Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,624 Δ1 bp 16.1% coding (426/822 nt) yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6240C.16.1% 326.2 / 61.5 112coding (426/822 nt)yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (47/45);  new base . (9/9);  total (58/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01

Only 100 of 112 total aligned reads displayed.

AGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATG  >  NC_000913/2449526‑2449721
                                                                                                   |                                                                                                 
aGTTTTTACTTACTGACATAATTCGCATTCCATGAGACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                                                                                                  <  1:1472487/100‑1 (MQ=255)
aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                                                                                                  <  2:1462340/100‑1 (MQ=255)
 gTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTTATACGt                                                                                                 >  2:2087525/1‑100 (MQ=255)
  ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                                <  2:579604/100‑1 (MQ=255)
  ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                                <  1:2548551/100‑1 (MQ=255)
  ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                                <  1:1614300/100‑1 (MQ=255)
     tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                                             <  1:849901/100‑1 (MQ=255)
       aCTTACTGACATAATTCTCATTCCATGAAACGTCATACGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGcc                                                                                           >  1:270163/1‑100 (MQ=255)
        cTTACTGACATAATTGTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgaca                                                                                          <  1:1582688/100‑4 (MQ=25)
        cTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgaca                                                                                          <  2:381046/100‑4 (MQ=37)
         ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                         <  1:1511023/100‑5 (MQ=25)
         ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                         <  1:1184045/100‑5 (MQ=25)
          tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGccccc                                                                                        <  2:2522114/100‑1 (MQ=255)
          tACTGACAAAATTCTCATTCCATGAAACGTCATAAGGTTGAAAATAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                         <  2:556537/99‑5 (MQ=21)
           aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCt                                                                                       >  1:2229503/1‑100 (MQ=255)
            cTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg                                                                                      <  1:935663/100‑1 (MQ=255)
              gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTCACTATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                                    <  2:2552084/100‑1 (MQ=255)
               aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacacgatttg                                                                                   <  2:1940078/100‑11 (MQ=21)
                cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                                  >  1:2114237/1‑100 (MQ=255)
                cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACCGACGGACAAATCGTGTCGTGAGTATCAGGCCCCTGATAAGTCACGCCCCCTGgag                                                                                   >  2:1623240/1‑99 (MQ=255)
                 aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                                 <  1:988917/100‑1 (MQ=255)
                  tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAgg                                                                                <  1:2338705/100‑1 (MQ=255)
                     ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                             >  1:1132391/1‑100 (MQ=255)
                     ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                             >  1:1165312/1‑100 (MQ=255)
                       ctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                                           <  2:1701709/100‑1 (MQ=255)
                        tcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                          <  1:2310292/100‑1 (MQ=255)
                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACTTATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                        <  2:627969/100‑1 (MQ=255)
                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                        <  2:901614/100‑1 (MQ=255)
                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                        >  2:225790/1‑100 (MQ=255)
                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                        <  1:895163/100‑1 (MQ=255)
                          aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                          >  1:640378/1‑98 (MQ=255)
                           ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                       >  1:1314239/1‑100 (MQ=255)
                           ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATATGGCGCCTGATACGTCACGCCCCCTGAAGAGCCATCaga                                                                         >  2:801806/1‑98 (MQ=255)
                            tCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGCTACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                       >  2:703993/1‑99 (MQ=255)
                             ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                                     >  1:2250036/1‑100 (MQ=255)
                             ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                                     >  1:1538678/1‑100 (MQ=255)
                             ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                                     >  1:13988/1‑100 (MQ=255)
                              cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                                    >  1:1738622/1‑100 (MQ=255)
                              cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                                    >  2:1687271/1‑100 (MQ=255)
                               aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcatt                                                                   <  1:249851/100‑1 (MQ=255)
                                  aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                <  1:1228467/100‑1 (MQ=255)
                                    aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                                              <  1:225792/100‑1 (MQ=255)
                                      gTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                            <  2:502123/100‑1 (MQ=255)
                                       tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAGTATAAGGCCCCTGATCAGTAACGCCACCTGGAGAGGCTTCACAGTCATCGAtaataa                                                           >  2:758236/1‑100 (MQ=255)
                                         aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                         <  2:446188/100‑1 (MQ=255)
                                         aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                         <  2:447405/100‑1 (MQ=255)
                                             ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                                     >  1:978355/1‑100 (MQ=255)
                                             ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                                     >  1:208585/1‑100 (MQ=255)
                                               ttGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                                   <  2:2090136/100‑1 (MQ=255)
                                                   aCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                               >  2:1303574/1‑100 (MQ=255)
                                                      gAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                                            >  2:1066272/1‑100 (MQ=255)
                                                      gAAACGGATGGACAAATCGGGTCGTTACTATCAGGCCCCTGATACGGCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                                            >  2:2040063/1‑100 (MQ=255)
                                                           ggATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                       <  2:2092835/100‑1 (MQ=255)
                                                            gATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGt                                      <  2:2001881/100‑1 (MQ=255)
                                                             aTGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                                     <  1:580623/100‑1 (MQ=255)
                                                              tGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTaa                                    >  1:2077457/1‑100 (MQ=255)
                                                                gACAAATCGTGTCGTGATTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                  >  1:1572623/1‑100 (MQ=255)
                                                                gACAAATCGTGTCCTGACTATCAGGCCCCTGGTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAGGGACAAGAAAAGTAAGa                                  >  1:1725512/1‑100 (MQ=255)
                                                                 aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                                 >  1:982301/1‑100 (MQ=255)
                                                                 aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                                 <  2:1098496/100‑1 (MQ=255)
                                                                   aaaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGTCCAAGAAAAGTAAg                                   >  1:443008/1‑96 (MQ=255)
                                                                    aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                              >  1:2036447/1‑100 (MQ=255)
                                                                      tcgtgtcgtgACTATCAGGCCCCTGATGCGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAATCCAt                            >  2:113805/1‑100 (MQ=255)
                                                                       gggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                           >  2:450929/6‑100 (MQ=25)
                                                                       gggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                           >  1:630516/6‑100 (MQ=25)
                                                                         gggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtctc                         >  2:366289/4‑100 (MQ=37)
                                                                          gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGACAAGTAAGATTCAATctct                        >  2:2209606/1‑100 (MQ=255)
                                                                          gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGagccgaatca                         >  2:360058/1‑90 (MQ=255)
                                                                          gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct                        >  1:697953/1‑100 (MQ=255)
                                                                          ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct                        >  1:1403256/3‑100 (MQ=37)
                                                                           tcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                       <  1:2020752/100‑1 (MQ=255)
                                                                            cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                      >  1:683753/1‑100 (MQ=38)
                                                                            cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                      >  1:1413950/1‑100 (MQ=38)
                                                                            cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                      >  1:429311/1‑100 (MQ=38)
                                                                            cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                      >  2:867610/1‑100 (MQ=255)
                                                                            cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                      >  2:1090042/1‑100 (MQ=255)
                                                                             gtgacgtATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                     >  1:156223/7‑100 (MQ=38)
                                                                             gtgacgtATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                     <  2:630509/94‑1 (MQ=38)
                                                                             gtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                     >  2:1235169/1‑100 (MQ=255)
                                                                              tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag                    <  1:486285/100‑1 (MQ=255)
                                                                              tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag                    <  1:2136581/100‑1 (MQ=255)
                                                                              tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag                    <  1:1097809/100‑1 (MQ=255)
                                                                              tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag                    <  1:310254/100‑1 (MQ=255)
                                                                              tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                     >  2:464052/1‑99 (MQ=255)
                                                                               gACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga                   >  1:1697477/1‑100 (MQ=255)
                                                                               gACTATCAGGCCCCTGATACCTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga                   <  2:2467262/100‑1 (MQ=255)
                                                                                aCTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                  <  1:606492/100‑1 (MQ=255)
                                                                                aCTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                  <  2:1165304/100‑1 (MQ=255)
                                                                                   tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                <  2:460642/100‑1 (MQ=255)
                                                                                       aGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATa            <  2:443004/100‑1 (MQ=255)
                                                                                        ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATaa           >  1:145700/1‑100 (MQ=255)
                                                                                          ccgcTGATACTTCACTACCCCTGGAGATGCTTCAGAGTCATTCATAATAACGACCCATTGACAAGAGAAGTGAGATTGAATCTCGGCAGAGTCAATAAcg         <  2:2034268/97‑2 (MQ=255)
                                                                                          ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc         >  1:293986/1‑100 (MQ=255)
                                                                                          ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc         <  1:2412908/100‑1 (MQ=255)
                                                                                          ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc         <  1:1804935/100‑1 (MQ=255)
                                                                                            ccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACACGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTc       >  1:212620/1‑100 (MQ=255)
                                                                                                aTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAAt   <  1:196595/100‑1 (MQ=255)
                                                                                                 tACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATg  <  1:155296/100‑1 (MQ=255)
                                                                                                 tACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATg  >  2:8824/1‑100 (MQ=255)
                                                                                                 tACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATg  >  2:2084014/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
AGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATG  >  NC_000913/2449526‑2449721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: