Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,350 Δ1 bp 17.4% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3500A.17.4% 311.6 / 66.3 115intergenic (+27/+463)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (55/40);  new base . (12/8);  total (67/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 116 total aligned reads displayed.

CTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTG  >  NC_000913/3183251‑3183448
                                                                                                    |                                                                                                  
cTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTACAGCGCa                                                                                                    >  2:419963/1‑100 (MQ=255)
 tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                   >  2:397665/1‑100 (MQ=255)
 tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                   >  1:443883/1‑100 (MQ=255)
  aTGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATg                                                                                                  >  1:1840581/1‑100 (MQ=255)
   tGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                                 <  1:2282497/100‑1 (MQ=255)
     gCGTACTATGTGGGATGTCAGTGATGGGATTAAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                               >  1:1725567/1‑100 (MQ=255)
     gCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                               >  1:865684/1‑100 (MQ=255)
      cGTACTATGTGGAATGTCAGTGATGGGATTCATTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACa                                                                                              <  2:23660/100‑1 (MQ=255)
          cTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACTACATCAGAAGTTGTCCATGCGCTGGACAACtt                                                                                          <  1:2429855/100‑1 (MQ=38)
           tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAAGTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                         <  2:1078928/100‑1 (MQ=38)
            aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                         <  1:890047/99‑1 (MQ=38)
            aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACtt                                                                                          >  2:2204222/1‑98 (MQ=255)
            aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTc                                                                                         <  2:1995837/99‑1 (MQ=255)
              gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                      >  2:69911/1‑100 (MQ=255)
              gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                      <  1:1849572/100‑1 (MQ=255)
              gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                      >  1:1030819/1‑100 (MQ=255)
               tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAGCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgat                                                                                     <  2:2035783/100‑3 (MQ=37)
               tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACAGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTGCTGcc                                                                                     <  2:304408/100‑1 (MQ=255)
               tgGAATGTATGTGATGGGATTCAGTTAAGTCCTGCTACAAGTGGTGGGGATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACACCTTCTgat                                                                                     <  2:2056275/100‑3 (MQ=19)
                ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                    >  1:568231/1‑100 (MQ=255)
                ggAACGTCAGTGATGGGATGCAGTGTAGTGCTGCTACAAAGGGAAGGAAAAAATTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatg                                                                                    <  2:2493897/100‑4 (MQ=12)
                 gAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgt                                                                                   <  2:2349316/100‑5 (MQ=37)
                    tGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACACCATCAGAAGTTGTCCAGCGCATGGAAAACTTCTGCCAGCtt                                                                                >  2:2122364/1‑100 (MQ=255)
                      tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                              >  2:1268767/1‑100 (MQ=255)
                      tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                              >  2:904654/1‑100 (MQ=255)
                      tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                              >  2:2560501/1‑100 (MQ=255)
                       cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                             >  2:898426/1‑100 (MQ=255)
                       cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                             >  1:874230/1‑100 (MQ=255)
                        gatcttGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACg                                                                            <  2:181532/95‑1 (MQ=255)
                             tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGagatc                                                                       >  1:181534/1‑96 (MQ=255)
                              gggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGcc                                                                      <  2:2414295/100‑1 (MQ=255)
                                gATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                    >  1:483647/1‑100 (MQ=255)
                                  ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                                  <  1:2301585/100‑1 (MQ=255)
                                  ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                                  >  1:2245159/1‑100 (MQ=255)
                                  ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                                  <  2:568224/100‑1 (MQ=255)
                                   tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                                 <  1:2504376/100‑1 (MQ=255)
                                    ctgTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                <  2:1406475/98‑1 (MQ=255)
                                    cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                <  1:853161/100‑1 (MQ=255)
                                    cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                <  2:865187/100‑1 (MQ=255)
                                    cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                >  1:1649589/1‑100 (MQ=255)
                                      gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                              >  1:2286629/1‑100 (MQ=255)
                                        ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                            >  1:1443032/1‑100 (MQ=255)
                                        ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                            <  2:1597657/100‑1 (MQ=255)
                                        ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                            <  2:1319377/100‑1 (MQ=255)
                                         tAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCt                                                           <  1:1471303/100‑1 (MQ=255)
                                              ctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                      <  1:2494048/100‑1 (MQ=255)
                                                gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                    >  1:1006727/1‑100 (MQ=255)
                                                gctACAAACGGCGGGAAATGGTTAAAAACACCATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                    <  2:1461351/100‑1 (MQ=255)
                                                 ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                                   >  2:314499/1‑100 (MQ=255)
                                                 ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                                   >  1:2509612/1‑100 (MQ=255)
                                                 ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                                   <  2:1820729/100‑1 (MQ=255)
                                                 ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                                   >  1:3188/1‑100 (MQ=255)
                                                   aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                  >  1:703874/1‑99 (MQ=255)
                                                    cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCGGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtc                                                >  1:620918/1‑100 (MQ=255)
                                                    cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtc                                                <  1:371237/100‑1 (MQ=255)
                                                      aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTTCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                               >  2:2263246/1‑99 (MQ=255)
                                                      aaCGGCGGCAATTGGTTAAAAACAACAGCCGAAGTTGTGCAGCTTATGGACAACTTCTGCCAGCTGCACGAAATCCTTATTCTACCTTTTCGGCGGtct                                               <  2:308768/99‑1 (MQ=255)
                                                         ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                           <  2:2509597/100‑1 (MQ=255)
                                                         ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                           <  1:925550/100‑1 (MQ=255)
                                                         ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                           >  2:816026/1‑100 (MQ=255)
                                                          gcggTAATTGGTTAAAAACAACATCAGAAGTTGTCCCGCGCATGGACAACTTCTGCCTGCTTCACGAAAGCCTTATTCTACCGTTTCGGCTGtctcttc                                           <  2:721780/99‑1 (MQ=255)
                                                          gcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                          >  2:1114882/1‑100 (MQ=255)
                                                           cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                         <  2:1011218/100‑1 (MQ=255)
                                                               aaTTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGt                                     >  1:658155/1‑100 (MQ=255)
                                                                   ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                 >  2:1966592/1‑100 (MQ=255)
                                                                   ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                 >  2:2454539/1‑100 (MQ=255)
                                                                    gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                >  1:461759/1‑100 (MQ=255)
                                                                    gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                >  1:1643859/1‑100 (MQ=255)
                                                                     ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                               >  1:1141868/1‑100 (MQ=255)
                                                                      tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                              <  1:395300/100‑1 (MQ=255)
                                                                      tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                              >  2:716791/1‑100 (MQ=255)
                                                                      tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                              <  2:687321/100‑1 (MQ=255)
                                                                      tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                              >  2:838790/1‑100 (MQ=255)
                                                                            caacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                        >  2:392659/1‑100 (MQ=255)
                                                                            atctggcAGAAGTCGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                        >  1:1739915/7‑100 (MQ=37)
                                                                               caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                     <  1:1356988/100‑1 (MQ=255)
                                                                               caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                     >  2:1040978/1‑100 (MQ=255)
                                                                                ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                    >  1:1630488/3‑100 (MQ=38)
                                                                                ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                    >  1:958263/3‑100 (MQ=38)
                                                                                aTCAGAAGTTGTCCAGCGCATTGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                    <  2:1916719/100‑1 (MQ=255)
                                                                                aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                    >  2:886981/1‑100 (MQ=255)
                                                                                aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                    >  1:1515822/1‑100 (MQ=255)
                                                                                aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                    >  1:1183755/1‑100 (MQ=255)
                                                                                  cAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc                  >  1:265561/1‑100 (MQ=38)
                                                                                   aGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                 >  1:1537305/1‑100 (MQ=38)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                >  2:385615/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                >  2:471160/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                >  1:2551069/1‑100 (MQ=39)
                                                                                    gAAGTTGTCCATGCGATGGACAACTTCTGCCAGCTGCACGAAAGCCTTATTCGACCTTTTCGGCTGTATATTCCCTGGTAATGGTTCAGTCTTTGCTct                 >  2:44608/1‑98 (MQ=16)
                                                                                    gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                >  2:560486/1‑100 (MQ=255)
                                                                                      aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg              >  1:100512/1‑100 (MQ=39)
                                                                                      aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc               >  2:2092644/1‑99 (MQ=39)
                                                                                       gctttCCAGCGCATGGACACCTTCTGGCAGCTTCCCTAAAGCTTTATGCTACCTTTTCGGCTGTCTCTGCTCTCGTCCGGTTTAAGTATTTGTTCcgc               <  2:492358/94‑1 (MQ=255)
                                                                                        ttGTCCAGCGCATGGACAACTTCTGCCAGCTTCTCGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg              <  2:1237266/98‑1 (MQ=255)
                                                                                          gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc          >  1:1980861/1‑100 (MQ=255)
                                                                                             cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAg       >  1:2123226/1‑100 (MQ=255)
                                                                                             cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAg       <  2:283557/100‑1 (MQ=255)
                                                                                              aGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc      <  2:1887882/100‑1 (MQ=255)
                                                                                                   cATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTg  <  1:1294431/100‑1 (MQ=255)
                                                                                                   cATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTg  >  2:488884/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
CTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTG  >  NC_000913/3183251‑3183448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: