Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,850,715 Δ1 bp 22.6% intergenic (+22/‑145) sppA → / → ansA protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,7150A.22.6% 244.6 / 72.9 93intergenic (+22/‑145)sppA/ansAprotease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base A (43/29);  new base . (8/13);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 8.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

GAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCC  >  NC_000913/1850616‑1850814
                                                                                                   |                                                                                                    
gaaagtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACa                                                                                                      >  1:890981/1‑100 (MQ=255)
ctaagtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACa                                                                                                      <  2:822211/98‑1 (MQ=255)
 aaagtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAg                                                                                                     <  2:1291279/100‑1 (MQ=255)
  aagtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACaag                                                                                                    >  1:822218/1‑98 (MQ=255)
  aagtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGa                                                                                                    >  1:1413233/1‑100 (MQ=255)
   agtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGa                                                                                                    >  2:1640782/1‑99 (MQ=255)
   agtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAt                                                                                                   <  2:278536/100‑1 (MQ=255)
    gtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc                                                                                                  <  1:1995199/100‑1 (MQ=255)
     tgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcg                                                                                                >  1:2459025/1‑100 (MQ=255)
     tgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcg                                                                                                >  1:2458933/1‑100 (MQ=255)
      gaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcgt                                                                                               <  1:2440773/100‑1 (MQ=255)
        cAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcgtcg                                                                                             >  2:1971970/1‑100 (MQ=255)
        cAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcgtcg                                                                                             >  2:820630/1‑100 (MQ=255)
         aaGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCgg                                                                                            >  1:2451112/1‑100 (MQ=255)
           gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGcc                                                                                          <  1:339233/100‑1 (MQ=255)
           gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                          <  1:1639395/100‑1 (MQ=255)
           gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                          <  1:1223040/100‑1 (MQ=255)
           gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                          <  1:586719/100‑1 (MQ=255)
           gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                          <  2:1872245/100‑1 (MQ=255)
            cTGGTCGCGTTTAATGTCCCACAAAACCGTTATGCGTTTTGCCTTACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcg                                                                                          <  2:1306191/99‑2 (MQ=255)
             tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcan                                                                                        <  1:1707610/100‑2 (MQ=255)
             tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                        <  2:1113166/100‑1 (MQ=255)
             tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                        <  1:1653556/100‑1 (MQ=255)
             tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                        <  1:234064/100‑1 (MQ=255)
              ggCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCcaca                                                                                       >  2:1295272/1‑100 (MQ=255)
               gCCGCGTTTAATGGCCCACAATACCGTTATGCGTTTTGCCTGACCTGCGCCCACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                          <  2:2538433/96‑1 (MQ=255)
               gCCGCGTTTAATGCCCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactc                                                                                      <  1:2410689/100‑3 (MQ=255)
               gCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCcaca                                                                                       >  1:1243066/1‑99 (MQ=255)
                 cgcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACatta                                                                                    <  1:1679359/100‑1 (MQ=255)
                 cgcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactca                                                                                     <  2:1772636/99‑4 (MQ=38)
                  gcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACattat                                                                                   <  2:1335606/100‑1 (MQ=255)
                   cgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactcagta                                                                                  <  2:2179683/100‑7 (MQ=38)
                    gTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAttt                                                                                 <  1:822258/100‑1 (MQ=255)
                       tAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAtttttt                                                                              <  1:129235/100‑1 (MQ=255)
                         aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTTAGTGGCCGACAGATCGTCGGCCACATTATTTTTTAc                                                                            <  1:1804010/100‑1 (MQ=255)
                         aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAttttttgc                                                                            >  2:1557820/1‑98 (MQ=255)
                          tGACGCACAAAACCGTTATGCGTTTTGCCTGACGTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTAc                                                                            <  2:2024333/99‑1 (MQ=255)
                           gACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGt                                                                          >  1:1706076/1‑100 (MQ=255)
                            aCCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTc                                                                         >  1:682947/1‑100 (MQ=255)
                             cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                        <  2:71921/100‑1 (MQ=255)
                             cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                        >  1:867021/1‑100 (MQ=255)
                             cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGACACATTATTTTTTACGTc                                                                         >  2:2384573/1‑99 (MQ=255)
                              ccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcga                                                                       >  1:165631/1‑100 (MQ=255)
                               cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                      >  1:1845613/1‑100 (MQ=255)
                               cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                      >  2:3851/1‑100 (MQ=255)
                                acaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgacg                                                                     >  1:2532364/1‑100 (MQ=255)
                                   aaaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAAt                                                                  >  1:2406261/1‑100 (MQ=255)
                                    aaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATc                                                                 <  1:1339751/100‑1 (MQ=255)
                                       cGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctc                                                              >  1:155983/1‑100 (MQ=255)
                                        gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                             >  2:31149/1‑100 (MQ=255)
                                        gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                             >  2:344056/1‑100 (MQ=255)
                                        gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                             <  1:1160937/100‑1 (MQ=255)
                                        gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                             >  1:1363520/1‑100 (MQ=255)
                                          tATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTc                                                           >  2:366860/1‑100 (MQ=255)
                                            tGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTccc                                                         >  2:108197/1‑100 (MQ=255)
                                              cGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGc                                                       <  2:165629/100‑1 (MQ=255)
                                               gTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCt                                                      <  2:1626595/100‑1 (MQ=255)
                                                   tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGttt                                                  >  2:300853/1‑100 (MQ=255)
                                                    gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc                                                 <  1:1766297/100‑1 (MQ=255)
                                                    gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc                                                 >  2:527421/1‑100 (MQ=255)
                                                     ccTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCg                                                >  2:1949821/1‑100 (MQ=255)
                                                        gACCTGCGCCAACATGCGTTAAGTCATGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTGCGccc                                             <  2:1981401/100‑1 (MQ=255)
                                                         aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGcccc                                            <  1:783432/100‑1 (MQ=255)
                                                          ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCa                                           >  1:1627323/1‑100 (MQ=255)
                                                                ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc                                     >  1:2264552/1‑100 (MQ=255)
                                                                 cAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTcc                                    >  1:1316714/1‑100 (MQ=255)
                                                                 cAACATGCGTTAAATCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc                                     >  2:1586971/1‑99 (MQ=255)
                                                                  aaCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCt                                   <  2:1379733/100‑1 (MQ=255)
                                                                    cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCtt                                  >  1:1394980/1‑99 (MQ=255)
                                                                      tGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATc                               >  2:669306/1‑100 (MQ=255)
                                                                        cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCg                             <  1:1290581/100‑1 (MQ=255)
                                                                        cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTGATCCg                             <  2:1505154/100‑1 (MQ=255)
                                                                          ttAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCa                           <  2:672759/100‑1 (MQ=255)
                                                                             aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAg                        <  2:926844/100‑1 (MQ=255)
                                                                              gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGAGGAATCCTCTTCCCGCTGTTTCGACCCATAATTCATTCTcccactatt                       >  2:1810878/1‑93 (MQ=255)
                                                                              gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAg                        >  2:2328354/1‑99 (MQ=255)
                                                                              gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGt                       <  1:1156772/100‑1 (MQ=255)
                                                                                cTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTAt                     >  1:929039/1‑100 (MQ=255)
                                                                                cTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTAt                     <  1:590959/100‑1 (MQ=255)
                                                                                    tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTGCTTATCCGCATAGTAt                     >  2:2134445/1‑96 (MQ=255)
                                                                                    tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg                 >  1:1977331/1‑100 (MQ=255)
                                                                                    tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg                 >  1:2087884/1‑100 (MQ=255)
                                                                                      cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATccg                  >  1:369012/1‑95 (MQ=255)
                                                                                        atgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTCTCCGCATAGTATCAGGTgc              >  2:1899841/3‑99 (MQ=38)
                                                                                        atgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcg             >  1:2512726/3‑100 (MQ=255)
                                                                                         tgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATATTATCAGGTgcgc            >  1:1942693/2‑100 (MQ=255)
                                                                                         tgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcgc            >  1:1431734/2‑100 (MQ=255)
                                                                                         tgTGGCCGACGATCTGTCGGCCACATGATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcgc            >  1:1563084/2‑100 (MQ=255)
                                                                                          gTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt           >  2:677860/1‑100 (MQ=255)
                                                                                          gTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt           >  1:2480596/1‑100 (MQ=255)
                                                                                           tGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTc          >  1:691190/1‑100 (MQ=255)
                                                                                            ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc         >  2:2496750/1‑100 (MQ=255)
                                                                                            ggCCGAAAGATCGTCTGCCACGTCATGACTTACGTCGACGAATCCTCTTCCCGCGGTTTCGCCCCATATTCCATTATCCGCATAGTATCAg                  <  2:646660/91‑1 (MQ=255)
                                                                                             gCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTccc        <  2:1953427/100‑1 (MQ=255)
                                                                                              ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc       >  1:1871179/1‑100 (MQ=255)
                                                                                              ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc       <  2:899298/100‑1 (MQ=255)
                                                                                                   aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGcc  <  2:2451098/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
GAAAGTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCC  >  NC_000913/1850616‑1850814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: