Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,135 (A)6→5 100% coding (9/765 nt) yfeN → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,1300A.95.8% 405.5 / 9.9 96coding (4/765 nt)yfeNputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (39/53);  total (40/56)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GGCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTG  >  NC_000913/2525031‑2525225
                                                                                                   |                                                                                               
ggCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGa                                                                                                 >  2:122627/1‑100 (MQ=255)
 gCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaa                                                                                                >  2:1696004/1‑100 (MQ=255)
  cGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaa                                                                                               >  1:382247/1‑100 (MQ=255)
  cGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaa                                                                                               >  2:2032206/1‑100 (MQ=255)
    atatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaa                                                                                              <  2:1014413/99‑1 (MQ=255)
     tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaac                                                                                            <  2:1155027/100‑2 (MQ=255)
     tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaac                                                                                            >  1:2287916/1‑99 (MQ=255)
        tAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaacatc                                                                                         >  2:1647352/1‑96 (MQ=255)
         aaTTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCt                                                                                       <  2:2131136/100‑1 (MQ=255)
          aTTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCtt                                                                                      >  2:130747/1‑100 (MQ=255)
          aTTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCtt                                                                                      <  2:1944912/100‑1 (MQ=255)
           ttCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCttt                                                                                     <  1:576189/100‑1 (MQ=255)
           ttCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCttt                                                                                     <  1:1024490/100‑1 (MQ=255)
           ttCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCttt                                                                                     <  1:2178385/100‑1 (MQ=255)
           ttCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCttt                                                                                     <  1:1860795/100‑1 (MQ=255)
            tCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCtttt                                                                                    <  1:1051943/100‑1 (MQ=255)
             ccTTTTCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCtttt                                                                                    <  1:683083/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTa                                                                                   <  2:2094203/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTa                                                                                   <  1:456287/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTa                                                                                   <  1:292218/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTa                                                                                   <  2:691059/100‑1 (MQ=255)
             ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGATATGAAAAACATCTTTTa                                                                                   <  2:103738/100‑1 (MQ=255)
              cTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTaa                                                                                  >  1:2175695/1‑100 (MQ=255)
                 tGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAActc                                                                               <  2:423620/100‑1 (MQ=255)
                  gCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAActct                                                                              <  2:1098112/100‑1 (MQ=255)
                   cATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTg                                                                             >  2:1284614/1‑100 (MQ=255)
                    aTTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGa                                                                            >  2:176323/1‑100 (MQ=255)
                    aTTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGa                                                                            <  1:130749/100‑1 (MQ=255)
                     ttAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGa                                                                            <  1:1210689/100‑1 (MQ=255)
                     ttAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGac                                                                           >  1:864635/1‑100 (MQ=255)
                      tAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGaca                                                                          >  2:870085/1‑100 (MQ=255)
                      tAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGaca                                                                          <  2:234498/100‑1 (MQ=255)
                       aaCAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTGATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGacac                                                                         >  1:1334050/1‑100 (MQ=255)
                       aaCAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGacac                                                                         <  2:1761591/100‑1 (MQ=255)
                       aaCAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGacac                                                                         <  1:1686724/100‑1 (MQ=255)
                       aaCAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGacac                                                                         <  1:808474/100‑1 (MQ=255)
                            tatGTCGTTTTTAGCAAGCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTcc                                                                    >  2:2521753/1‑100 (MQ=255)
                            tatGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTcn                                                                    <  1:2231914/100‑2 (MQ=255)
                            tatGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTcc                                                                    <  2:540342/100‑1 (MQ=255)
                              tGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTccac                                                                  >  2:1552048/1‑98 (MQ=255)
                              tGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctc                                                                  <  2:1867633/100‑1 (MQ=255)
                               gTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctct                                                                 >  2:46053/1‑100 (MQ=255)
                               gTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctct                                                                 >  2:907880/1‑100 (MQ=255)
                               gTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctct                                                                 >  1:1445678/1‑100 (MQ=255)
                               gTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctct                                                                 <  1:1371844/100‑1 (MQ=255)
                                  gTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtata                                                              <  1:1423652/100‑1 (MQ=255)
                                  gTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtat                                                               >  1:1959276/1‑99 (MQ=255)
                                    ttttAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATAAAAAACATCTTTTAACTCTGACACTTTCCTCTatat                                                             >  1:2435057/1‑99 (MQ=255)
                                      ttAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAg                                                          <  2:751385/100‑1 (MQ=255)
                                      ttAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAg                                                          >  2:349413/1‑100 (MQ=255)
                                      ttAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAg                                                          <  1:1691390/100‑1 (MQ=255)
                                       tAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGc                                                         <  1:627797/100‑1 (MQ=255)
                                        aGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCg                                                        <  2:536405/100‑1 (MQ=255)
                                         gCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGa                                                       >  2:1254883/1‑100 (MQ=255)
                                          cAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGAt                                                      >  2:2000130/1‑100 (MQ=255)
                                          cAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGAt                                                      >  2:1696863/1‑100 (MQ=255)
                                          cAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGAt                                                      >  2:1072519/1‑100 (MQ=255)
                                          cAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGAt                                                      >  1:2314709/1‑100 (MQ=255)
                                           aaaCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATa                                                     <  2:2212007/100‑1 (MQ=255)
                                            aaCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATAc                                                    <  2:693252/100‑1 (MQ=255)
                                              ccACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCt                                                  >  2:514989/1‑100 (MQ=255)
                                               cACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtg                                                 >  2:400142/1‑100 (MQ=255)
                                                aCTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgt                                                >  2:254843/1‑100 (MQ=255)
                                                  tttATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTACCTCTGACACTTTCCTCTATATTAGCGATACCtgttg                                              <  2:1338912/100‑1 (MQ=255)
                                                  tttATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgttg                                              <  2:2320832/100‑1 (MQ=255)
                                                   ttattaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgttgt                                             <  1:2373749/100‑1 (MQ=255)
                                                      ttaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtc                                          <  1:2446669/100‑1 (MQ=255)
                                                        aaGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtctc                                        >  1:2220131/1‑100 (MQ=255)
                                                         aGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCa                                       <  1:959388/100‑1 (MQ=255)
                                                         aGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCa                                       <  1:1587342/100‑1 (MQ=255)
                                                           cAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATg                                     >  2:274736/1‑100 (MQ=255)
                                                           cAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATg                                     >  1:1421787/1‑100 (MQ=255)
                                                           cAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATg                                     >  2:2495796/1‑100 (MQ=255)
                                                             aTCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCa                                   <  2:58409/100‑1 (MQ=255)
                                                               ctactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGa                                 <  2:297365/100‑1 (MQ=255)
                                                                tactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGa                                 >  2:408291/1‑99 (MQ=255)
                                                                tactaATAACGATCGACATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGaa                                >  1:966365/1‑100 (MQ=255)
                                                                 actaATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGaa                                <  1:1815935/100‑1 (MQ=255)
                                                                         cGATCGGCATAGGGAATTATGCTATGAAAAACATTTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAgg                        >  2:390275/1‑99 (MQ=255)
                                                                         cGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGc                       <  1:223067/100‑1 (MQ=255)
                                                                         cGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGc                       <  2:302666/100‑1 (MQ=255)
                                                                            tCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGt                    <  2:1701355/100‑1 (MQ=255)
                                                                             cGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGtt                   <  2:1182243/100‑1 (MQ=255)
                                                                             cGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGtt                   <  1:2000144/100‑1 (MQ=255)
                                                                              ggCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGttt                  >  1:342335/1‑100 (MQ=255)
                                                                               gCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGtttt                 >  1:2320486/1‑100 (MQ=255)
                                                                                cATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTg                <  2:560581/100‑1 (MQ=255)
                                                                                cATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTg                <  2:2130196/100‑1 (MQ=255)
                                                                                cATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTg                >  2:2416075/1‑100 (MQ=255)
                                                                                 aTAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGc               <  1:1402498/100‑1 (MQ=255)
                                                                                  tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa              <  2:1410642/100‑1 (MQ=255)
                                                                                  tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa              <  1:1338146/100‑1 (MQ=255)
                                                                                  tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATAGTAGCGATACCTGTGGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa              <  2:630638/100‑1 (MQ=255)
                                                                                   aGGGAATTATGCTATGAAAAGCATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCCTGCAGAATTTAAAGGAGGTTTTGCAg             >  2:1564151/1‑100 (MQ=255)
                                                                                     ggAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAACGCGGTTTTGAAGat           >  2:1169265/1‑100 (MQ=255)
                                                                                      gAATTATGCGATGAAAAACATCTTTTAACTCTTACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGata          <  1:384986/100‑1 (MQ=255)
                                                                                          tATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCgg      >  1:454978/1‑100 (MQ=255)
                                                                                           aTGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGc     >  1:2043719/1‑100 (MQ=255)
                                                                                             gCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGt   <  1:749815/100‑1 (MQ=255)
                                                                                              cTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTg  >  1:1764212/1‑100 (MQ=255)
                                                                                                   |                                                                                               
GGCGATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGTG  >  NC_000913/2525031‑2525225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: