Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,547,395 A→G intergenic (‑226/‑6) yddG ← / → fdnG aromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,547,3950AG94.6% 240.8 / 2.7 74intergenic (‑226/‑6)yddG/fdnGaromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (42/28);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAAG  >  NC_000913/1547298‑1547491
                                                                                                 |                                                                                                
attTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGgaa                                                                                                <  1:425874/100‑1 (MQ=255)
 ttttGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGgaa                                                                                                >  1:912339/1‑99 (MQ=255)
 ttttGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAg                                                                                               >  1:912061/1‑100 (MQ=255)
 ttttGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAg                                                                                               <  1:447080/100‑1 (MQ=255)
  tttGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGc                                                                                              <  2:1797212/100‑1 (MQ=255)
   ttGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATCCCCCTGAAAAAAGAGGGAAGc                                                                                              >  1:554956/1‑99 (MQ=255)
    tgtgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCaa                                                                                            <  2:718615/100‑1 (MQ=255)
    tgtgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCaa                                                                                            >  1:521412/1‑100 (MQ=255)
     gtgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAAt                                                                                           >  2:1890564/1‑100 (MQ=255)
       ggCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATgg                                                                                         <  1:667518/100‑1 (MQ=255)
         cAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGAc                                                                                       >  1:73110/1‑100 (MQ=255)
           aaCAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGt                                                                                     >  2:1261408/1‑100 (MQ=255)
               gATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGt                                                                                 <  2:996882/100‑1 (MQ=255)
               gATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGt                                                                                 >  1:995634/1‑100 (MQ=255)
                aTGTTCTTTTTGATTTCGCGCAAAAAGATCCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTc                                                                                >  1:224101/1‑100 (MQ=255)
                 tGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCATTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCg                                                                               <  1:1065789/100‑1 (MQ=255)
                 tGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCACTGGACGTCAGTCg                                                                               >  2:1438528/1‑100 (MQ=255)
                  gTTCTTTTTGATTTCGCGCATAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGc                                                                              >  1:1190958/1‑100 (MQ=255)
                   ttCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTTAGTCGCa                                                                             <  2:1020036/100‑1 (MQ=255)
                   ttCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCa                                                                             <  2:1542326/100‑1 (MQ=255)
                          tGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtt                                                                      >  1:719568/1‑100 (MQ=255)
                              ttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt                                                                  <  2:208823/100‑1 (MQ=255)
                                cgcgCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaa                                                                <  2:1463669/100‑1 (MQ=255)
                                cgcgCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaa                                                                <  1:1772618/100‑1 (MQ=255)
                                 gcgcAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaaa                                                               <  2:4733/100‑1 (MQ=255)
                                  cgcAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaaaa                                                              <  1:288612/100‑1 (MQ=255)
                                  cgcAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTaaaa                                                              <  1:583133/100‑1 (MQ=255)
                                      aaaaGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTg                                                          >  1:1482540/1‑100 (MQ=255)
                                        aaGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAttttttttttttt                                                           >  1:1595948/1‑90 (MQ=255)
                                           aTTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTgcgc                                                       >  1:442744/1‑98 (MQ=255)
                                           aTTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgg                                                     >  1:1845788/1‑100 (MQ=255)
                                             tCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggc                                                   <  2:479749/100‑1 (MQ=255)
                                              cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                  >  1:1617428/1‑100 (MQ=255)
                                              cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                  <  2:864680/100‑1 (MQ=255)
                                              cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                  >  1:1581209/1‑100 (MQ=255)
                                              cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                  >  1:378695/1‑100 (MQ=255)
                                              cAGAATTTTACTGACAGTTTCCTCGCGAAGAAATACCCCTTAAAAACGAGGGAAGCCAGGGACGTCAGTCGCAGACAATTCTTTAAAATCTGCgcgggcg                                                  >  1:840480/1‑100 (MQ=255)
                                               aGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcgg                                                 <  2:1213593/100‑1 (MQ=255)
                                                 aaTTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTa                                               <  1:1801233/100‑1 (MQ=255)
                                                   ttttACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATg                                             >  2:1437349/1‑100 (MQ=255)
                                                    tttACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATgg                                            >  1:407646/1‑100 (MQ=255)
                                                     ttACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGc                                           >  2:699878/1‑100 (MQ=255)
                                                      tACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCt                                          <  1:1437341/100‑1 (MQ=255)
                                                        cTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTgg                                        >  1:1129567/1‑100 (MQ=255)
                                                          gTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaa                                      >  2:1302040/1‑100 (MQ=255)
                                                          gTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaa                                      >  2:374770/1‑100 (MQ=255)
                                                          gTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaa                                      >  1:223869/1‑100 (MQ=255)
                                                          gTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaa                                      >  1:1695022/1‑100 (MQ=255)
                                                           ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac                                     <  2:653256/100‑1 (MQ=255)
                                                            tAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaaca                                    >  2:809842/1‑100 (MQ=255)
                                                            tAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTAGGGCTGGaa                                      >  2:1757396/1‑98 (MQ=255)
                                                              gTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaacaac                                  >  2:1698444/1‑100 (MQ=255)
                                                                 tCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGt                               <  1:1761614/100‑1 (MQ=255)
                                                                   cTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAg                             >  2:181674/1‑100 (MQ=255)
                                                                   cTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAg                             >  2:166401/1‑100 (MQ=255)
                                                                     cgcgCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCg                           >  1:283857/1‑100 (MQ=255)
                                                                     cgcgCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCg                           <  1:35832/100‑1 (MQ=255)
                                                                      gcgcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCgg                          >  1:992577/1‑100 (MQ=255)
                                                                       cgcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGc                         >  2:52578/1‑100 (MQ=255)
                                                                        gcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCa                        >  1:326054/1‑100 (MQ=255)
                                                                              aTACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGc                  <  1:971226/100‑1 (MQ=255)
                                                                                 ncccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCttt               >  2:369806/2‑100 (MQ=255)
                                                                                 ccccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCttt               >  1:1359980/1‑100 (MQ=255)
                                                                                  cccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAGTCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTg              >  1:74581/1‑100 (MQ=255)
                                                                                  cccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTg              <  1:1901555/100‑1 (MQ=255)
                                                                                   ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc             <  2:36040/100‑1 (MQ=255)
                                                                                   ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc             >  2:389682/1‑100 (MQ=255)
                                                                                   ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc             <  2:878782/100‑1 (MQ=255)
                                                                                   ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc             >  1:190674/1‑100 (MQ=255)
                                                                                    cTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcc            <  2:1581220/100‑1 (MQ=255)
                                                                                     tGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGccc           <  2:1482548/100‑1 (MQ=255)
                                                                                      gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc          >  2:781651/1‑100 (MQ=255)
                                                                                        aaaaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGa        >  1:1839526/1‑100 (MQ=255)
                                                                                          aaaGAGGGAAGCCATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaag      <  1:1608620/100‑1 (MQ=255)
                                                                                          aaaGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaag      <  2:407648/100‑1 (MQ=255)
                                                                                              agGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaagcaag  <  2:194485/100‑1 (MQ=255)
                                                                                                 |                                                                                                
ATTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAAG  >  NC_000913/1547298‑1547491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: