Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 949,741 Δ1 bp 15.1% coding (74/591 nt) ycaK → putative NAD(P)H‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913949,7410C.15.1% 270.2 / 46.9 93coding (74/591 nt)ycaKputative NAD(P)H‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (40/38);  new base . (9/5);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

CCAGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGA  >  NC_000913/949642‑949840
                                                                                                   |                                                                                                    
ncAGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGc                                                                                                      >  2:1300114/2‑100 (MQ=255)
ccAGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGc                                                                                                      <  2:839470/100‑1 (MQ=255)
ccAGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGc                                                                                                      >  2:418825/1‑100 (MQ=255)
 cAGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGc                                                                                                      <  2:2174410/99‑1 (MQ=255)
  aGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGa                                                                                                    >  1:114170/1‑100 (MQ=255)
   gAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGAt                                                                                                   <  1:2381838/100‑1 (MQ=255)
    aaCTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATc                                                                                                  <  1:2156766/100‑1 (MQ=255)
    aaCTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATc                                                                                                  <  2:1865951/100‑1 (MQ=255)
      cTTAACGTGGAGGTAAAATTATGCAGTCTGATCGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCa                                                                                               <  2:1179275/100‑1 (MQ=255)
      cTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCa                                                                                               <  2:1962356/100‑1 (MQ=255)
      cTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCa                                                                                               >  2:1234556/1‑100 (MQ=255)
        tAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATc                                                                                             <  1:1293156/100‑1 (MQ=255)
          aCGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAg                                                                                           >  1:56347/1‑100 (MQ=255)
           cGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGc                                                                                          >  1:403934/1‑100 (MQ=255)
            gTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGca                                                                                         <  2:495415/100‑2 (MQ=255)
             tGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaa                                                                                        <  1:2559255/100‑3 (MQ=255)
             tGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaa                                                                                        <  1:2335227/100‑3 (MQ=255)
               gAGGTAAAATTATGCAGTCTGAACGTATTTATGTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGc                                                                                      <  2:225902/100‑1 (MQ=255)
               gAGGTAAAATTATGCAGTCGGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACAGATTGCTGATGCGATCCATCAGCGGGc                                                                                      <  2:425246/100‑1 (MQ=255)
                aGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCGTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaa                                                                                        <  2:1550105/97‑3 (MQ=255)
                aGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCa                                                                                     >  2:1745242/1‑100 (MQ=255)
                 ggTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCaa                                                                                    >  2:2235831/1‑100 (MQ=255)
                   tAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCACTg                                                                                  >  2:2249244/1‑100 (MQ=255)
                   tAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATg                                                                                  >  1:1372474/1‑100 (MQ=255)
                    aaaaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATgg                                                                                 <  1:621829/100‑1 (MQ=255)
                    aaaaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaatatgtgc                                                                                 <  1:1992697/100‑10 (MQ=38)
                     aaaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGa                                                                                >  2:321298/1‑100 (MQ=255)
                      aaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAg                                                                               <  1:462973/100‑1 (MQ=255)
                          aTGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGGCAAGATTCATTGACCGCACATATTGCAGATGCGTACCATCAGCGGGCAATGGAGCgg                                                                            >  2:1253826/1‑99 (MQ=255)
                             cAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGaaaa                                                                        >  2:393314/1‑100 (MQ=255)
                              agccTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATGGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAAt                                                                       <  2:420434/97‑1 (MQ=255)
                              aGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAAt                                                                       >  1:2115935/1‑100 (MQ=255)
                               gTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATa                                                                      <  1:1040811/100‑1 (MQ=255)
                                tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                     >  2:2064102/1‑100 (MQ=255)
                                tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                     >  1:2128449/1‑100 (MQ=255)
                                tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                     >  1:1504570/1‑100 (MQ=255)
                                tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                     <  1:418829/100‑1 (MQ=255)
                                 cgatctgTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACa                                                                    <  2:1043518/94‑1 (MQ=255)
                                   gAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAgg                                                                  <  2:216545/100‑1 (MQ=255)
                                      cGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCCTCAGCGGGAAATGGCACGGAAAATaaccgt                                                                 >  1:1108431/1‑93 (MQ=255)
                                       gTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACaagatcg                                                              >  1:1043526/1‑94 (MQ=255)
                                         atttatttGGTATGGGCCCATCCTCGTCATGATTCATTTACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCTGAAAATACAGGTGAc                                                              >  2:2246480/1‑98 (MQ=255)
                                         atttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACgg                                                            >  1:1303892/1‑100 (MQ=255)
                                          tttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGa                                                           >  2:1888975/1‑100 (MQ=255)
                                          tttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGa                                                           >  1:1427814/1‑100 (MQ=255)
                                           ttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGaa                                                          <  1:431607/100‑1 (MQ=255)
                                            tatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAAc                                                         >  2:998002/1‑100 (MQ=255)
                                                tGGTATGGGCCCATCCTCGTCATGATTCATTGACCTCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGa                                                     <  1:1910944/100‑1 (MQ=255)
                                                 ggTATGGGCCCATCCTCGTCATGATTCATTTACCGCACATATTGCTGATACGATCCATCAGCGCGCAATGAAGCGGAAAATACAGGAGACAGAACTc                                                       >  2:713104/1‑97 (MQ=255)
                                                   tATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAttt                                                  <  1:1624623/100‑1 (MQ=255)
                                                   tATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAttt                                                  <  1:1598824/100‑1 (MQ=255)
                                                   gatctgCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAttt                                                  <  1:853004/95‑1 (MQ=255)
                                                    aTGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTta                                                 <  1:388638/100‑1 (MQ=255)
                                                       ggCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTatat                                              >  2:440791/1‑100 (MQ=255)
                                                        gCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTAGATc                                             >  2:852996/1‑100 (MQ=255)
                                                         cccATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCg                                            >  2:659624/1‑100 (MQ=255)
                                                           cATCCTCGTAATGATTCATTGACCGCACATATTGCTGATGAGATCCATCAGCGGGCAATGGAGCGGAAAATACATGTGACGGAACTCGAGTTATATCGGc                                          >  2:408301/1‑100 (MQ=255)
                                                            aTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                         >  2:840535/1‑100 (MQ=255)
                                                            aTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                         >  1:1978626/1‑100 (MQ=255)
                                                            aTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                         <  1:2151602/100‑1 (MQ=255)
                                                             tCCTCGTCATGATTCATTGACAGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                         <  2:2300964/99‑1 (MQ=255)
                                                              ccTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTa                                       >  1:583405/1‑100 (MQ=255)
                                                                   tCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTc                                  <  2:1086093/100‑1 (MQ=255)
                                                                    cATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCa                                 >  2:84536/1‑100 (MQ=255)
                                                                       gATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAAcc                              <  1:2537823/100‑1 (MQ=255)
                                                                           cATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGt                          >  1:802117/1‑100 (MQ=255)
                                                                           cATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGt                          <  1:1734648/100‑1 (MQ=255)
                                                                           cATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATAGCGGCGTAATTTCAACCCAGt                          <  2:547114/100‑1 (MQ=255)
                                                                            aTTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtg                         >  1:1147182/1‑100 (MQ=255)
                                                                            aTTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAGTTATATCGGCGTAATTTCAACCCAGtg                         >  2:569180/1‑100 (MQ=255)
                                                                             ttGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtga                        >  1:609004/1‑100 (MQ=255)
                                                                              tggccGCACATATTGCTGATGCGAGCCATCGGCGGGCAATGGAGCGGAAAATAAAGGTGACGGCACGCGATTTATAACGGCGCAATTTCAACCCCGtga                        >  2:2429941/4‑99 (MQ=255)
                                                                              tGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtgat                       <  1:808973/100‑1 (MQ=255)
                                                                               gACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtgatg                      >  1:1190506/1‑100 (MQ=255)
                                                                                aCCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGGACTCGATTTATATCGGCGTAATTTCAACCCAGtgatg                      <  2:1370490/99‑1 (MQ=255)
                                                                                  cGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGTCGTAATTTCAACCCAGTGATGACg                   <  2:929707/100‑1 (MQ=255)
                                                                                  cGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACg                   <  2:2459481/100‑1 (MQ=255)
                                                                                   gCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGc                  <  1:706346/100‑1 (MQ=255)
                                                                                   gCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGc                  >  1:292535/1‑100 (MQ=255)
                                                                                   gCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGc                  >  1:2268702/1‑100 (MQ=255)
                                                                                    cattgcccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGcc                 >  1:1633611/9‑100 (MQ=38)
                                                                                      caTATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCgg               <  2:1840429/100‑1 (MQ=255)
                                                                                        tatTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGGCGGCGGa              >  1:1665437/1‑99 (MQ=255)
                                                                                        gcccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGaa             >  1:2064094/5‑100 (MQ=255)
                                                                                        gcccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGaa             >  1:1223303/5‑100 (MQ=255)
                                                                                         cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATGGGCGTAATTTCAACCCAGTGATGACGCCGGAAg            >  2:294242/4‑100 (MQ=255)
                                                                                         cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAg            >  1:697636/4‑100 (MQ=255)
                                                                                         cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAg            >  1:1507335/4‑100 (MQ=255)
                                                                                         cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAg            >  1:1295061/4‑100 (MQ=255)
                                                                                          ccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGa           >  1:2027048/3‑100 (MQ=255)
                                                                                          ccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAATTATATCGGAGTAATTTCAACCCAGTGATGACGCAGGAAGa           >  2:2083797/3‑100 (MQ=255)
                                                                                             cTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGa        <  2:1020621/100‑1 (MQ=255)
                                                                                               gATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAc      <  2:673370/100‑1 (MQ=255)
                                                                                               gATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAc      >  1:959335/1‑100 (MQ=255)
                                                                                                aTGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAcc     <  1:2188456/100‑1 (MQ=255)
                                                                                                aTGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAcc     <  1:590471/100‑1 (MQ=255)
                                                                                                 tGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCa    <  2:528076/100‑1 (MQ=255)
                                                                                                   cGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGa  >  2:1045899/1‑100 (MQ=255)
                                                                                                   cGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGa  >  1:592560/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
CCAGAACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGA  >  NC_000913/949642‑949840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: