Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,197,739 A→G S167P (TCC→CCC)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,7390AG88.2% 281.5 / 21.1 103S167P (TCC→CCC) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (7/5);  new base G (47/43);  total (54/49)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

Only 100 of 113 total aligned reads displayed.

CACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTT  >  NC_000913/1197641‑1197833
                                                                                                  |                                                                                              
cactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAggaaggaa                                                                                               >  2:1682881/1‑100 (MQ=255)
    gagaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTa                                                                                           >  1:254214/1‑100 (MQ=255)
     agaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTaa                                                                                          >  2:1157076/1‑100 (MQ=255)
      gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAAt                                                                                         >  2:228058/1‑100 (MQ=255)
       aaTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATg                                                                                        <  2:1326541/100‑1 (MQ=255)
         tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCCTTTGTTTGCAAAAAAAGATTATTAGGCAGGGAATTAATGCa                                                                                      >  2:1705954/1‑100 (MQ=255)
         tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                      >  2:1809361/1‑100 (MQ=255)
         tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                      >  2:1157979/1‑100 (MQ=255)
         tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                      >  2:1541355/1‑100 (MQ=255)
           aTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAt                                                                                    >  2:1458197/1‑100 (MQ=255)
            tAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAtt                                                                                   <  2:1241802/100‑1 (MQ=255)
             aaaaTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTa                                                                                  <  2:1051343/100‑1 (MQ=255)
             aaaaTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTa                                                                                  >  1:378659/1‑100 (MQ=255)
                 ttACAAAAAAGAATAAAGACAGCAAAGAACCTTGAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATCATTAGGAAGGAAATTAATGCAATTATCGa                                                                              >  1:1173230/1‑100 (MQ=255)
                    cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                           >  2:34054/1‑100 (MQ=255)
                    cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                           >  1:1342942/1‑100 (MQ=255)
                    cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                           >  2:1108546/1‑100 (MQ=255)
                     aaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAt                                                                          >  2:93024/1‑100 (MQ=255)
                      aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt                                                                         <  1:561703/100‑1 (MQ=255)
                       aaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc                                                                        <  2:937576/100‑1 (MQ=255)
                       aaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc                                                                        <  1:254343/100‑1 (MQ=255)
                       aaaaGAATAAAGACAGCAAAGAACCTTAAACATGAAATATATCTATTTGTTTGCAAAAAAAGATTATTAGGAAGGTAATTAATGCAATTATCGAAAATTc                                                                        <  1:1341638/100‑1 (MQ=255)
                          aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                     <  2:617696/100‑1 (MQ=255)
                          aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                     <  2:441846/100‑1 (MQ=255)
                          aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                     >  2:1124467/1‑100 (MQ=255)
                           gAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaa                                                                    <  2:211511/100‑1 (MQ=255)
                            aaTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaa                                                                   >  2:416832/1‑100 (MQ=255)
                            aaTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaa                                                                    >  1:377368/1‑99 (MQ=255)
                             aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaaa                                                                  <  2:396431/100‑1 (MQ=255)
                               aaaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata                                                                <  2:1856608/100‑1 (MQ=255)
                               aaaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAata                                                                <  2:1142106/100‑1 (MQ=255)
                                  gACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATcc                                                             <  1:572809/100‑1 (MQ=255)
                                    cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                           >  1:1429185/1‑100 (MQ=255)
                                    cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                           >  1:1233747/1‑100 (MQ=255)
                                     aGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaa                                                          >  1:595052/1‑100 (MQ=255)
                                      gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                         >  1:1253298/1‑100 (MQ=255)
                                      gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                         >  2:1480195/1‑100 (MQ=255)
                                        aaaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAAt                                                       >  1:987525/1‑100 (MQ=255)
                                         aaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                      >  2:414460/1‑100 (MQ=255)
                                         aaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                      >  2:1785714/1‑100 (MQ=255)
                                           gAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGt                                                    >  1:100557/1‑100 (MQ=255)
                                             nccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtat                                                  >  1:937311/2‑100 (MQ=255)
                                             aCCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtat                                                  >  2:1143357/1‑100 (MQ=255)
                                               cTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATAc                                                <  2:594333/100‑1 (MQ=255)
                                                ttCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACt                                               >  1:1848190/1‑100 (MQ=255)
                                                ttCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACt                                               >  2:373802/1‑100 (MQ=255)
                                                   aaCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACttt                                             <  1:1599447/99‑1 (MQ=255)
                                                   aaCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACtt                                              <  1:93024/98‑1 (MQ=255)
                                                    aCATTAAAAATATCCAGTTGTTTGCAAAAAAACATTATTAGGAAGTGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                           <  2:669846/100‑1 (MQ=255)
                                                    aCATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                           >  1:1194676/1‑100 (MQ=255)
                                                     cATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAtt                                          <  2:254216/100‑1 (MQ=255)
                                                     cATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAtt                                          >  1:36910/1‑100 (MQ=255)
                                                     cATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAtt                                          >  1:322080/1‑100 (MQ=255)
                                                      aTGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAtt                                          <  2:1283487/99‑1 (MQ=255)
                                                        gAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                       <  2:1265986/100‑1 (MQ=255)
                                                         aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag                                      <  2:1572986/100‑1 (MQ=255)
                                                         aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag                                      <  1:714892/100‑1 (MQ=255)
                                                         aaaaaTATCCAATTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTAAAAAAAAAAAAAAAAATATCCAAAAATAGTATACTTTATTCCag                                      <  1:1809350/100‑1 (MQ=255)
                                                          aaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                     <  1:476385/100‑1 (MQ=255)
                                                          aaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                     <  2:879733/100‑1 (MQ=255)
                                                          aaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                     >  1:1012929/1‑100 (MQ=255)
                                                             atatCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaaga                                  >  2:1512022/1‑100 (MQ=255)
                                                                 ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                              <  1:164050/100‑1 (MQ=255)
                                                                  cATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCa                             >  1:1048686/1‑100 (MQ=255)
                                                                   aTTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa                            <  2:816853/100‑1 (MQ=255)
                                                                   aTTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTAAAAAAAAAAAAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa                            <  1:907526/100‑1 (MQ=255)
                                                                   aTTTGTTTGAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATAAAAAAAAAAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCaa                            <  1:366456/100‑1 (MQ=255)
                                                                    tttgtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                           <  2:1343427/100‑1 (MQ=255)
                                                                    tttgtttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                           <  1:1250364/100‑1 (MQ=255)
                                                                    tttgtttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                           <  1:999979/100‑1 (MQ=255)
                                                                     ttgtttgCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAata                          <  1:1577792/100‑1 (MQ=255)
                                                                       gtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAAtata                        >  1:1859014/1‑100 (MQ=255)
                                                                       gtttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAAtata                        >  2:1507585/1‑100 (MQ=255)
                                                                       gtttaaaaaaaaaGATTATTAGGAAGGGAATTAATGCAATTAAAAAAAAAAAAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAata                          <  1:1670549/92‑1 (MQ=255)
                                                                        tttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATaa                       <  1:418364/100‑1 (MQ=255)
                                                                         ttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAAt                      >  1:249217/1‑100 (MQ=255)
                                                                         ttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAAt                      <  1:1214604/100‑1 (MQ=255)
                                                                           gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGGAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                    >  1:1066409/1‑100 (MQ=255)
                                                                           gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                    <  2:691894/100‑1 (MQ=255)
                                                                            cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCTAAAATTAAAAAAAAAAAAAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                           <  1:190276/92‑1 (MQ=255)
                                                                            cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                   >  2:1758826/1‑100 (MQ=255)
                                                                            cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                   >  1:293355/1‑100 (MQ=255)
                                                                            cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                   >  2:854167/1‑100 (MQ=255)
                                                                             aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGttt                  >  2:1239598/1‑100 (MQ=255)
                                                                             aaaaaaaGATAATTAGGAAGGGCATTAATGCAATTATCGAAAATTCAAAAAATTTACAAAAATAGTATCATTTATTCCAGAAAAATTAAAAAAAAAGttt                  >  1:419232/1‑100 (MQ=255)
                                                                              aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                 <  1:927330/100‑1 (MQ=255)
                                                                              aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                 <  1:162991/100‑1 (MQ=255)
                                                                               aaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGt                >  2:933546/1‑100 (MQ=255)
                                                                                aaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTc               >  1:392033/1‑100 (MQ=255)
                                                                                  aaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCtt             <  1:1115977/100‑1 (MQ=255)
                                                                                  aaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCtt             <  1:1114858/100‑1 (MQ=255)
                                                                                       tattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtt        >  2:1619733/1‑100 (MQ=255)
                                                                                        attaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAttt       >  1:1105742/1‑100 (MQ=255)
                                                                                           aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc    <  1:1108545/100‑1 (MQ=255)
                                                                                           aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc    >  1:388363/1‑100 (MQ=255)
                                                                                           aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc    <  1:1358796/100‑1 (MQ=255)
                                                                                           aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCAAGAAGAGTTCAATATAATGTTTGTCTTCAAttttt     >  1:1740823/1‑99 (MQ=255)
                                                                                            ggaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCt   >  1:658670/1‑100 (MQ=255)
                                                                                             gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt  >  2:130958/1‑100 (MQ=255)
                                                                                             gaaggaaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCATAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt  <  2:1368679/100‑1 (MQ=255)
                                                                                                  |                                                                                              
CACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTT  >  NC_000913/1197641‑1197833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: