Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,677 C→T intergenic (‑151/+44) fnr ← / ← ogt oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,6770CT92.2% 300.4 / 12.7 103intergenic (‑151/+44)fnr/ogtoxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base T (47/47);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 104 total aligned reads displayed.

GAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTC  >  NC_000913/1399578‑1399773
                                                                                                   |                                                                                                
gAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAAc                                                                                                  <  1:46265/100‑1 (MQ=255)
     agtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTAc                                                                                             <  1:1782345/100‑1 (MQ=255)
     agtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTAc                                                                                             <  1:424504/100‑1 (MQ=255)
      gtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACa                                                                                            <  2:19311/100‑1 (MQ=255)
      gtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACa                                                                                            <  2:924242/100‑1 (MQ=255)
            cTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAg                                                                                      >  1:1737071/1‑100 (MQ=255)
            cTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAg                                                                                      >  2:1611502/1‑100 (MQ=255)
             tAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAgt                                                                                     >  2:300996/1‑100 (MQ=255)
              aaGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAg                                                                                      >  1:1001947/1‑98 (MQ=255)
                gCAAACCTTAACAGCAGAGCATTCCGATATTCGATGTAAATATATGTCTATCTATTTGAAAACCCTTCCGCTGTTAAGGGTAATTTTACATAAAAGtgtg                                                                                  <  1:1848440/100‑1 (MQ=255)
                gCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGtgtg                                                                                  <  2:31104/100‑1 (MQ=255)
                gCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGtgtg                                                                                  <  2:972956/100‑1 (MQ=255)
                gCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGtgtg                                                                                  >  1:1114301/1‑100 (MQ=255)
                 cAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGa                                                                                 <  1:415295/100‑1 (MQ=255)
                 cAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGa                                                                                 <  2:744525/100‑1 (MQ=255)
                 cAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGa                                                                                 <  2:748532/100‑1 (MQ=255)
                  aaaCCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGaa                                                                                >  1:1848637/1‑100 (MQ=255)
                     ccTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACaa                                                                             >  1:497790/1‑100 (MQ=255)
                       ttAACAGCAGATAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGc                                                                           <  1:456983/100‑1 (MQ=255)
                       ttAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGc                                                                           <  2:591018/100‑1 (MQ=255)
                       gtAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGc                                                                           >  1:251579/2‑100 (MQ=255)
                        tAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCt                                                                          >  2:892685/1‑100 (MQ=255)
                         aaCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTg                                                                         >  1:405038/1‑100 (MQ=255)
                         aaCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTg                                                                         >  1:928184/1‑100 (MQ=255)
                          aCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTgg                                                                        <  2:292784/100‑1 (MQ=255)
                          aCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTgg                                                                        <  2:493832/100‑1 (MQ=255)
                           cagcagAGTATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTTTGAACAAGCTgg                                                                        <  1:81063/99‑1 (MQ=255)
                           cagcagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGc                                                                       <  1:1734798/100‑1 (MQ=255)
                           cagcagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGc                                                                       <  1:263308/100‑1 (MQ=255)
                            agcagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGc                                                                       <  1:773754/99‑1 (MQ=255)
                             gcagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGcac                                                                     >  1:1584040/1‑100 (MQ=255)
                               agagAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACaa                                                                   >  1:604634/1‑100 (MQ=255)
                               agagAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACaa                                                                   >  2:713408/1‑100 (MQ=255)
                                gagaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACaaa                                                                  <  2:868192/100‑1 (MQ=255)
                                gagaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACaaa                                                                  <  2:866880/100‑1 (MQ=255)
                                gagaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACaaa                                                                  <  2:866437/100‑1 (MQ=255)
                                  gaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAAtt                                                                <  2:940564/100‑1 (MQ=255)
                                    aTTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGt                                                              <  2:1272771/100‑1 (MQ=255)
                                     ttCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGtt                                                             <  1:1394121/100‑1 (MQ=255)
                                       ccGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTa                                                           <  1:116734/100‑1 (MQ=255)
                                       ccGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTa                                                           <  1:149747/100‑1 (MQ=255)
                                       ccGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTa                                                           <  2:1444546/100‑1 (MQ=255)
                                        cGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTaa                                                          >  1:1350736/1‑100 (MQ=255)
                                         gATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAAt                                                         <  2:642172/100‑1 (MQ=255)
                                            atTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGtt                                                      <  2:1737082/100‑1 (MQ=255)
                                              tAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTa                                                    >  1:823916/1‑100 (MQ=255)
                                               aGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTAc                                                   <  2:1759913/100‑1 (MQ=255)
                                                gATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACa                                                  <  2:1211221/100‑1 (MQ=255)
                                                  tGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACa                                                  >  1:1406914/1‑98 (MQ=255)
                                                   gTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCa                                               >  2:479522/1‑100 (MQ=255)
                                                    tAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaa                                              >  1:1445326/1‑100 (MQ=255)
                                                    tAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATATTTACa                                                  >  1:1922228/1‑96 (MQ=255)
                                                     aaaTATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaaa                                             >  1:532033/1‑100 (MQ=255)
                                                     aaaTATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaaa                                             >  1:1191730/1‑100 (MQ=255)
                                                      aaTATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaaaa                                            <  1:523081/100‑1 (MQ=255)
                                                      aaTATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATAGTTTAATGTTTACAGCaaaa                                            >  2:1720072/1‑100 (MQ=255)
                                                       atatatGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAg                                           <  1:892684/100‑1 (MQ=255)
                                                        tatatGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGa                                          <  2:335129/100‑1 (MQ=255)
                                                        tatatGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGa                                          <  2:149983/100‑1 (MQ=255)
                                                          tatGTCTATCTAGTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATa                                        <  2:1586042/100‑1 (MQ=255)
                                                           atGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATaa                                       >  1:1019458/1‑100 (MQ=255)
                                                             gTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAAcc                                     >  1:930653/1‑100 (MQ=255)
                                                              tctatctaTTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCt                                    >  1:876368/1‑100 (MQ=255)
                                                                 atctatTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCa                                 >  1:207252/1‑100 (MQ=255)
                                                                   ctatTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATg                               >  1:1773808/1‑100 (MQ=255)
                                                                    tatTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATgg                              >  2:1413474/1‑100 (MQ=255)
                                                                     atTTGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgc                             >  2:1423303/1‑100 (MQ=255)
                                                                     atTTGAAAACACTTAAGTGGTTTAGGGCAAATTTACCTAAAAGTGTGAACAAGTTGGCACACATTGTTTAATGTTTACAGCATAAGATAACCTTCATgg                              >  1:1915495/1‑99 (MQ=255)
                                                                      tttGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcg                            <  2:539704/100‑1 (MQ=255)
                                                                      tttGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcg                            >  2:1219964/1‑100 (MQ=255)
                                                                        tGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCa                          >  1:1453608/1‑100 (MQ=255)
                                                                        tGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCa                          >  1:1921173/1‑100 (MQ=255)
                                                                        tGAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCa                          >  1:225247/1‑100 (MQ=255)
                                                                         gAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACGAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGTGCaa                         >  1:1244571/1‑100 (MQ=255)
                                                                           aaaCCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATa                       >  2:100279/1‑100 (MQ=255)
                                                                            aaCCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATaa                      <  2:1019459/100‑1 (MQ=255)
                                                                            aaCCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATaa                      <  2:1584051/100‑1 (MQ=255)
                                                                              cccTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAAcc                    <  2:823917/100‑1 (MQ=255)
                                                                              cccTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAAcc                    <  1:735899/100‑1 (MQ=255)
                                                                              cccTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAAc                     <  1:1271024/99‑1 (MQ=255)
                                                                                  tacgttgttAAGGGTAATTTTACATAAAAGTGTGAACAAGCGGGGACAAATTGTTGAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCCctc                <  2:1622864/97‑1 (MQ=255)
                                                                                  tAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCActc                >  2:1805169/1‑100 (MQ=255)
                                                                                    aGTTGTTAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCtt              >  2:1709510/1‑100 (MQ=255)
                                                                                       tgttAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTc           <  2:690736/100‑1 (MQ=255)
                                                                                        gttAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCg          <  1:677036/100‑1 (MQ=255)
                                                                                        gttAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCg          <  2:1636342/100‑1 (MQ=255)
                                                                                        gttAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCg          >  2:228751/1‑100 (MQ=255)
                                                                                        gttAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCg          >  2:215115/1‑100 (MQ=255)
                                                                                         ttAAGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGc         <  1:713407/100‑1 (MQ=255)
                                                                                         ttAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGc         <  1:1596834/100‑1 (MQ=255)
                                                                                          tAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCt        >  2:968458/1‑100 (MQ=255)
                                                                                           aaGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCtn       <  2:699051/100‑2 (MQ=255)
                                                                                           aaGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTg       <  1:467400/100‑1 (MQ=255)
                                                                                           aaGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTGTACAGCAAAAGATAACCTTACTTGCGCAATAACCACTCTTTTCGCt        >  1:1819723/1‑99 (MQ=255)
                                                                                            aGGGTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGa      >  1:1681012/1‑100 (MQ=255)
                                                                                              ggTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAAc    >  1:1458575/1‑100 (MQ=255)
                                                                                              ggTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAAc    >  1:281401/1‑100 (MQ=255)
                                                                                              ggTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAAc    >  1:1388011/1‑100 (MQ=255)
                                                                                               gTAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACt   >  1:688456/1‑100 (MQ=255)
                                                                                                tAATTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTc  >  2:122354/1‑100 (MQ=255)
                                                                                                   |                                                                                                
GAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTC  >  NC_000913/1399578‑1399773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: