Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,712,518 (C)6→5 100% intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,5130C.96.0% 325.8 / 6.5 75intergenic (+355/‑256)nth/dtpADNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base . (43/29);  total (44/31)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

ATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712415‑1712606
                                                                                                  |                                                                                             
atTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcc                                                                                              <  2:632641/100‑1 (MQ=255)
atTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcc                                                                                              <  1:2059464/100‑1 (MQ=255)
  tGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcccc                                                                                            >  1:1881889/1‑100 (MQ=255)
   gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCTCCGTTAAGCCATCAccccc                                                                                           >  2:1604320/1‑100 (MQ=255)
   gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccc                                                                                           >  1:820162/1‑100 (MQ=255)
   gACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccc                                                                                           <  1:2484274/100‑1 (MQ=255)
    aCCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccct                                                                                          <  1:2444767/100‑2 (MQ=255)
      cTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctct                                                                                        >  1:824798/1‑97 (MQ=255)
       tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                       <  1:1204570/100‑5 (MQ=255)
       tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                       >  2:1810399/1‑96 (MQ=255)
       tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                       <  1:1843255/100‑5 (MQ=255)
       tACAAAACATTACACTGGCTATTTTTCAGAAACTGGAATATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATAAccccctctc                                                                                       >  1:349515/1‑96 (MQ=255)
       gaCAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACTCTAAATAGCAGAACATATCGCCGTTAAGCCATCAcaccctc                                                                                         >  2:1807040/2‑96 (MQ=255)
        aCAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTca                                                                                     >  1:2379363/1‑100 (MQ=255)
        aCAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTca                                                                                     >  2:2539336/1‑100 (MQ=255)
          aaaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTATTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagt                                                                                   <  2:1738827/100‑1 (MQ=255)
           aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                  >  1:294666/1‑100 (MQ=255)
             aCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTcagtgc                                                                                 <  1:2530578/100‑1 (MQ=255)
              cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcag                                                                               >  1:36077/1‑100 (MQ=255)
              cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcag                                                                               >  1:1981869/1‑100 (MQ=255)
              cATTACACTGGATATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcag                                                                               <  2:118752/100‑1 (MQ=255)
               aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCAGGAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcagt                                                                              <  2:1285994/100‑1 (MQ=255)
                 tACACTGGCTATTTTTCAGAAACTTGTCTATCTCACTAGTAAAAACGCTAAATAGCAGAACTTCTCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGa                                                                            <  2:481602/100‑1 (MQ=255)
                   cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaa                                                                          <  2:2230180/100‑1 (MQ=255)
                     cTGGCTATTTTTCAGAAACTGGACTATCTCACTGGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaa                                                                        >  2:1533798/1‑100 (MQ=255)
                     cTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaa                                                                        >  1:64189/1‑100 (MQ=255)
                     cTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaa                                                                        >  1:1825675/1‑100 (MQ=255)
                      tGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaaa                                                                       >  1:713472/1‑100 (MQ=255)
                        gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATc                                                                     <  1:386605/100‑1 (MQ=255)
                        gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATc                                                                     <  2:36076/100‑1 (MQ=255)
                           aTTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGc                                                                  <  1:1639383/100‑1 (MQ=255)
                            tttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGcc                                                                 <  1:2365431/100‑1 (MQ=255)
                             ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCg                                                                <  1:1855335/100‑1 (MQ=255)
                             ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCg                                                                <  1:1855448/100‑1 (MQ=255)
                              tttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGc                                                                  >  1:2507077/1‑97 (MQ=255)
                              tttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGt                                                               >  1:1443381/1‑100 (MQ=255)
                               ttCAGAAACTGGACTATCTCACTTGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAACCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGt                                                               >  2:1913642/1‑99 (MQ=255)
                                 cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                            <  1:1005480/100‑1 (MQ=255)
                                  aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                           >  2:888887/1‑100 (MQ=255)
                                  aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                           >  1:481250/1‑100 (MQ=255)
                                  aGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACg                                                           <  1:2248321/100‑1 (MQ=255)
                                   gAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGt                                                          >  1:1604116/1‑100 (MQ=255)
                                    aaaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtt                                                         >  1:1416060/1‑100 (MQ=255)
                                      aCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttt                                                       <  2:1036819/100‑1 (MQ=255)
                                        tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     <  2:970931/100‑1 (MQ=255)
                                            cTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaa                                                 >  2:233387/1‑100 (MQ=255)
                                               tctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAt                                              <  1:2052975/100‑1 (MQ=255)
                                                ctcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTCCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                             <  2:1374820/100‑1 (MQ=255)
                                                ctcACTAGTAAAAACGCTAAATAGCAGAACATAACGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAtt                                             >  2:1534773/1‑100 (MQ=255)
                                                 tcACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAAttt                                            >  1:1369899/1‑100 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  1:722305/1‑100 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  2:1601627/1‑100 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           <  1:1183202/100‑1 (MQ=255)
                                                  cACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTAAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           >  1:1524270/1‑100 (MQ=255)
                                                    cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAc                                         >  1:277456/1‑100 (MQ=255)
                                                      aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGc                                       >  1:199965/1‑100 (MQ=255)
                                                      aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTACAGTGAAAAAATCTGACGTTACGTTTTTTGAAAAATTTAAc                                         >  2:2456799/1‑98 (MQ=255)
                                                      aGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCAGCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGc                                       >  2:1400350/1‑100 (MQ=255)
                                                       gTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGc                                       >  1:1139478/1‑99 (MQ=255)
                                                       gTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCt                                      >  2:1524386/1‑100 (MQ=255)
                                                         aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTgg                                    >  1:1595345/1‑100 (MQ=255)
                                                          aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGa                                   <  2:722298/100‑1 (MQ=255)
                                                           aaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGAt                                  >  2:684744/1‑100 (MQ=255)
                                                             aCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATaa                                >  2:423048/1‑100 (MQ=255)
                                                              cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTTATTTTTTTTACGCTGAATaa                                >  2:1125689/1‑99 (MQ=255)
                                                              cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                               <  2:2371076/100‑1 (MQ=255)
                                                              cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAATGAAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                               <  2:2367904/100‑1 (MQ=255)
                                                                cTAAATAGCAGAACATATCGCAGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                             <  2:713381/100‑1 (MQ=255)
                                                                  aaaTAGCAGAACATATCGCCGTTAAGCCATCACCCCATCTCAGTGCAGTGAAAAAATCTGCCGCTACGTTTTTTTAATAATTTAACACTGGATAACAttt                           >  2:750245/1‑100 (MQ=255)
                                                                   aaTAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAttt                           >  2:624424/1‑99 (MQ=255)
                                                                      aGCATAACATATCGCCGTTAAGCCATTACCCCCTCTCGGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCg                       <  2:739112/100‑1 (MQ=255)
                                                                       gCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCg                       <  1:822478/100‑1 (MQ=255)
                                                                       gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      >  1:2478260/1‑100 (MQ=255)
                                                                       gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      <  2:64188/100‑1 (MQ=255)
                                                                         aGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                              >  2:2239571/1‑90 (MQ=255)
                                                                          gAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTGTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAAt                   >  2:1725469/1‑100 (MQ=255)
                                                                           aaCATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATg                  <  2:1896011/100‑1 (MQ=255)
                                                                             cATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGt                <  2:857451/100‑1 (MQ=255)
                                                                                atCGCCGTTAAGCCATCACCCCCTCTCCGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGCAAAATTTCACGCGGTCTATCATTTCACAGAAAGGTTg              >  2:2289884/1‑99 (MQ=255)
                                                                                atCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa             >  1:1563410/1‑100 (MQ=255)
                                                                                  cGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAAt           >  1:1815473/1‑100 (MQ=255)
                                                                                    ccGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTTTAAATTTTAACGCTGGATAACATTTCCCGGAATGGtcgcat           >  2:1937823/1‑93 (MQ=255)
                                                                                    ccGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTc         <  1:1524397/100‑1 (MQ=255)
                                                                                     cGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcc        >  1:2311719/1‑100 (MQ=255)
                                                                                        atggggaTCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGCTACGTTTTTTGAAAAATTTAACGGTGGATAACATTTCCCGGAATGGTTGAATTcccc      >  2:2014144/7‑100 (MQ=255)
                                                                                          aGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGcc   <  1:1408804/100‑1 (MQ=255)
                                                                                          aGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGcc   <  1:2166429/100‑1 (MQ=255)
                                                                                           gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTCCGTTTTTTGAAAAATTTAACGCTGGATAACATTTCTCGGAATGGTTGAATTCCCCGCCt  >  2:1244297/1‑100 (MQ=255)
                                                                                                  |                                                                                             
ATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712415‑1712606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: