| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,728,444 | C→A | T33N (ACC→AAC) | rnt → | RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,728,444 | 0 | C | A | 95.2% | 210.4 / 2.6 | 62 | T33N (ACC→AAC) | rnt | RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (30/29); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATT > NC_000913/1728352‑1728540 | cGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATgcg > 1:228201/1‑100 (MQ=255) gATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATgcgc < 2:438226/100‑1 (MQ=255) tAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCgctg > 1:649624/1‑100 (MQ=255) aaCGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCgctgc > 1:41462/1‑100 (MQ=255) aaCGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCgctgc < 2:154029/100‑1 (MQ=255) cGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCtt > 1:1149242/1‑100 (MQ=255) gCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTg < 1:542366/100‑1 (MQ=255) gCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTg > 2:462144/1‑100 (MQ=255) gCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATACTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTg > 1:1543130/1‑100 (MQ=255) cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTga < 2:830044/100‑1 (MQ=255) cTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTga > 1:1719868/1‑100 (MQ=255) tCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTgag < 1:1107227/100‑1 (MQ=255) tCAACTTACCGGTCTGTGCGACCGTTTTCGTGGGTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTgag > 2:1030824/1‑100 (MQ=255) cAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTgaga > 1:524727/1‑100 (MQ=255) cAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTgaga > 2:1528603/1‑100 (MQ=255) cAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTgaga < 2:193708/100‑1 (MQ=255) tACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTgcc > 2:880190/1‑100 (MQ=255) aCCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTgccg < 1:868576/100‑1 (MQ=255) cGGTCTGTGCGACCGTTTTCGTGGTTTTGATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTgccgcc < 1:186445/100‑1 (MQ=255) tCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATc > 2:1725020/1‑100 (MQ=255) tgtgCGACCGTTTTCGTGGTTTTTATTTTGTTTTGATGGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCTCCATCAc > 1:1046747/1‑100 (MQ=255) tgtgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCAc < 2:1163278/100‑1 (MQ=255) gtgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCAcc > 2:1053267/1‑100 (MQ=255) tgCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCAccc > 2:839621/1‑100 (MQ=255) ccGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGATGCTTGAGATTGCCGCCATCACCCTGaaa > 1:529074/1‑100 (MQ=255) ttttCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACTATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATg < 1:1662046/100‑1 (MQ=255) ttttCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATg < 1:294937/100‑1 (MQ=255) tCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGAt < 2:1506920/100‑1 (MQ=255) cGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATg < 2:674805/100‑1 (MQ=255) cGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATg < 2:1786385/100‑1 (MQ=255) tGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGaa > 2:1276323/1‑100 (MQ=255) gTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACa < 1:1312482/100‑1 (MQ=255) ccTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggc > 1:213065/1‑100 (MQ=255) ccTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggc < 1:95986/100‑1 (MQ=255) ccTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggc < 2:1719879/100‑1 (MQ=255) cTGTTGTGATCGATGTTGAAACAGCCTGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggct < 1:1790418/100‑1 (MQ=255) cTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAggctggct < 1:977650/100‑1 (MQ=255) gttgtGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGa < 1:308011/100‑1 (MQ=255) ttgtGATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGAt < 1:462142/100‑1 (MQ=255) gATCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCg < 2:1501069/100‑1 (MQ=255) aTCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGGTGAACAAGGCTGGCTGATGCCgg > 1:1400637/1‑100 (MQ=255) aTCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg > 1:676645/1‑100 (MQ=255) aTCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCgg < 2:26606/100‑1 (MQ=255) tCGATGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGa > 1:1829987/1‑100 (MQ=255) tGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcacc > 1:37593/1‑100 (MQ=255) tGTTGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcacc < 1:1613153/100‑1 (MQ=255) ttGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGAcaccac > 2:1379585/1‑100 (MQ=255) tGAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACcaca > 2:332461/1‑100 (MQ=255) gAAACAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacat > 2:299970/1‑100 (MQ=255) aCAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCTGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacattac < 2:1753602/100‑1 (MQ=255) cAGCCGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCacattaca > 1:840156/1‑100 (MQ=255) ccGGATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACAttt > 2:1207858/1‑100 (MQ=255) ggATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTcc > 1:1385796/1‑100 (MQ=255) ggATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTcc < 1:1527362/100‑1 (MQ=255) gATTTAACGTCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCa < 1:1484690/100‑1 (MQ=255) gATTTAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTAATGCCGGACACCACATTACATTTCCa < 1:1331766/100‑1 (MQ=255) ttAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGt < 1:1548707/100‑1 (MQ=255) tAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTc > 2:184930/1‑100 (MQ=255) tAACGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTc > 2:390941/1‑100 (MQ=255) cGCCAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGaa < 2:1258868/100‑1 (MQ=255) gCCAAAAACGATGCGCTTCTTGAGATTGCCGCCAGCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGa < 1:1766620/98‑1 (MQ=255) cAAAAACGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCa > 1:319666/1‑100 (MQ=255) aaaaCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCAtt < 1:1590810/100‑1 (MQ=255) | CGATAACGCTCAACTTACCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTTGTGATCGATGTTGAAACAGCCGGATTTAACGCCAAAACCGATGCGCTGCTTGAGATTGCCGCCATCACCCTGAAAATGGATGAACAAGGCTGGCTGATGCCGGACACCACATTACATTTCCACGTCGAACCATT > NC_000913/1728352‑1728540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |