Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,681,932 T→C P33P (CCA→CCG)  ydgC ← GlpM family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,681,9320TC92.8% 213.5 / 5.5 70P33P (CCA→CCG) ydgCGlpM family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base C (36/28);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

ACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCC  >  NC_000913/1681838‑1682027
                                                                                              |                                                                                               
aCCACATACTAAAAATGGTGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGAGATAAGCGCAAAGGTCGGGAAAAGCGGAAt                                                                                            >  1:1819982/1‑100 (MQ=255)
 ccACATACTAAAAATGATGGTTGCGCATAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATc                                                                                           >  2:1189172/1‑100 (MQ=255)
  cacaTACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGGAAAGCGGAATCa                                                                                          >  1:173452/1‑100 (MQ=255)
        cTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCgg                                                                                    >  1:305864/1‑100 (MQ=255)
         tAAAAATGATGGTTGCGCGTAAGGCTTCAAGGCAGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAGTCAGCCCGGc                                                                                   >  1:1276429/1‑100 (MQ=255)
         tAAAAATGATGGTTGCGCGTAAGGCTTAAATGCCGCGTTCGGTGGCAACAATATAATGCGCGATAAGCGCAAAACTCGAGAAAAGAGAAATCAGcc                                                                                       >  1:5110/1‑96 (MQ=255)
          aaaaaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCg                                                                                  >  1:958323/1‑100 (MQ=255)
            aaaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGat                                                                                >  1:391999/1‑100 (MQ=255)
             aaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGata                                                                               <  1:752263/100‑1 (MQ=255)
             aaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGata                                                                               >  2:755263/1‑100 (MQ=255)
             aaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGata                                                                               >  1:420293/1‑100 (MQ=255)
               tgatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGAtata                                                                             >  2:759568/1‑100 (MQ=255)
                gatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATataa                                                                            >  1:1531527/1‑100 (MQ=255)
                   ggTTGCGCGTAAGGCTTCAGTGCCGCGTTCGGTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGatat                                                                              <  1:1183232/95‑1 (MQ=255)
                    gTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATaataat                                                                        <  2:613639/100‑1 (MQ=255)
                       gcgcGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAAtttt                                                                     >  2:1286094/1‑100 (MQ=255)
                        cgcgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAAttttt                                                                    <  2:1666298/100‑1 (MQ=255)
                         gcgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTc                                                                   <  1:2030/100‑1 (MQ=255)
                          cgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCg                                                                  <  1:1113063/100‑1 (MQ=255)
                            tAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGtt                                                                >  1:33539/1‑100 (MQ=255)
                              aGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGtttt                                                              <  1:1509481/100‑1 (MQ=255)
                              aGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGtttt                                                              <  1:327372/100‑1 (MQ=255)
                              aGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGtttt                                                              >  1:713008/1‑100 (MQ=255)
                               ggCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCCCCGTCCGGGAAAAGCGGAATCAGCCCGCCGAAATAATAATTTTTCGttttt                                                             >  1:1731978/1‑100 (MQ=255)
                               ggCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGttttt                                                             <  2:1121496/100‑1 (MQ=255)
                               ggCTTCAATGCCGCGTTCGCTGGAAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGttttt                                                             <  1:576256/100‑1 (MQ=255)
                                gCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTg                                                            <  1:253947/100‑1 (MQ=255)
                                 cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                           <  1:771548/100‑1 (MQ=255)
                                 cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                           >  2:1201917/1‑100 (MQ=255)
                                 cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                           >  1:591958/1‑100 (MQ=255)
                                 cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                           >  1:1806051/1‑100 (MQ=255)
                                   tCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTa                                                         <  1:593404/100‑1 (MQ=255)
                                   tCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTa                                                         <  2:788435/100‑1 (MQ=255)
                                   tCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTa                                                         >  2:1630440/1‑100 (MQ=255)
                                     aaTGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTaaa                                                       >  1:1564947/1‑100 (MQ=255)
                                     aaTGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTaaa                                                       >  2:1285870/1‑100 (MQ=255)
                                     aaTGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTaaa                                                       <  1:234086/100‑1 (MQ=255)
                                        nccGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAaca                                                    >  2:194184/2‑100 (MQ=255)
                                         ccGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAacac                                                   <  2:1094096/100‑1 (MQ=255)
                                              ttCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATc                                              >  2:916070/1‑100 (MQ=255)
                                               tCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCa                                             <  2:1643862/100‑1 (MQ=255)
                                               tCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCa                                             >  2:1260683/1‑100 (MQ=255)
                                                cGCTGGCAACAATATAATGCGCGATAAGCGAAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATGTAATAATTTTTCGTTTTTGCTAAAACACCAATCaa                                            <  1:388551/100‑1 (MQ=255)
                                                 gCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAAc                                           <  2:1709147/100‑1 (MQ=255)
                                                 gCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAAc                                           >  2:688880/1‑100 (MQ=255)
                                                   tGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAg                                         >  1:1146097/1‑100 (MQ=255)
                                                    ggCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGc                                        <  2:938759/100‑1 (MQ=255)
                                                        acaaTATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTa                                    >  1:1295703/1‑100 (MQ=255)
                                                        acaaTATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTa                                    <  2:844938/100‑1 (MQ=255)
                                                           atatAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTCATAAAACACCAATCAACAGCACTACCa                                 >  1:717970/1‑100 (MQ=255)
                                                              taATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAgcg                              >  1:1298/1‑100 (MQ=255)
                                                              taATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAgcg                              <  2:1564958/100‑1 (MQ=255)
                                                                 tGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCAc                           <  1:758328/100‑1 (MQ=255)
                                                                 tGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCAc                           <  1:1015699/100‑1 (MQ=255)
                                                                     cgATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAg                       >  1:416611/1‑100 (MQ=255)
                                                                          aGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCgg                  >  2:1364642/1‑100 (MQ=255)
                                                                          aGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCgg                  >  1:1838013/1‑100 (MQ=255)
                                                                           ncgcAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTTCTTTATTTTCAATCAACAGAACTACCACGGCAGTGAGCGCgg                  >  1:1622153/2‑99 (MQ=255)
                                                                           gcgcAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGc                 <  1:955573/100‑1 (MQ=255)
                                                                             gcAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCtt               <  2:1518656/100‑1 (MQ=255)
                                                                             gcAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCtt               <  1:30214/100‑1 (MQ=255)
                                                                                 aGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAt           >  2:108510/1‑100 (MQ=255)
                                                                                 aGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAt           <  2:420295/100‑1 (MQ=255)
                                                                                  ggTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAtt          <  2:72776/100‑1 (MQ=255)
                                                                                  ggTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAtt          <  1:417148/100‑1 (MQ=255)
                                                                                   gTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAtt          >  2:1942457/1‑99 (MQ=255)
                                                                                   gTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTa         >  1:931922/1‑100 (MQ=255)
                                                                                        gAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGc    <  1:1910447/100‑1 (MQ=255)
                                                                                        gAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGc    >  2:1073692/1‑100 (MQ=255)
                                                                                          aaaGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGccc  <  2:392001/100‑1 (MQ=255)
                                                                                              |                                                                                               
ACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCCC  >  NC_000913/1681838‑1682027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑