Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,927,735 Δ1 bp 100% coding (581/993 nt) asnA → asparagine synthetase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,927,7340G.98.0% 459.5 / 0.6 102coding (580/993 nt)asnAasparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (51/49);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCA  >  NC_000913/3927635‑3927829
                                                                                                   |                                                                                               
cTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTg                                                                                                 >  1:115853/1‑100 (MQ=255)
cTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTg                                                                                                 <  2:346926/100‑1 (MQ=255)
cTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTg                                                                                                 <  1:2059864/100‑1 (MQ=255)
 tGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgc                                                                                                >  2:274261/1‑99 (MQ=255)
  gCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcg                                                                                               >  1:1107389/1‑98 (MQ=255)
  gCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcg                                                                                               >  1:2353562/1‑98 (MQ=255)
  gCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcg                                                                                               >  1:524558/1‑98 (MQ=255)
  gCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGACAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcg                                                                                               <  2:1370926/100‑3 (MQ=255)
   ccGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAACGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcgc                                                                                              >  2:1350001/1‑97 (MQ=255)
   ccGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcgn                                                                                              <  1:2020233/100‑4 (MQ=255)
    cGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcgcg                                                                                             >  1:682878/1‑96 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCgg                                                                                           <  2:115056/100‑1 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCgg                                                                                           >  1:2453075/1‑100 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCATGAGCGGGCGATAGCGAAAGATCTT‑GCGCgg                                                                                           <  2:2298318/100‑1 (MQ=255)
     ggAGCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCgg                                                                                           <  1:2102687/100‑1 (MQ=255)
      gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGt                                                                                          >  2:176837/1‑100 (MQ=255)
      gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGt                                                                                          >  2:1935117/1‑100 (MQ=255)
          aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTc                                                                                      >  1:701658/1‑100 (MQ=255)
           tccgatcTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTcc                                                                                     <  1:1607196/94‑1 (MQ=255)
           gATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTcc                                                                                     <  1:1650554/100‑1 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTAGTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGt                                                                                 <  1:280981/100‑1 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGt                                                                                 <  1:401034/100‑1 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGt                                                                                 >  2:1913680/1‑100 (MQ=255)
                aCTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTc                                                                                <  2:2220837/100‑1 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                              <  1:2038979/100‑1 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                              <  2:1847285/100‑1 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                              <  2:811580/100‑1 (MQ=255)
                   tCGTCCACAGCCAGGAGTTACTGTCCCGTTATCCGGAGCTTGATGCCAAAAGGCGTGTGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCGTGTCggg                                                                             >  2:1480339/1‑100 (MQ=255)
                   tCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGCGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCg                                                                               >  2:948735/1‑98 (MQ=255)
                   tCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTccagatcgga                                                                             >  2:1607185/1‑92 (MQ=255)
                   tCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCggg                                                                             <  1:2271551/100‑1 (MQ=255)
                   tCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                              >  2:440211/1‑99 (MQ=255)
                   tCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGAGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                              >  2:562688/1‑99 (MQ=255)
                    cGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGa                                                                            >  1:288774/1‑100 (MQ=255)
                     gTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGAt                                                                           <  1:1685521/100‑1 (MQ=255)
                        cacaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTgg                                                                        <  2:275809/100‑1 (MQ=255)
                        cacaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTgg                                                                        <  1:1628684/100‑1 (MQ=255)
                        cacaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCGT‑GCGCGGTATTCCTTGTCGGGGTTgg                                                                        >  2:2077838/1‑100 (MQ=255)
                        cacaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCAGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTgg                                                                        <  1:54147/100‑1 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                      <  1:1501001/100‑1 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                      >  2:1695697/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcggt                                                                    >  2:1503504/1‑99 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcggc                                                                    >  2:2022140/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcggc                                                                    >  1:471466/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcggc                                                                    >  1:523199/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTAACCGGATCTTGATGCCGAGGGGCGTGCGCGGGCCATAGCTAAAGATCGT‑GCGCGATATTCCTTGTCGAGATTggc                                                                       <  2:1138048/97‑1 (MQ=255)
                             ccAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCa                                                                   <  2:2439616/100‑1 (MQ=255)
                                ggAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCAGCaa                                                                  >  2:1641309/1‑99 (MQ=255)
                                ggAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGc                                                                >  2:2319738/1‑100 (MQ=255)
                                ggAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGc                                                                <  1:1238021/100‑1 (MQ=255)
                                ggAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGc                                                                >  1:991746/1‑100 (MQ=255)
                                   gTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGCTTGGCGGCAAGCTGa                                                             >  1:373075/1‑100 (MQ=255)
                                   gTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTg                                                              >  1:2099337/1‑99 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCCAGCTGAGc                                                           >  1:759495/1‑100 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                           <  1:104315/100‑1 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                           <  1:2052302/100‑1 (MQ=255)
                                      aCTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCg                                                          >  1:1370683/1‑100 (MQ=255)
                                      aCTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCg                                                          >  2:1898325/1‑100 (MQ=255)
                                       cTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                         >  2:364181/1‑100 (MQ=255)
                                         gTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTGGGGATTGGCGGCAAGCTGAGCGATg                                                       >  1:514924/1‑100 (MQ=255)
                                          tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                      <  2:854735/100‑1 (MQ=255)
                                          tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                      <  1:1795104/100‑1 (MQ=255)
                                           ctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGt                                                     >  2:756331/1‑100 (MQ=255)
                                           ctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGt                                                     <  2:1947305/100‑1 (MQ=255)
                                            tcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTc                                                    >  1:1760534/1‑100 (MQ=255)
                                             cGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCa                                                   <  2:1129659/100‑1 (MQ=255)
                                                tATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCg                                                <  2:613915/100‑1 (MQ=255)
                                                 aTCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGc                                               >  2:1112539/1‑100 (MQ=255)
                                                   ccGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGACCATCGctc                                             <  1:1428949/100‑3 (MQ=255)
                                                    cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCac                                            >  1:410501/1‑100 (MQ=255)
                                                     ggATATTGATGCCTAAGTTCTTGAGCGTGCGATAGCGAAAGATCTT‑GCGCGGTATTTCTTGTCGGGATTGGTGGCAAGCTGAGTGATGGTCATAGCCac                                            <  2:249899/99‑1 (MQ=255)
                                                       aTCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgac                                         >  2:797915/1‑100 (MQ=255)
                                                          ttGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTg                                      >  1:991749/1‑100 (MQ=255)
                                                              tGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcg                                    >  2:798517/1‑98 (MQ=255)
                                                                 cAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCAc                               >  1:455215/1‑100 (MQ=255)
                                                                   aaGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCg                             <  1:322514/100‑1 (MQ=255)
                                                                   aaGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCg                             >  1:132216/1‑100 (MQ=255)
                                                                    aGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCgg                            <  1:1692123/100‑1 (MQ=255)
                                                                    aGGGCGTGAGCGGGCGATAGCGAAAGACCGT‑GCGCTGTATTCCTTGTCGGGATTGGCGGGAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCgg                            <  1:1222108/100‑1 (MQ=255)
                                                                      ggCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAt                          >  1:2363027/1‑100 (MQ=255)
                                                                      agcatGATCGTGCCATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAt                          <  1:472177/96‑1 (MQ=255)
                                                                       gCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAtt                         <  2:288770/100‑1 (MQ=255)
                                                                          tGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatg                      >  1:1673077/1‑100 (MQ=255)
                                                                          tGAGCGGGCGACAGCGATATAGC‑TGCCGTGGTACCCCGCGTGTGGATTGGCGGCAAGCTGTGGGATGGTGGTCGCCAAGACGTGCGCGCACTGGac                          <  2:2526307/96‑2 (MQ=255)
                                                                            atctggCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                    <  1:1384182/96‑1 (MQ=255)
                                                                             gCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgatg                   <  1:2387212/100‑1 (MQ=255)
                                                                                 gCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg               >  1:2039729/1‑100 (MQ=255)
                                                                                 gCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg               >  1:963383/1‑100 (MQ=255)
                                                                                  cGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTgg              <  1:1412955/100‑1 (MQ=255)
                                                                                   gATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGa             <  1:1435362/100‑1 (MQ=255)
                                                                                    aTAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAg            >  1:1329459/1‑100 (MQ=255)
                                                                                     tAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAg            <  2:1111181/99‑1 (MQ=255)
                                                                                       gCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAc         >  2:216864/1‑100 (MQ=255)
                                                                                       gCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAc         >  2:1240825/1‑100 (MQ=255)
                                                                                       gCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGAGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAc         >  2:1369245/1‑100 (MQ=255)
                                                                                        cGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCGCGACGTGCGCGCACCGGATTATGATGCCTGGAGCa          >  1:189588/1‑98 (MQ=255)
                                                                                        cGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc        >  2:2109610/1‑100 (MQ=255)
                                                                                        cGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc        >  2:1067271/1‑100 (MQ=255)
                                                                                             gATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTc   <  1:1101296/100‑1 (MQ=255)
                                                                                              aTCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCa  <  1:2227688/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCA  >  NC_000913/3927635‑3927829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑