Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,073,307 Δ1 bp 100% coding (705/1149 nt) rutA ← pyrimidine oxygenase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,073,3050A.96.0% 427.9 / 9.7 101coding (707/1149 nt)rutApyrimidine oxygenase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (51/46);  total (52/49)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 103 total aligned reads displayed.

GTCGGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAG  >  NC_000913/1073208‑1073400
                                                                                                 |                                                                                               
gtcgGTTTCATCGGCAATCACCATAAGCAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaag                                                                                               <  2:716477/100‑2 (MQ=255)
gtcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaag                                                                                               >  2:177256/1‑99 (MQ=255)
     tttCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGGGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTg                                                                                         <  1:1344771/100‑1 (MQ=255)
       tCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTggg                                                                                       <  1:1833913/100‑1 (MQ=255)
          tCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtg                                                                                     <  2:1574060/99‑1 (MQ=255)
           cGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTa                                                                                   >  1:1143213/1‑100 (MQ=255)
                 tCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACg                                                                             <  2:1074220/100‑1 (MQ=255)
                 tCACCATAAACAACACATAAGAGCCAACGGCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACg                                                                             <  1:1151953/100‑1 (MQ=255)
                   aCCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGcc                                                                           >  2:2230970/1‑100 (MQ=255)
                    ccATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCTTCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCt                                                                          >  2:1643131/1‑100 (MQ=255)
                    ccATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCt                                                                          >  1:2391202/1‑100 (MQ=255)
                    ccATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCt                                                                          >  1:2110501/1‑100 (MQ=255)
                      aTAAACCACACATAAGAGCCAACGTCGCGTCCGGTTTGCGCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGTGCGAAGCCGCGGGTGTATTTACGCCttt                                                                        <  2:1184708/100‑1 (MQ=255)
                      aTAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCttt                                                                        >  1:487412/1‑100 (MQ=255)
                       tAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCttt                                                                        >  2:1383475/1‑99 (MQ=255)
                        aagcaacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTGTGCGGTCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTGCGCCTTTGc                                                                      <  2:2307202/97‑1 (MQ=255)
                        aaaCAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGc                                                                      <  2:2126603/100‑1 (MQ=255)
                         aacaacACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGc                                                                      <  2:2395147/100‑1 (MQ=255)
                         aacaacACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGcc                                                                     >  2:1268087/1‑100 (MQ=255)
                          acaacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCg                                                                    >  1:678143/1‑100 (MQ=255)
                           caacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGa                                                                   <  1:814990/100‑1 (MQ=255)
                             acacATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGaaa                                                                 >  1:912856/1‑100 (MQ=255)
                             acacATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGaaa                                                                 <  2:144729/100‑1 (MQ=255)
                              cacaTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAAc                                                                <  1:487824/100‑1 (MQ=255)
                                caTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACa                                                               >  2:2053111/1‑100 (MQ=255)
                                caTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACa                                                               >  1:1296821/1‑99 (MQ=255)
                                caTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAg                                                              >  1:375379/1‑100 (MQ=255)
                                   aaGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAg                                                           <  1:2076343/100‑1 (MQ=255)
                                   aaGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAg                                                           >  1:730875/1‑100 (MQ=255)
                                     gagCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGtt                                                         >  2:926527/1‑100 (MQ=255)
                                      agCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGttn                                                        <  2:1129336/100‑2 (MQ=255)
                                       gCCAACGTCGCGTCCGGTTTGCTCTGCTGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa                                                       <  2:1308202/100‑1 (MQ=255)
                                       gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa                                                       >  2:2454112/1‑100 (MQ=255)
                                       gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa                                                       <  2:2081669/100‑1 (MQ=255)
                                       gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa                                                       <  2:2110487/100‑1 (MQ=255)
                                       gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa                                                       >  1:1850053/1‑100 (MQ=255)
                                       gCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGa                                                       <  1:2083460/100‑1 (MQ=255)
                                             gTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCgg                                                 <  1:43065/100‑1 (MQ=255)
                                              tCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGc                                                <  2:2214829/100‑1 (MQ=255)
                                               cgcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCa                                               <  1:1421380/100‑1 (MQ=255)
                                                gcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCAt                                              <  2:585332/100‑1 (MQ=255)
                                                   tCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATAcc                                           <  1:2032540/100‑1 (MQ=255)
                                                      tgTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCggg                                        <  1:1077626/99‑1 (MQ=255)
                                                       gTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCggga                                       >  2:1077618/1‑99 (MQ=255)
                                                       gTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGc                                       >  2:1843486/1‑100 (MQ=255)
                                                       gTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGc                                       >  2:1335880/1‑100 (MQ=255)
                                                         ttGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCgg                                     >  1:1667571/1‑100 (MQ=255)
                                                          tGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGga                                    >  1:1575242/1‑100 (MQ=255)
                                                          tGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGga                                    <  2:606703/100‑1 (MQ=255)
                                                          tGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGga                                    >  1:969996/1‑100 (MQ=255)
                                                           gCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCgg                                     <  1:2053125/99‑1 (MQ=255)
                                                           gCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgag                                   >  2:936168/1‑100 (MQ=255)
                                                           gCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgag                                   >  1:86846/1‑100 (MQ=255)
                                                            ctctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgaga                                  >  2:1985559/1‑100 (MQ=255)
                                                            ctctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgaga                                  >  2:1670517/1‑100 (MQ=255)
                                                             tctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGaa                                 >  2:833021/1‑100 (MQ=255)
                                                             tctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGaa                                 >  1:288014/1‑100 (MQ=255)
                                                             tctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGaa                                 >  2:378554/1‑100 (MQ=255)
                                                               tGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACg                               <  1:24616/100‑1 (MQ=255)
                                                               tGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACg                               >  1:2245435/1‑100 (MQ=255)
                                                               tGCGGCCTGTGTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAAc                                <  2:895877/99‑1 (MQ=255)
                                                                gCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGc                              <  1:1715676/100‑1 (MQ=255)
                                                                gCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGc                              >  2:1891643/1‑100 (MQ=255)
                                                                 tctccTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGcc                             <  1:1468482/97‑1 (MQ=255)
                                                                  ggCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCa                            >  2:1921403/1‑100 (MQ=255)
                                                                   gCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCAt                           <  1:282848/100‑1 (MQ=255)
                                                                   gCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCAt                           <  1:701133/100‑1 (MQ=255)
                                                                    ccTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCaga                          >  2:1468471/1‑98 (MQ=255)
                                                                     cTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGGGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCAt                           >  2:61102/1‑98 (MQ=255)
                                                                     cTGTTTCATCCGCGCAGCGGTCGGGACGAAGCCGGGGATGTATTTACGCCGTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCAt                           >  2:1551073/1‑98 (MQ=255)
                                                                       gTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCa                       >  2:2320052/1‑100 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  2:2439180/100‑1 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    >  1:2085385/1‑100 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  1:1129886/100‑1 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  1:2420645/100‑1 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  1:799919/100‑1 (MQ=255)
                                                                           cATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGt                   >  1:2487764/1‑100 (MQ=255)
                                                                           cATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGt                   >  1:1755166/1‑100 (MQ=255)
                                                                           cATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGt                   >  1:610002/1‑100 (MQ=255)
                                                                              ccGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGc                <  1:115391/100‑1 (MQ=255)
                                                                              ccGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGc                <  2:678136/100‑1 (MQ=255)
                                                                              ccGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGc                <  2:1358612/100‑1 (MQ=255)
                                                                               cgcgCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCt               >  1:679998/1‑100 (MQ=255)
                                                                                gcggAGCGGTGGGGGCGACGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACTGGGCGGAGAACGCCATGCCa                       <  2:1569267/91‑1 (MQ=255)
                                                                                gcgcAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCtt              >  1:2097335/1‑100 (MQ=255)
                                                                                  gcAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACACCATGCCAGCGTCGCTTTg            <  1:530582/100‑1 (MQ=255)
                                                                                   cAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGc           <  1:2497059/100‑1 (MQ=255)
                                                                                   cAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGc           >  2:2360810/1‑100 (MQ=255)
                                                                                   cAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGc           >  1:1748637/1‑100 (MQ=255)
                                                                                    aGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGc           <  2:2097270/100‑1 (MQ=255)
                                                                                       gggcGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCgg       >  2:855335/4‑100 (MQ=255)
                                                                                       ggTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCgg       >  2:2539790/1‑100 (MQ=255)
                                                                                       ggTCGGGGCGAAGCCATGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCgg       <  1:2525993/100‑1 (MQ=255)
                                                                                          cGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGgcgc    <  2:745924/100‑1 (MQ=255)
                                                                                          cGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGgcgc    >  1:686713/1‑100 (MQ=255)
                                                                                           ggggCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCa   <  2:2475162/100‑1 (MQ=255)
                                                                                            gggCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCan  <  1:395382/100‑2 (MQ=255)
                                                                                            gggCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAg  >  1:1086753/1‑100 (MQ=255)
                                                                                            gggCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAg  >  2:763083/1‑100 (MQ=255)
                                                                                            gggCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAg  <  1:2377357/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GTCGGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAG  >  NC_000913/1073208‑1073400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: