Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,524,463 Δ1 bp 15.4% intergenic (+95/+18) yncE → / ← ansP ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,524,4630A.15.4% 330.1 / 57.8 117intergenic (+95/+18)yncE/ansPATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base A (51/48);  new base . (10/8);  total (61/56)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 121 total aligned reads displayed.

TGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCA  >  NC_000913/1524364‑1524562
                                                                                                    |                                                                                                   
tGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTa                                                                                                     >  1:120292/1‑100 (MQ=255)
 gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAg                                                                                                    >  1:1136162/1‑100 (MQ=255)
   aaTAGATATGGCGAGGGTGAAACCGGTGAGACTTGCCTGAATTTTATCACGGCGTATCCGGAACTTTCGTCAATAATCTGAATTTGGCCGGCACGTAg                                                                                                    >  2:839752/1‑98 (MQ=255)
    aTAGATATGGCAAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTCTCGTCAGTACTCTGAATTTGCCCGGCACGTAGCCg                                                                                                 >  2:2009918/1‑100 (MQ=255)
      aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                               <  2:2224960/100‑1 (MQ=255)
       gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGc                                                                                              <  1:2339412/100‑1 (MQ=255)
       gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGc                                                                                              <  1:1808140/100‑1 (MQ=255)
       gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAAATTGCCCGGCTACGTGCCGGGc                                                                                              <  2:2315573/100‑1 (MQ=255)
        atatGGCGTTCGTGAAAACGGTCAGACTTTCTTGCCTTTTATTACGGCGTATCCGGCACTTTCGGCAGTAATCTGAATTTGCCCTGCTACGTGCCGGGCa                                                                                             <  2:319752/100‑1 (MQ=255)
        atatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCa                                                                                             <  1:673296/100‑1 (MQ=255)
         tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaa                                                                                            <  1:592608/100‑1 (MQ=255)
         tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCaa                                                                                            >  1:2558931/1‑100 (MQ=255)
            ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAAttt                                                                                         >  1:2212527/1‑100 (MQ=255)
             gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACAGCGTATCCGGCACTTTCGTCAGTAATCTTAAATTGCCCGGCTACGTGCCGGGCAAattc                                                                                        <  2:902309/100‑2 (MQ=37)
              cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                       <  2:1843259/100‑1 (MQ=255)
              cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                       >  2:2375150/1‑100 (MQ=255)
               gAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaaattcag                                                                                      <  2:1945029/100‑7 (MQ=38)
               gAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGc                                                                                      <  1:1432321/100‑1 (MQ=255)
                aGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCa                                                                                     <  1:660173/100‑1 (MQ=255)
                 gtgTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                                    <  2:255879/98‑1 (MQ=255)
                 gggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                                    <  2:876128/100‑1 (MQ=255)
                 gggTCAAAACGTGTACACTTGCCTGCCTTTTATCACGGGGTATCCGGCACTGTCGTCAGTAATCTTAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                                    <  2:1203978/100‑1 (MQ=255)
                  ggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAt                                                                                   >  2:804988/1‑100 (MQ=255)
                  ggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAt                                                                                   >  2:688075/1‑100 (MQ=255)
                     gAAAACGGTGGTACTTGCCTGCGTTTTATCACGGCGTCTCCGGCACTTGCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCAAattc                                                                                        <  2:2022453/92‑2 (MQ=25)
                      aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAAc                                                                               <  1:821476/100‑1 (MQ=255)
                          cGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGc                                                                           >  2:840984/1‑100 (MQ=255)
                          cGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGc                                                                           <  2:79294/100‑1 (MQ=255)
                            gTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCg                                                                         <  1:827734/100‑1 (MQ=255)
                             tGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGt                                                                        <  2:1422289/100‑1 (MQ=255)
                                    tgcctgccTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                                 >  2:813394/1‑100 (MQ=255)
                                    tgcctgccTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                                 <  1:345385/100‑1 (MQ=255)
                                    tgcctgccTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                                 <  1:586153/100‑1 (MQ=255)
                                     gcctgcctTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCtt                                                               <  1:154913/100‑1 (MQ=255)
                                      cctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCtt                                                               >  2:1310397/1‑100 (MQ=255)
                                          cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAac                                                           <  1:138162/100‑1 (MQ=255)
                                           ctTTTATCACGGAGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTGAAaca                                                          >  2:2509321/1‑100 (MQ=255)
                                             tttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAAcaca                                                        <  1:2218952/100‑1 (MQ=255)
                                             tttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAAcaca                                                        <  1:2353744/100‑1 (MQ=255)
                                                aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTATCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTCAACACAAtt                                                     >  2:276205/1‑100 (MQ=255)
                                                aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                     >  1:1484585/1‑100 (MQ=255)
                                                 tCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAttt                                                    >  2:1269986/1‑100 (MQ=255)
                                                   aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                  >  1:1258511/1‑100 (MQ=255)
                                                   aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                  <  1:765755/100‑1 (MQ=255)
                                                   aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                  >  2:1169828/1‑100 (MQ=255)
                                                   aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                                  <  2:1107236/100‑1 (MQ=255)
                                                    cGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCt                                                 <  1:1078831/100‑1 (MQ=255)
                                                        gTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttt                                             <  1:1159407/100‑1 (MQ=255)
                                                         tATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                            <  2:134636/100‑1 (MQ=255)
                                                            ccGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                         <  2:684259/100‑1 (MQ=255)
                                                            ccGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                         <  2:199553/100‑1 (MQ=255)
                                                             cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt                                        <  2:1247645/100‑1 (MQ=255)
                                                              ggCACTTTCGTGAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                       <  2:1716822/100‑1 (MQ=255)
                                                              ggCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                       <  1:2069417/100‑1 (MQ=255)
                                                              ggCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                       >  2:2471787/1‑100 (MQ=255)
                                                               gCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                      <  1:1480022/100‑1 (MQ=255)
                                                                  cTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCt                                   >  2:662607/1‑100 (MQ=255)
                                                                   tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAGCTAGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTGTTGATc                                    <  2:2020712/98‑1 (MQ=255)
                                                                   tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                      <  2:1030237/96‑1 (MQ=255)
                                                                    ttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTc                                 >  2:1593383/1‑100 (MQ=255)
                                                                      cGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt                               <  2:1359964/100‑1 (MQ=255)
                                                                        tCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                             <  1:179995/100‑1 (MQ=255)
                                                                        tCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                             >  2:2376562/1‑100 (MQ=255)
                                                                         cAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcga                            >  2:2488368/1‑100 (MQ=255)
                                                                         cAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcga                            >  1:846292/1‑100 (MQ=255)
                                                                          aGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgac                           >  2:1777644/1‑100 (MQ=255)
                                                                          aGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgac                           >  2:761529/1‑100 (MQ=255)
                                                                              aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                       >  1:765133/1‑100 (MQ=255)
                                                                              aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                       >  1:402590/1‑100 (MQ=255)
                                                                              aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                       >  1:1875618/1‑100 (MQ=255)
                                                                              aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                       >  1:1875676/1‑100 (MQ=255)
                                                                               tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCGTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                       >  2:2018534/1‑99 (MQ=255)
                                                                                ttGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg                     <  1:2099947/99‑1 (MQ=255)
                                                                                 tGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgga                    >  2:2099933/1‑99 (MQ=255)
                                                                                 caaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc                    >  1:2435664/6‑100 (MQ=38)
                                                                                 caaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCGTCCTCGACGACTGgc                    >  1:2522403/6‑100 (MQ=38)
                                                                                 caaaattGCCCGGCTACGTGCCGGGAAATTTTGCAATTAACTCGCCGTTTCAGGCATAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                      >  2:1086463/6‑98 (MQ=37)
                                                                                  gAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcg                   <  2:738719/100‑1 (MQ=255)
                                                                                   aaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCGTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                      >  2:2077825/4‑96 (MQ=38)
                                                                                   aaTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcgc                  >  1:370650/1‑100 (MQ=255)
                                                                                    aattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCa                 >  2:646877/3‑100 (MQ=255)
                                                                                     tttGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAg                <  1:93744/100‑1 (MQ=255)
                                                                                     attGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTTCTGTTTTTCTTCTTCTTCCTCGACGCCTGgcgc                  >  2:1642812/2‑98 (MQ=255)
                                                                                     attGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAg                >  1:1244878/2‑100 (MQ=255)
                                                                                       tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTg              >  1:1007335/1‑100 (MQ=255)
                                                                                       tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTAGACGACTGGCGCAGTg              >  2:2286468/1‑100 (MQ=255)
                                                                                       tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGCTTTTCTTCATCTTCCTCGACGACTGGCGCAGt               >  2:1224485/1‑99 (MQ=255)
                                                                                        gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCGAGCTTAAACACAATTTCCTGCTTTTCTTCATCTTCCTCGACGACTAGCGCAGttc             >  2:1004582/1‑98 (MQ=255)
                                                                                        gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGCCTGGCGCAGTGc             >  1:2275514/1‑100 (MQ=255)
                                                                                        gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGc             >  1:213344/1‑100 (MQ=255)
                                                                                        gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGc             <  2:1950750/100‑1 (MQ=255)
                                                                                         cccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt            >  1:1532042/1‑100 (MQ=255)
                                                                                          ccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt            >  2:1561275/1‑99 (MQ=255)
                                                                                              cACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGaa       >  2:2104258/1‑100 (MQ=255)
                                                                                                 cGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAAtt     <  1:244458/100‑1 (MQ=255)
                                                                                                 cGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAAtt     <  2:846285/100‑1 (MQ=255)
                                                                                                   tAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTc   >  1:500763/1‑100 (MQ=255)
                                                                                                    aGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa  <  1:1255449/100‑1 (MQ=255)
                                                                                                    aGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa  <  1:1521733/100‑1 (MQ=255)
                                                                                                    aGCCGGGCAATTTTGCAATTAACTCGCCGTTTCACGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa  <  2:2432064/100‑1 (MQ=255)
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TGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCA  >  NC_000913/1524364‑1524562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: