Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,859 Δ1 bp 16.7% intergenic (+17/‑96) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8590C.16.7% 254.8 / 51.6 90intergenic (+17/‑96)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base C (34/41);  new base . (3/12);  total (37/53)
Fisher's exact test for biased strand distribution p-value = 8.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

CCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGA  >  NC_000913/1868761‑1868958
                                                                                                  |                                                                                                    
ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTaca                                                                                                     <  1:1195582/100‑1 (MQ=255)
    atgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATg                                                                                                 <  2:1300373/100‑1 (MQ=255)
    atgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATg                                                                                                 <  1:395647/100‑1 (MQ=255)
    atgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATg                                                                                                 <  1:2308146/100‑1 (MQ=255)
      gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATAGTCTTAACAACCCTGGCACGGTACACATGtt                                                                                              >  2:2357479/1‑100 (MQ=255)
       atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTa                                                                                             >  1:1101654/1‑100 (MQ=255)
        tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACCTGTTAc                                                                                            >  2:69283/1‑100 (MQ=255)
        tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAc                                                                                            >  2:950952/1‑100 (MQ=255)
          gAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                          >  1:2288563/1‑100 (MQ=255)
           aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                         <  2:1506116/100‑1 (MQ=255)
            aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                        <  1:65923/100‑1 (MQ=255)
            aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                        <  1:216220/100‑1 (MQ=255)
            aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                        <  1:2454387/100‑1 (MQ=255)
             aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                       <  1:547027/100‑1 (MQ=255)
              aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcc                                                                                      <  1:2277151/100‑1 (MQ=255)
              aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTAGTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                      <  2:2445982/100‑1 (MQ=255)
               aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                     <  1:2075886/100‑2 (MQ=39)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                    <  1:1380951/100‑3 (MQ=39)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                    <  1:2336955/100‑3 (MQ=39)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                    <  1:1646636/100‑3 (MQ=39)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                    <  1:1755181/100‑3 (MQ=39)
                 gCTTCATCCGGAACCATGTGTGTTTACTTTGCGAATTGGTTGTGCGCGTACGTGAGTCGGCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagg                                                                                   <  2:2332712/100‑4 (MQ=12)
                 gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTAc                                                                                   <  1:2538621/100‑1 (MQ=255)
                 gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagg                                                                                   <  2:922894/100‑4 (MQ=38)
                  cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccaggg                                                                                  <  1:1263343/100‑5 (MQ=38)
                   tACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATAGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                  <  2:165603/99‑1 (MQ=255)
                    acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAAc                                                                                >  1:791870/1‑100 (MQ=255)
                     cacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGGTAACGGGCCTACAAc                                                                                >  2:450552/1‑99 (MQ=255)
                       cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                             <  2:791863/100‑1 (MQ=255)
                         cGTAAACGGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACACTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                           <  2:1575041/100‑1 (MQ=255)
                         cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                           <  2:791025/100‑1 (MQ=255)
                          gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                          <  1:1389853/100‑1 (MQ=255)
                            aaaCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGa                                                                        <  2:1422601/100‑1 (MQ=255)
                              aCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAc                                                                      <  1:385844/100‑1 (MQ=255)
                                 ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                   >  2:380792/1‑100 (MQ=255)
                                  gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                                  <  2:204809/100‑1 (MQ=255)
                                   tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTgg                                                                 <  1:1407712/100‑1 (MQ=255)
                                    gCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAGATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTggg                                                                >  2:593876/1‑100 (MQ=255)
                                    gCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTggg                                                                <  1:1489817/100‑1 (MQ=255)
                                     cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                               <  1:2275761/100‑1 (MQ=255)
                                     cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                               >  1:1508767/1‑100 (MQ=255)
                                      gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGcc                                                              <  1:2460249/100‑1 (MQ=255)
                                         tACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                           >  1:463675/1‑100 (MQ=255)
                                           cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                         >  2:1961435/1‑100 (MQ=255)
                                           cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                         <  1:1939141/100‑1 (MQ=255)
                                           cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                         >  1:2553584/1‑100 (MQ=255)
                                             gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                       >  1:1582147/1‑100 (MQ=255)
                                              tgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGc                                                      <  2:2553568/100‑1 (MQ=255)
                                                cGAATGGTATTTGCGCGTAAGTAAATAGACTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg                                                    >  2:748103/1‑100 (MQ=255)
                                                  aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                  >  1:1976449/1‑100 (MQ=255)
                                                  aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                  >  1:720259/1‑100 (MQ=255)
                                                   aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa                                                 >  1:118950/1‑100 (MQ=255)
                                                       tATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAAcaca                                             >  2:1697694/1‑100 (MQ=255)
                                                        aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                            >  1:1763927/1‑100 (MQ=255)
                                                           tGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                         <  2:2032822/100‑1 (MQ=255)
                                                              gcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAg                                      <  1:2187447/100‑1 (MQ=255)
                                                               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                     >  2:1549327/1‑100 (MQ=255)
                                                                gtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAg                                      >  2:1611687/1‑98 (MQ=255)
                                                                 tacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                   <  1:1910036/100‑1 (MQ=255)
                                                                  acgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATa                                  <  2:2448415/100‑1 (MQ=255)
                                                                   cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                                 >  1:1155878/1‑100 (MQ=255)
                                                                   cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGACTACAAAGACAGCGAACCGGGGGCCTGAGAAGCGGCAACACAGGAGTAGCATAc                                 >  2:610962/1‑100 (MQ=255)
                                                                    gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                                <  1:1447061/100‑1 (MQ=255)
                                                                     taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                               <  1:1654570/100‑1 (MQ=255)
                                                                     taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                               <  1:881152/100‑1 (MQ=255)
                                                                      aaaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGt                              >  2:1815728/1‑100 (MQ=255)
                                                                       aaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt                             >  2:75211/1‑100 (MQ=255)
                                                                        aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                            <  1:2237953/100‑1 (MQ=255)
                                                                        aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCCACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                            <  2:1458609/100‑1 (MQ=255)
                                                                           gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                         <  1:1392739/100‑1 (MQ=255)
                                                                             cTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa                       >  2:1909642/1‑100 (MQ=255)
                                                                               tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATg                     <  1:427584/100‑1 (MQ=255)
                                                                                aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACCACGACAGCGAACCGTGGGCCTGCGAAGCGACACGCCAGGCGCAGCATCCAGCTgcgaaccg                     >  1:2297140/1‑92 (MQ=255)
                                                                                aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                    >  1:743576/1‑100 (MQ=255)
                                                                                aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                    <  2:227848/100‑1 (MQ=255)
                                                                                aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                    <  2:64337/100‑1 (MQ=255)
                                                                                aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                    <  2:336696/100‑1 (MQ=255)
                                                                                  caacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                  <  1:2099201/100‑1 (MQ=255)
                                                                                       cTGGCCCGGTACACGTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg             >  1:1165097/1‑100 (MQ=255)
                                                                                        aggCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg            >  1:1003066/2‑100 (MQ=39)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACACCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCGTACAGTTGGCAAATGTAGTACggggg          >  2:928155/1‑100 (MQ=255)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg          <  1:380796/100‑1 (MQ=255)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg             >  1:302793/1‑97 (MQ=255)
                                                                                          gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg          >  1:1276967/1‑100 (MQ=255)
                                                                                           cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg         <  1:1852586/100‑1 (MQ=255)
                                                                                           cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg         >  1:1214506/1‑100 (MQ=255)
                                                                                            ccGGTACACATGTTACCGGGCCTACAGCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc        >  2:2312300/1‑100 (MQ=255)
                                                                                            ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc        <  2:2258612/100‑1 (MQ=255)
                                                                                            ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc        <  1:1038898/100‑1 (MQ=255)
                                                                                             cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa       >  1:1194116/1‑100 (MQ=255)
                                                                                                tACACATCCTACCGGGCCTACACCGACAGCGAACCGTGCGCCTGAGAAGCGGCCACACAGGCGTAGCATACAGTTGCCAAATGTAGTACGGGGGGCata     <  2:1491725/99‑1 (MQ=255)
                                                                                                  cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAACATACAGTTGGCAAATGTAGTACGGGGGGCATATGa  >  1:1802152/1‑100 (MQ=255)
                                                                                                  |                                                                                                    
CCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGA  >  NC_000913/1868761‑1868958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: