| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,235,656 | T→C | I131T (ATC→ACC) | preA → | dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,235,656 | 0 | T | C | 91.2% | 174.3 / 6.1 | 57 | I131T (ATC→ACC) | preA | dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (33/19); total (35/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
ACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGT > NC_000913/2235560‑2235752 | aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa > 2:1430983/1‑100 (MQ=255)aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGa > 2:931603/1‑100 (MQ=255) cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg > 1:1076970/1‑100 (MQ=255) cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg > 1:178288/1‑100 (MQ=255) aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACtt > 1:508610/1‑100 (MQ=255) aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACtt > 1:314628/1‑100 (MQ=255) gTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtct < 2:527851/100‑1 (MQ=255) aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc < 1:1215015/100‑1 (MQ=255) aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc < 1:1213721/100‑1 (MQ=255) ttGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCt < 1:409646/100‑1 (MQ=255) gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGt > 2:1687159/1‑100 (MQ=255) cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTcc < 1:755284/99‑1 (MQ=255) cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg > 1:1582774/1‑100 (MQ=255) cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg > 2:1647843/1‑100 (MQ=255) gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg > 1:1744327/1‑100 (MQ=255) gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg > 1:552393/1‑100 (MQ=255) aTCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGa < 2:1832086/100‑1 (MQ=255) tCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGAc < 2:278086/100‑1 (MQ=255) tCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGAc > 1:782027/1‑100 (MQ=255) gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct > 2:1194682/1‑100 (MQ=255) ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACCTCTCCTGTCCGCAAATGACTtctc < 1:1257947/100‑1 (MQ=255) gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa > 2:1297363/1‑100 (MQ=255) ggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCAt < 1:726753/100‑1 (MQ=255) aaaaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGc > 1:133097/1‑100 (MQ=255) aaaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCTAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg < 1:1194680/100‑1 (MQ=255) aaaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg < 2:1071265/100‑1 (MQ=255) aTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGAt > 1:846233/1‑100 (MQ=255) tGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg > 2:908519/1‑100 (MQ=255) gAGCAGCCATGGGAGGAGCTGGCGCGCCTGGTGCAAGCAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg > 1:920938/1‑100 (MQ=255) gcagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGt > 1:11463/1‑100 (MQ=255) gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc > 2:627922/1‑100 (MQ=255) gaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCg > 2:1654754/1‑100 (MQ=255) gagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc < 2:1065160/100‑1 (MQ=255) gagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc < 1:1713671/100‑1 (MQ=255) cTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaaa > 2:92794/1‑100 (MQ=255) cTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaaa > 1:1430448/1‑100 (MQ=255) tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg > 2:640622/1‑100 (MQ=255) cgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg < 1:1687149/100‑1 (MQ=255) gcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGa > 2:311680/1‑100 (MQ=255) ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg > 2:1000349/1‑100 (MQ=255) tGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg > 2:1482754/1‑99 (MQ=255) tGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt < 1:497548/100‑1 (MQ=255) gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTa < 2:178289/100‑1 (MQ=255) gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTa > 1:999968/1‑100 (MQ=255) agaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaa < 2:551514/100‑1 (MQ=255) aagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaaa > 1:1455069/1‑100 (MQ=255) agCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaaaa < 2:134787/100‑1 (MQ=255) gCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAat > 1:1157679/1‑100 (MQ=255) cTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAata < 2:655651/100‑1 (MQ=255) tGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAatat < 1:1338272/100‑1 (MQ=255) ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt < 2:94450/100‑1 (MQ=255) cgGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCg > 2:343991/1‑100 (MQ=255) gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCgg > 2:84315/1‑99 (MQ=255) tatGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCa > 2:274482/1‑100 (MQ=255) tatGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCa > 2:175140/1‑100 (MQ=255) atGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAg < 2:359997/100‑1 (MQ=255) tGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt > 2:1040273/1‑100 (MQ=255) | ACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGT > NC_000913/2235560‑2235752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |