Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,524,458:1 +T 15.7% intergenic (+90/+23) yncE → / ← ansP ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,524,4581.T15.7% 359.3 / 74.0 115intergenic (+90/+23)yncE/ansPATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base . (51/46);  new base T (10/8);  total (61/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

Only 100 of 122 total aligned reads displayed.

TGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAA  >  NC_000913/1524359‑1524557
                                                                                                    |                                                                                                   
tGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGc                                                                                                      <  1:226067/100‑1 (MQ=255)
tGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGc                                                                                                      <  1:226259/100‑1 (MQ=255)
 gcgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCa                                                                                                    >  2:2353729/1‑100 (MQ=255)
 gcgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCa                                                                                                    >  1:1499978/1‑100 (MQ=255)
 gcgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCa                                                                                                    <  1:418848/100‑1 (MQ=255)
    cTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGt                                                                                                 >  1:2471909/1‑100 (MQ=255)
     tGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTa                                                                                                >  1:120292/1‑100 (MQ=255)
      gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAg                                                                                               >  1:1136162/1‑100 (MQ=255)
       tAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGc                                                                                              >  2:1266201/1‑100 (MQ=255)
          tAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCgg                                                                                           <  2:2005861/100‑1 (MQ=255)
           aGATATGGCGCGGGAGACAACGGTGCGACTTGCATGAATTTTATCACGGCGTCTCAGGAACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                          <  2:1492635/100‑1 (MQ=255)
           aGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCggg                                                                                          <  2:2224960/100‑1 (MQ=255)
            gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGc                                                                                         <  1:2339412/100‑1 (MQ=255)
            gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGc                                                                                         <  1:1808140/100‑1 (MQ=255)
            gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAAATTGCCCGGCTACGTGCCGGGc                                                                                         <  2:2315573/100‑1 (MQ=255)
             atatGGCGTTCGTGAAAACGGTCAGACTTTCTTGCCTTTTATTACGGCGTATCCGGCACTTTCGGCAGTAATCTGAATTTGCCCTGCTACGTGCCGGGCa                                                                                        <  2:319752/100‑1 (MQ=255)
             atatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCa                                                                                        <  1:673296/100‑1 (MQ=255)
              tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaa                                                                                       <  1:592608/100‑1 (MQ=255)
                 ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAAttt                                                                                    >  1:2212527/1‑100 (MQ=255)
                  gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACAGCGTATCCGGCACTTTCGTCAGTAATCTTAAATTGCCCGGCTACGTGCCGGGCAAattc                                                                                   <  2:902309/100‑2 (MQ=37)
                   cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                  <  2:1843259/100‑1 (MQ=255)
                   cGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTg                                                                                  >  2:2375150/1‑100 (MQ=255)
                    gAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCaaattcag                                                                                 <  2:1945029/100‑7 (MQ=38)
                    gAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGc                                                                                 <  1:1432321/100‑1 (MQ=255)
                     aGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCa                                                                                <  1:660173/100‑1 (MQ=255)
                     aGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGAGCAATTTTGCa                                                                                <  1:1439871/100‑1 (MQ=255)
                      gtgTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                               <  2:255879/98‑1 (MQ=255)
                      gggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                               <  2:876128/100‑1 (MQ=255)
                      gggTCAAAACGTGTACACTTGCCTGCCTTTTATCACGGGGTATCCGGCACTGTCGTCAGTAATCTTAATTTGCCCGGCACGTAGCCGGGCAATTTTGCaa                                                                               <  2:1203978/100‑1 (MQ=255)
                       ggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAt                                                                              >  2:804988/1‑100 (MQ=255)
                       ggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAAt                                                                              >  2:688075/1‑100 (MQ=255)
                          gAAAACGGTGGTACTTGCCTGCGTTTTATCACGGCGTCTCCGGCACTTGCGTCAGTAATCTGAATTTGCCCGGCTACGTGCCGGGCAAattc                                                                                   <  2:2022453/92‑2 (MQ=25)
                           aaaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAAc                                                                          <  1:821476/100‑1 (MQ=255)
                               cGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGc                                                                      <  2:79294/100‑1 (MQ=255)
                               cGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGc                                                                      >  2:840984/1‑100 (MQ=255)
                                 gTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCg                                                                    <  1:827734/100‑1 (MQ=255)
                                  tGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGt                                                                   <  2:1422289/100‑1 (MQ=255)
                                   gagaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGtt                                                                  <  1:2090516/100‑1 (MQ=255)
                                         tgcctgccTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                            >  2:813394/1‑100 (MQ=255)
                                         tgcctgccTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                            <  1:345385/100‑1 (MQ=255)
                                         tgcctgccTTTTATCACGGCGTATCCGCCAATTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGc                                                            <  2:756156/100‑1 (MQ=255)
                                          gcctgcctTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCtt                                                          <  1:154913/100‑1 (MQ=255)
                                           cctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCtt                                                          >  2:1310397/1‑100 (MQ=255)
                                            ctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTa                                                         <  2:1775233/100‑1 (MQ=255)
                                               cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAac                                                      <  1:138162/100‑1 (MQ=255)
                                                ctTTTATCACGGAGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTGAAaca                                                     >  2:2509321/1‑100 (MQ=255)
                                                   ttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCCATTTTGCACTTAACTCGCCGTTTCAGGCTTAAAcaca                                                   >  2:1092245/1‑99 (MQ=255)
                                                     aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTATCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTCAACACAAtt                                                >  2:276205/1‑100 (MQ=255)
                                                     aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                <  1:698803/100‑1 (MQ=255)
                                                     aTCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt                                                >  1:1484585/1‑100 (MQ=255)
                                                        aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                             >  2:1169828/1‑100 (MQ=255)
                                                        aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                             >  1:1258511/1‑100 (MQ=255)
                                                        aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc                                             <  1:765755/100‑1 (MQ=255)
                                                         cGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCt                                            <  1:1078831/100‑1 (MQ=255)
                                                            cGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtt                                         >  1:494393/1‑100 (MQ=255)
                                                             gTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttt                                        <  1:1159407/100‑1 (MQ=255)
                                                              tATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt                                       <  2:134636/100‑1 (MQ=255)
                                                               aTCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttttt                                      >  1:2471036/1‑100 (MQ=255)
                                                                 ccGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                    <  2:199553/100‑1 (MQ=255)
                                                                 ccGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct                                    <  2:684259/100‑1 (MQ=255)
                                                                  cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt                                   <  2:1247645/100‑1 (MQ=255)
                                                                   ggCACTTTCGTGAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                  <  2:1716822/100‑1 (MQ=255)
                                                                   ggCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                  >  2:2471787/1‑100 (MQ=255)
                                                                   ggCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc                                  <  1:2069417/100‑1 (MQ=255)
                                                                       cTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCt                              >  2:662607/1‑100 (MQ=255)
                                                                        tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAGCTAGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTGTTGATc                               <  2:2020712/98‑1 (MQ=255)
                                                                        tttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCa                                 <  2:1030237/96‑1 (MQ=255)
                                                                         ttCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTc                            >  2:1593383/1‑100 (MQ=255)
                                                                           cGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt                          <  2:1359964/100‑1 (MQ=255)
                                                                             tCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                        <  1:179995/100‑1 (MQ=255)
                                                                             tCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg                        >  2:2376562/1‑100 (MQ=255)
                                                                              cAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcga                       >  1:846292/1‑100 (MQ=255)
                                                                              cAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcga                       >  2:2488368/1‑100 (MQ=255)
                                                                               aGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgac                      >  2:761529/1‑100 (MQ=255)
                                                                                gTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcgacg                     >  1:2235793/1‑100 (MQ=255)
                                                                                   aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                  >  1:1875676/1‑100 (MQ=255)
                                                                                   aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                  >  1:1875618/1‑100 (MQ=255)
                                                                                   aTCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                  >  1:402590/1‑100 (MQ=255)
                                                                                    tCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCGTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACt                  >  2:2018534/1‑99 (MQ=255)
                                                                                     ttGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg                <  1:2099947/99‑1 (MQ=255)
                                                                                     cTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg                >  1:838024/1‑100 (MQ=255)
                                                                                      tGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgga               >  2:2099933/1‑99 (MQ=255)
                                                                                      caaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgc               >  1:2435664/6‑100 (MQ=38)
                                                                                      caaaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCGTCCTCGACGACTGgc               >  1:2522403/6‑100 (MQ=38)
                                                                                      caaaattGCCCGGCTACGTGCCGGGAAATTTTGCAATTAACTCGCCGTTTCAGGCATAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                 >  2:1086463/6‑98 (MQ=37)
                                                                                        aaattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCGTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTg                 >  2:2077825/4‑96 (MQ=38)
                                                                                        aaTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcgc             >  1:370650/1‑100 (MQ=255)
                                                                                        aaTTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGACGTCTCCGGCTTAAACACAATTTCCTGTTTTCCTTCATCTTCCACgacgac                   >  2:840438/1‑94 (MQ=255)
                                                                                         aattGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCa            >  2:646877/3‑100 (MQ=255)
                                                                                          attGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTTCTGTTTTTCTTCTTCTTCCTCGACGCCTGgcgc             >  2:1642812/2‑98 (MQ=255)
                                                                                          attGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAg           >  1:1244878/2‑100 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTg         >  1:1007335/1‑100 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTAGACGACTGGCGCAGTg         >  2:2286468/1‑100 (MQ=255)
                                                                                            tGCCCGGCTACGTGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGCTTTTCTTCATCTTCCTCGACGACTGGCGCAGt          >  2:1224485/1‑99 (MQ=255)
                                                                                             gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCGAGCTTAAACACAATTTCCTGCTTTTCTTCATCTTCCTCGACGACTAGCGCAGttc        >  2:1004582/1‑98 (MQ=255)
                                                                                             gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGCCTGGCGCAGTGc        >  1:2275514/1‑100 (MQ=255)
                                                                                             gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGc        >  1:213344/1‑100 (MQ=255)
                                                                                             gCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGc        <  2:1950750/100‑1 (MQ=255)
                                                                                              cccGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt       >  1:1532042/1‑100 (MQ=255)
                                                                                                   cACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGaa  >  2:2104258/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
TGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAA  >  NC_000913/1524359‑1524557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: