)
     cgcgATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttattgttg                                                                                             <  1:1448561/99‑1 (MQ=255)
        gATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATa                                                                                         <  2:505378/100‑1 (MQ=255)
        gATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATa                                                                                         >  1:357993/1‑100 (MQ=255)
             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                    >  1:1594230/1‑100 (MQ=255)
             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                    >  2:458720/1‑100 (MQ=255)
             cATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtg                                                                                    >  2:242795/1‑100 (MQ=255)
              aTTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAt                                                                                     <  1:448984/98‑1 (MQ=255)
                tAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgt                                                                                 <  2:926408/100‑1 (MQ=255)
                tAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAAtgtgt                                                                                 >  1:382015/1‑100 (MQ=255)
                   tGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGc                                                                               <  1:1866420/99‑1 (MQ=255)
                    gCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTc                                                                             >  1:1410650/1‑100 (MQ=255)
                     cAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCtag                                                                            >  1:1179933/1‑98 (MQ=255)
                      aaTGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCg                                                                            <  1:602787/99‑1 (MQ=255)
                      aaTGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCg                                                                            >  2:131300/1‑99 (MQ=255)
                      aaTGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGa                                                                           <  1:583857/100‑1 (MQ=255)
                       aTGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAt                                                                          >  2:1038502/1‑100 (MQ=255)
                        tgtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtc                                                                         <  2:1865526/100‑1 (MQ=255)
                         gtgGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGAtct                                                                        <  2:1705416/100‑1 (MQ=255)
                            ggACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcat                                                                     >  2:230741/1‑100 (MQ=255)
                              aCGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTTCTCGATCtcattc                                                                   <  2:1318718/100‑1 (MQ=255)
                                gAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattcat                                                                 <  2:1087929/100‑1 (MQ=255)
                                gAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCtcattcat                                                                 <  2:15566/100‑1 (MQ=255)
                                    tGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGCTCTCATTCCTggtc                                                             >  2:1902002/1‑98 (MQ=255)
                                    tGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGcc                                                             >  1:628202/1‑100 (MQ=255)
                                     ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                                            >  1:1468712/1‑100 (MQ=255)
                                     ggAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                                            >  1:1937846/1‑100 (MQ=255)
                                       aagaagATTCTGAAGAATAAGTGTGATCTACGTCACTTATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcgcg                                                          <  1:356560/100‑1 (MQ=255)
                                       aagaagATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcgcg                                                          <  1:791294/100‑1 (MQ=255)
                                         gaagaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtt                                                        >  2:1425756/1‑100 (MQ=255)
                                           agaTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGtttt                                                      >  2:1919979/1‑100 (MQ=255)
                                             aTTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCt                                                    >  1:96423/1‑100 (MQ=255)
                                              ttCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTg                                                   <  1:41070/100‑1 (MQ=255)
                                               tCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGc                                                  <  2:408914/100‑1 (MQ=255)
                                                cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCt                                                 >  1:1518754/1‑100 (MQ=255)
                                                cTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATCGCCGCGTTTTCTGCt                                                 >  2:1912318/1‑100 (MQ=255)
                                                 tGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTa                                                >  1:374046/1‑100 (MQ=255)
                                                  gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt                                               <  2:1199029/100‑1 (MQ=255)
                                                  gaagaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTAt                                               <  1:1901989/100‑1 (MQ=255)
                                                     gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCAt                                            >  1:1593683/1‑100 (MQ=255)
                                                     gaaTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCAt                                            >  1:551058/1‑100 (MQ=255)
                                                       aTAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgc                                          >  2:1082740/1‑100 (MQ=255)
                                                           gtgtGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCgg                                      >  2:253723/1‑100 (MQ=255)
                                                           gtgtGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCgg                                      >  1:1813011/1‑100 (MQ=255)
                                                           gtgtGATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCgg                                      >  2:1508041/1‑100 (MQ=255)
                                                             gtgATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCa                                    <  1:1501117/100‑1 (MQ=255)
                                                              tgATCTACGTCACTTATAACTGCAACGGATAATTTATTGTTGCATAAGATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCAt                                   <  2:362398/100‑1 (MQ=255)
                                                               gATCTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                                  >  2:241728/1‑100 (MQ=255)
                                                                  cTACGTCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAAc                               <  2:1387598/100‑1 (MQ=255)
                                                                       tCACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACt                          <  2:184077/100‑1 (MQ=255)
                                                                        cACTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGCTCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACtt                         >  1:422847/1‑100 (MQ=255)
                                                                          cTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtct                       >  1:650166/1‑100 (MQ=255)
                                                                          cTCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtct                       >  1:722400/1‑100 (MQ=255)
                                                                           tCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctc                      <  2:650167/100‑1 (MQ=255)
                                                                           tCATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctc                      <  1:147429/100‑1 (MQ=255)
                                                                            cATAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctc                      <  1:925409/99‑1 (MQ=255)
                                                                             aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtctctc                    <  2:147941/100‑1 (MQ=255)
                                                                             aTAACTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGCTTTCTGCTATCATGCGCGGCATGAACATACTtctctc                    <  1:1038501/100‑1 (MQ=255)
                                                                                aCTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAt                 >  2:1814081/1‑100 (MQ=255)
                                                                                aCTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAt                 >  2:1358606/1‑100 (MQ=255)
                                                                                 cTGCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAtt                <  2:1626365/100‑1 (MQ=255)
                                                                                 cTGCAACGGATAATTTATTGTTGCATAAAACGTATGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACCTCTCTCTAtt                >  1:1281923/1‑100 (MQ=255)
                                                                                   gCAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGc              >  1:174185/1‑100 (MQ=255)
                                                                                    cAACGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCa             >  2:394403/1‑100 (MQ=255)
                                                                                      aCGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAAt           >  2:1416540/1‑100 (MQ=255)
                                                                                       cGGATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATc          <  1:35257/100‑1 (MQ=255)
                                                                                         gATAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATcac        <  1:797694/100‑1 (MQ=255)
                                                                                          aTAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATcaca       <  2:775171/100‑1 (MQ=255)
                                                                                          aTAATTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATcaca       <  1:1082739/100‑1 (MQ=255)
                                                                                            aaTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaac     >  2:1252174/1‑100 (MQ=255)
                                                                                            aaTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCacaac     <  1:458718/100‑1 (MQ=255)
                                                                                             aTTTATTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCccaaca    >  1:1420910/1‑100 (MQ=255)
                                                                                               ttgttgttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAgg  <  2:971067/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
TAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGG  >  NC_000913/1100136‑1100330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: