| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,197,365 | G→A | pseudogene (892/2847 nt) | yehH → | DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,197,365 | 0 | G | A | 94.5% | 231.6 / 3.0 | 73 | pseudogene (892/2847 nt) | yehH | DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (37/32); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTC > NC_000913/2197268‑2197458 | cTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTAcc < 2:1731969/100‑1 (MQ=255) tACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCg < 1:1415206/100‑1 (MQ=255) aCTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGc > 1:1888542/1‑100 (MQ=255) ggAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAAtt > 1:749669/1‑100 (MQ=255) ggAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAAtt < 1:1916111/100‑1 (MQ=255) ggAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAAtt < 1:1913421/100‑1 (MQ=255) gAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGATGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTc > 2:1248623/1‑100 (MQ=255) aGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTcc > 2:1258747/1‑100 (MQ=255) gtaaTATTTTAGATTTGAAAGAGGCCTACGCAAAAATTTATCACTGACGGAATAGACTCTCTGACAAAAATATGCACAAAAACTTATTACAGCAATTCca > 1:1352941/3‑100 (MQ=255) gCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCca > 2:112799/1‑100 (MQ=255) gCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCca < 1:1494040/100‑1 (MQ=255) cAATATTGCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCACAAATGTGCACAAAAACTTATTACCGCAATTCcac > 1:1023033/1‑100 (MQ=255) aaTATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCcaca > 1:1631165/1‑100 (MQ=255) aaTATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCcaca < 2:1053487/100‑1 (MQ=255) aaTATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCcaca < 2:1162508/100‑1 (MQ=255) atTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACatat > 2:604747/1‑100 (MQ=255) ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATAtt > 1:1748966/1‑100 (MQ=255) ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATAtt > 1:657565/1‑100 (MQ=255) cAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCa > 1:1444699/1‑100 (MQ=255) cAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCa > 1:1247468/1‑100 (MQ=255) aTTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGaa > 2:879233/1‑100 (MQ=255) tttGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAAt < 2:379429/100‑1 (MQ=255) ttGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATg > 1:951687/1‑100 (MQ=255) gAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggc < 2:7712/100‑1 (MQ=255) aaTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcg < 2:113862/100‑1 (MQ=255) aaTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcg > 1:1440055/1‑100 (MQ=255) aTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcgg < 2:1383201/100‑1 (MQ=255) aCGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCaa < 1:1503290/100‑1 (MQ=255) ccTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCa < 1:992953/100‑1 (MQ=255) ccTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCa > 1:858294/1‑100 (MQ=255) tACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCAtt > 1:157714/1‑100 (MQ=255) cGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTaa > 1:1033689/1‑100 (MQ=255) gTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAAt > 2:774567/1‑100 (MQ=255) aaaCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTg > 2:41556/1‑100 (MQ=255) ttccgatctcTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCtt < 2:91578/91‑1 (MQ=255) tttATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTg < 2:506077/100‑1 (MQ=255) ttATCACTGATGGAAGAGAATATCTAGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATGTTTCAGAATGGCGGCAACCATTAATTGCTTTGt < 1:1278302/100‑1 (MQ=255) tATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGtt > 2:1644040/1‑100 (MQ=255) tATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGtt < 2:902890/100‑1 (MQ=255) aTCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGtta < 1:218630/100‑1 (MQ=255) tCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGttat < 2:988836/100‑1 (MQ=255) aCTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGttatta > 2:249409/1‑100 (MQ=255) cTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCAAGAATGGAGGCAACCATTAATTGCttagatcggaa > 1:91578/1‑91 (MQ=255) aCGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCg > 2:1249034/1‑100 (MQ=255) gAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGaaa > 2:858685/1‑100 (MQ=255) agTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAAcc < 2:1401602/100‑1 (MQ=255) agTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAAcc < 2:422988/100‑1 (MQ=255) ctctctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGa < 2:1219746/100‑1 (MQ=255) ctctGGCAAAAATGTGCACACAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCCTTAATTGCTTTGTTATTACCCGAAAAACAAGaaa > 1:873174/1‑100 (MQ=255) ctctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGaaa > 1:769199/1‑100 (MQ=255) ctctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCAAATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGaaa > 1:134047/1‑100 (MQ=255) tctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAAt < 2:564688/100‑1 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGca < 1:279189/100‑1 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGca > 1:332879/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac > 1:1489472/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac > 1:593918/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac > 1:1603996/1‑100 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac < 2:1611673/100‑1 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATAATCCAGAATGGCGGCAACCATTAATTGCTTTGAAAAAAAAAAAAAAACCAGAAATTGca < 1:431096/99‑1 (MQ=255) gtgCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAAt < 2:764638/100‑1 (MQ=255) tgCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAAtt > 1:119768/1‑100 (MQ=255) tgCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAAtt > 1:730732/1‑100 (MQ=255) gCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTg > 2:1923992/1‑100 (MQ=255) acaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGcc < 1:518073/100‑1 (MQ=255) caAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGccc < 2:1631175/100‑1 (MQ=255) caAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGccc > 1:393284/1‑100 (MQ=255) caAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGccc > 1:378263/1‑100 (MQ=255) caAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGccc < 2:1263673/100‑1 (MQ=255) caAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGACAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACAGGAAATTGccc > 1:1279806/1‑100 (MQ=255) aaaaCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAg < 2:1497208/100‑1 (MQ=255) aaCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCg < 1:41556/100‑1 (MQ=255) aCTAATTGCCGAACTTCCAAATATTCCAGCCTGGCGGCAAACATTCATTGCTTTGTTATTACCCGCAAACCCAGAACTTGCACCTGAAATTGcaaag < 1:771089/97‑5 (MQ=255) cTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTc < 2:1230360/100‑1 (MQ=255) | CTACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTC > NC_000913/2197268‑2197458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |