| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 179,327 | A→T | I31F (ATC→TTC) | dgt → | deoxyguanosine triphosphate triphosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 179,327 | 0 | A | T | 95.4% | 219.9 / 1.4 | 65 | I31F (ATC→TTC) | dgt | deoxyguanosine triphosphate triphosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (34/28); total (35/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
AGGACATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCG > NC_000913/179232‑179423 | agGACATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCtt < 2:605606/100‑1 (MQ=255) ggACATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGttcttc > 1:96637/1‑100 (MQ=255) gACATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCg > 2:1557905/1‑100 (MQ=255) cATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCgag < 2:1669891/100‑1 (MQ=255) cATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCgag > 1:1600134/1‑100 (MQ=255) gCACAGATTGATTTCCGAAAAACAATAAACTGGCCTCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGCGATCCTGCGGttcttccaca > 1:1693087/1‑93 (MQ=255) cacaGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGa > 1:927824/1‑100 (MQ=255) caGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATc > 1:896512/1‑100 (MQ=255) aGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATcg > 1:614703/1‑100 (MQ=255) attTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCg < 1:1884647/100‑1 (MQ=255) ccGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatc > 1:526569/1‑100 (MQ=255) ccGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatc > 1:175697/1‑100 (MQ=255) ccGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatc > 2:1150807/1‑100 (MQ=255) ccGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatc > 2:295691/1‑100 (MQ=255) ccGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatc > 1:1031874/1‑100 (MQ=255) cGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatca > 1:406402/1‑100 (MQ=255) aaaaaaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatcatc > 2:271463/1‑100 (MQ=255) aaaaaaaaTAAACTGGCATCGACGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTatcatc > 1:1086371/1‑100 (MQ=255) aaaaaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTAtcatca < 2:1127981/100‑1 (MQ=255) aaaaaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTAtcatca < 2:1139154/100‑1 (MQ=255) aaaaaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTAtcatca > 2:974821/1‑100 (MQ=255) aaaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAAc < 2:284730/100‑1 (MQ=255) aaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAAct > 2:935528/1‑100 (MQ=255) aaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAAct > 2:750504/1‑100 (MQ=255) aaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAAct > 1:1179459/1‑100 (MQ=255) aaaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAAc < 1:1381056/99‑1 (MQ=255) aaaTAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAActc > 2:166635/1‑100 (MQ=255) tAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTcc < 2:1158611/100‑1 (MQ=255) aaCTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCgg > 1:1320202/1‑100 (MQ=255) tGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCaa > 2:1273801/1‑100 (MQ=255) tcgtcgTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATtcgt < 2:1856607/100‑1 (MQ=255) gtcgtTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATtcgtcg < 2:121603/100‑1 (MQ=255) gtcgtTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATtcgtcg > 1:948647/1‑100 (MQ=255) gtTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCt > 2:493044/1‑100 (MQ=255) ttACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTg < 2:948648/100‑1 (MQ=255) tACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGc > 2:41773/1‑100 (MQ=255) ccGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaa < 2:450083/100‑1 (MQ=255) ccGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaa > 1:884750/1‑100 (MQ=255) cGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaac < 1:1350407/100‑1 (MQ=255) gTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaaca > 2:1625654/1‑100 (MQ=255) gTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGcaaca > 2:153145/1‑100 (MQ=255) ccGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGa > 2:1450281/1‑100 (MQ=255) cGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGAc < 1:1150805/100‑1 (MQ=255) gggCGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAg < 1:1598549/100‑1 (MQ=255) cGTTAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGtt < 2:538973/100‑1 (MQ=255) tAAAACCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGttttt > 2:310109/1‑100 (MQ=255) aaaaCCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAGGACCCAGGTTTTTc < 2:884751/100‑1 (MQ=255) aaaaCCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTc < 1:935527/100‑1 (MQ=255) aaaCCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTcc < 2:1356138/100‑1 (MQ=255) aaaCCGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTcc < 2:105738/100‑1 (MQ=255) ccGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACt < 1:1571342/100‑1 (MQ=255) ccGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACt > 1:751542/1‑100 (MQ=255) cGAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTg < 2:654959/100‑1 (MQ=255) cGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTg < 2:191799/100‑1 (MQ=255) gAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTgg > 1:1550704/1‑100 (MQ=255) gAACATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTgg < 2:927825/100‑1 (MQ=255) aCATGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAg < 1:551308/100‑1 (MQ=255) aCATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAg < 1:1344291/100‑1 (MQ=255) tGAGATCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAgcgc < 2:1531161/100‑1 (MQ=255) gagaTCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCa < 2:1332507/100‑1 (MQ=255) gagaTCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCa > 1:1690363/1‑100 (MQ=255) agaTCCTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCaa > 1:1825409/1‑100 (MQ=255) aTCCTGCGGTTCTCCGAGAGCGATCGCGGGCGTATCATAAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATg > 1:1200372/1‑100 (MQ=255) ccTGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATgcc < 1:91131/100‑1 (MQ=255) tGCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATgccgc < 1:1599818/100‑1 (MQ=255) gCGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATgccgcc > 2:403604/1‑100 (MQ=255) cGGTTCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGccgccg > 2:1833017/1‑100 (MQ=255) | AGGACATGGCACAGATTGATTTCCGAAAAAAAATAAACTGGCATCGTCGTTACCGTTCACCGCAGGGCGTTAAAACCGAACATGAGATCCTGCGGATCTTCGAGAGCGATCGCGGGCGTATCATCAACTCTCCGGCAATTCGTCGTCTGCAACAAAAGACCCAGGTTTTTCCACTGGAGCGCAATGCCGCCG > NC_000913/179232‑179423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |