| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,555,324 | A→G | S116P (TCT→CCT) | maeA ← | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,555,324 | 0 | A | G | 95.7% | 229.9 / 0.4 | 70 | S116P (TCT→CCT) | maeA | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (36/31); total (39/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01 | |||||||||||
TTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGT > NC_000913/1555227‑1555419 | gatctattatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaa < 1:1570575/96‑1 (MQ=255) aaTATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaaaa < 1:1502267/100‑1 (MQ=255) attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaaagatc > 2:1570586/1‑96 (MQ=255) attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGc > 1:156550/1‑100 (MQ=255) attatGGTTCGGCACGTTTTGCAGAATATCGGCCATATTGTGAGGATTGGGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaaaa > 1:22280/1‑97 (MQ=255) atGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTca > 1:411085/1‑100 (MQ=255) tGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcac < 2:1868850/100‑1 (MQ=255) ggTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcaca < 1:897345/100‑1 (MQ=255) ggTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcaca > 1:862170/1‑100 (MQ=255) gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAg > 2:1671967/1‑100 (MQ=255) ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg < 1:1510528/100‑1 (MQ=255) gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgc < 1:723150/100‑1 (MQ=255) gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCAGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgc > 1:1367266/1‑100 (MQ=255) gCACGTTTTGCAGAATATCGTCCAGATTGTTCCGGTTCTGGTAAGAGATGAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg < 1:362679/99‑1 (MQ=255) cACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgcg > 2:290101/1‑100 (MQ=255) tttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGAc > 1:1846800/1‑100 (MQ=255) ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACg < 2:71006/100‑1 (MQ=255) tGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACgg > 1:1757793/1‑100 (MQ=255) gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGt > 2:853509/1‑100 (MQ=255) gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGt > 1:1503080/1‑100 (MQ=255) cAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGtt > 1:987486/1‑100 (MQ=255) cAGAATATCGTCCATATTGTGCCGGTTCCGGTAAGAGATACACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGGCGACgac > 1:636829/1‑97 (MQ=255) gAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTgg > 1:1857870/1‑100 (MQ=255) aaTATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTggg < 1:1221892/100‑1 (MQ=255) atatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTgggg > 2:1586144/1‑100 (MQ=255) tatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGt > 2:16489/1‑100 (MQ=255) atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta < 1:1700933/100‑1 (MQ=255) gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataa > 2:1872993/1‑100 (MQ=255) gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataa < 2:1043968/100‑1 (MQ=255) gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCCGTAGATCTCAGGAAAACGATCACAGGCTGCGCCCACGGTTGGGGCataa > 1:81198/1‑100 (MQ=255) cATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaata < 2:1740263/100‑1 (MQ=255) atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACa < 1:1872981/100‑1 (MQ=255) atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACa > 2:1431463/1‑100 (MQ=255) ttGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAg < 2:7985/100‑1 (MQ=255) tgtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAgg < 1:288/100‑1 (MQ=255) tgtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAgg < 2:1094099/100‑1 (MQ=255) gCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcat > 2:189029/1‑100 (MQ=255) ccGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatc > 2:1572197/1‑100 (MQ=255) cGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatca < 1:1892050/100‑1 (MQ=255) cGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatca > 2:1406162/1‑100 (MQ=255) ttCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct < 2:1931693/100‑1 (MQ=255) ttCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct < 1:899713/100‑1 (MQ=255) tCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctc < 1:849423/100‑1 (MQ=255) gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca > 1:101089/1‑100 (MQ=255) tAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCaa > 2:646924/1‑100 (MQ=255) aaGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAg > 1:307874/1‑100 (MQ=255) agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAga > 2:691374/1‑100 (MQ=255) gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat < 1:1572186/100‑1 (MQ=255) gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat > 2:1664895/1‑100 (MQ=255) agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGTCGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatg < 2:524824/100‑1 (MQ=255) agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatg > 1:1797531/1‑100 (MQ=255) gaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatga > 2:479619/1‑100 (MQ=255) gaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatga > 2:21052/1‑100 (MQ=255) aTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatgat < 2:1204100/100‑1 (MQ=255) tAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt > 1:1605419/1‑100 (MQ=255) aaaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg < 1:1428762/100‑1 (MQ=255) acacGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGtt > 2:1594760/1‑100 (MQ=255) cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCGAGATGATTGTTTAc > 1:1321763/1‑100 (MQ=255) cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc > 2:161978/1‑100 (MQ=255) cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc > 2:484724/1‑100 (MQ=255) cgacgcgTGAACGGCGGTAGATCTAAGGAAAACGCTCACAGGCTGCGCAGACGGTTGGGGTATAAATAACAGGCATCATCTCCTCAAGATGATTGTTTaa < 1:285651/97‑2 (MQ=255) gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc < 1:1050460/100‑1 (MQ=255) gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc < 1:122777/100‑1 (MQ=255) ccgaGTGAACGGCGGTAGCTCTAAGGAAAAAGCTCACAGGCTGCGCCGACGGTTCGGGTATAACTAACCGGCCTCATCTCATCAAGCTGATTGTTTAcc < 1:646807/99‑1 (MQ=255) gTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACg < 2:1857882/100‑1 (MQ=255) gAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGt > 2:1069391/1‑100 (MQ=255) cggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga > 2:748033/1‑100 (MQ=255) gcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaag > 1:270864/1‑100 (MQ=255) ggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAagag < 1:1594749/100‑1 (MQ=255) tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg > 1:711390/1‑100 (MQ=255) ctcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg < 1:853508/100‑1 (MQ=255) tcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt < 2:1891457/100‑1 (MQ=255) tcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt > 2:1769186/1‑100 (MQ=255) tcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt < 2:1043308/100‑1 (MQ=255) | TTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGT > NC_000913/1555227‑1555419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |