| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,197,340 | T→C | T65A (ACT→GCT) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,197,340 | 0 | T | C | 95.1% | 265.9 / 2.5 | 82 | T65A (ACT→GCT) | ymfD | e14 prophage; putative SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base C (38/40); total (39/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
CATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATA > NC_000913/1197244‑1197436 | cATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAAc > 1:1639667/1‑100 (MQ=255)cATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCC‑TTTCGAGTCTAGAAAAGCAACt > 1:1496983/1‑100 (MQ=255) aTTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACt < 2:1883143/100‑1 (MQ=255) tttATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTtca < 2:1196720/100‑1 (MQ=255) ttATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTtcat > 1:260720/1‑100 (MQ=255) ataataTCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTtcatca < 2:937208/100‑1 (MQ=255) atatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAAt > 2:301227/1‑100 (MQ=255) tatCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAAtt > 2:682589/1‑100 (MQ=255) atCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAAttt < 1:301225/100‑1 (MQ=255) gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACt > 2:922396/1‑100 (MQ=255) gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACt > 1:366449/1‑100 (MQ=255) gTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACt > 1:1249474/1‑100 (MQ=255) gACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGAtt > 1:1508924/1‑100 (MQ=255) gACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGAtt < 2:368167/100‑1 (MQ=255) aCATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGAtt < 1:1933580/99‑1 (MQ=255) aCATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTa < 2:1853265/100‑1 (MQ=255) cATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTan < 2:276389/100‑2 (MQ=255) aTAGTCAATAATTTTAATATTCATCCCTCTAATAATCTACTCTCTTTCAACTAGCCTTCTCGATTCTAGACAAGCAACTTCCTCAAACTTACTGCCTAAt < 1:1154030/100‑1 (MQ=255) tAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAAtt < 1:1269428/100‑1 (MQ=255) tAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAAtt < 1:321049/100‑1 (MQ=255) aGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAAtt > 2:1750768/1‑99 (MQ=255) aGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtc > 1:1085834/1‑100 (MQ=255) gTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATtca < 1:219698/100‑1 (MQ=255) gTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtca > 1:228791/1‑100 (MQ=255) tCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtcat < 1:1668264/100‑1 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtcatc < 1:682588/100‑1 (MQ=255) cAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtcatc > 2:456309/1‑100 (MQ=255) aataatTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATtcatca > 2:718686/1‑100 (MQ=255) ttttAGTTTTAATTTCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAata < 1:1286911/100‑1 (MQ=255) ttttAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAata > 2:685595/1‑100 (MQ=255) ttAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATc < 2:41092/100‑1 (MQ=255) aGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTa < 2:228793/100‑1 (MQ=255) aGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTa < 2:1810683/100‑1 (MQ=255) ttttAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAg < 2:1797564/100‑1 (MQ=255) ttttAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATAAAATTTACTGATGAATTCATCAGAATATCTAAg < 1:442159/100‑1 (MQ=255) ttAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGtt > 2:1056967/1‑100 (MQ=255) ttCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTcc < 1:1517170/100‑1 (MQ=255) ttCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTcc < 1:1015074/100‑1 (MQ=255) ttCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTcc < 1:744807/100‑1 (MQ=255) tCCTCTAATAATTTGCTCTCTTTCAATTTGCCTTTTCGAGTCTATAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCg < 2:263773/100‑1 (MQ=255) ccTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGc > 2:895866/1‑100 (MQ=255) ccTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGc > 1:555267/1‑100 (MQ=255) ctctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCa < 2:799972/100‑1 (MQ=255) tctAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAg > 2:366667/1‑100 (MQ=255) atTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAAt < 2:1508932/100‑1 (MQ=255) tttGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATc < 2:1749976/100‑1 (MQ=255) tGCTCTCTTTCAAGTTTCCTTTTCGAGTCTAGAAAATCAACTTCATCAAATTTACTGATTAAGTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCaa > 2:575737/1‑100 (MQ=255) gCTCTCTTTCAAGTTGCCCTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAg < 1:1664340/100‑1 (MQ=255) ctctctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAgg > 1:1300260/1‑100 (MQ=255) tctctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAggg < 1:891262/100‑1 (MQ=255) ctTTCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGTAGCCAAAATCAAGGGCGt < 1:456307/100‑1 (MQ=255) tCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGc > 2:554220/1‑100 (MQ=255) tCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGc > 1:1924880/1‑100 (MQ=255) tCAAGTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGc > 2:1730027/1‑100 (MQ=255) gTTGCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAtt > 2:73835/1‑100 (MQ=255) gCCTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCAttttt > 1:1431066/1‑100 (MQ=255) ccTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc < 2:471687/100‑1 (MQ=255) ccTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc < 1:979407/100‑1 (MQ=255) ccTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtc < 2:803882/100‑1 (MQ=255) cTTTTCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtct > 1:888659/1‑100 (MQ=255) ttttCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtctc < 2:1249478/100‑1 (MQ=255) ttttCGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTtctc < 1:718685/100‑1 (MQ=255) ttCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCaa < 2:1256891/100‑1 (MQ=255) cGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATg > 1:395209/1‑100 (MQ=255) cGAGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATg > 1:1602934/1‑100 (MQ=255) aGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCt < 2:1639677/100‑1 (MQ=255) aGTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCt < 1:116257/100‑1 (MQ=255) gTCTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCtt > 1:1811096/1‑100 (MQ=255) cTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCt > 1:1438293/1‑100 (MQ=255) cTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCt < 2:1688621/100‑1 (MQ=255) cTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCt > 1:1648246/1‑100 (MQ=255) cTAGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCt < 2:1710780/100‑1 (MQ=255) aGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTaa < 1:922395/100‑1 (MQ=255) aGAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTaa < 1:728192/100‑1 (MQ=255) gAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat < 2:674233/100‑1 (MQ=255) gAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat > 1:994635/1‑100 (MQ=255) gAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat > 1:1451641/1‑100 (MQ=255) gAAAAGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAat > 2:913492/1‑100 (MQ=255) aaaGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAatat > 2:1052634/1‑100 (MQ=255) aaaGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAatat > 1:1719420/1‑100 (MQ=255) aaaGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAatat > 1:1148214/1‑100 (MQ=255) aaGCAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAAtata > 1:1042526/1‑100 (MQ=255) | CATTTTTATAATATCGTGGGACATAGTCAATAATTTTAGTTTTAATTCCTCTAATAATTTGCTCTCTTTCAAGTTGCCTTTTCGAGTCTAGAAAAGTAACTTCATCAAATTTACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATA > NC_000913/1197244‑1197436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |