Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,850,719:1 +T 24.7% intergenic (+26/‑141) sppA → / → ansA protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,7191.T24.7% 183.5 / 91.3 85intergenic (+26/‑141)sppA/ansAprotease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base . (38/26);  new base T (8/13);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

GTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCT  >  NC_000913/1850620‑1850815
                                                                                                    |                                                                                                
gtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc                                                                                                   <  1:1995199/100‑1 (MQ=255)
 tgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcg                                                                                                 >  1:2458933/1‑100 (MQ=255)
 tgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcg                                                                                                 >  1:2459025/1‑100 (MQ=255)
  gaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcgt                                                                                                <  1:2440773/100‑1 (MQ=255)
    cAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcgtcg                                                                                              >  2:820630/1‑100 (MQ=255)
    cAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtcgtcg                                                                                              >  2:1971970/1‑100 (MQ=255)
     aaGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCgg                                                                                             >  1:2451112/1‑100 (MQ=255)
       gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGcc                                                                                           <  1:339233/100‑1 (MQ=255)
       gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                           <  1:586719/100‑1 (MQ=255)
       gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                           <  1:1639395/100‑1 (MQ=255)
       gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                           <  2:1872245/100‑1 (MQ=255)
       gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                           <  1:1223040/100‑1 (MQ=255)
        cTGGTCGCGTTTAATGTCCCACAAAACCGTTATGCGTTTTGCCTTACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcg                                                                                           <  2:1306191/99‑2 (MQ=255)
         tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcan                                                                                         <  1:1707610/100‑2 (MQ=255)
         tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                         <  2:1113166/100‑1 (MQ=255)
         tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                         <  1:1653556/100‑1 (MQ=255)
         tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcac                                                                                         <  1:234064/100‑1 (MQ=255)
          ggCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCcaca                                                                                        >  2:1295272/1‑100 (MQ=255)
           gCCGCGTTTAATGGCCCACAATACCGTTATGCGTTTTGCCTGACCTGCGCCCACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGcc                                                                                           <  2:2538433/96‑1 (MQ=255)
           gCCGCGTTTAATGCCCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactc                                                                                       <  1:2410689/100‑3 (MQ=255)
           gCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCcaca                                                                                        >  1:1243066/1‑99 (MQ=255)
             cgcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACatta                                                                                     <  1:1679359/100‑1 (MQ=255)
             cgcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactca                                                                                      <  2:1772636/99‑4 (MQ=38)
              gcgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACattat                                                                                    <  2:1335606/100‑1 (MQ=255)
               cgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACGATCTGTCGGCcactcagta                                                                                   <  2:2179683/100‑7 (MQ=38)
                gTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAttt                                                                                  <  1:822258/100‑1 (MQ=255)
                   tAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAtttttt                                                                               <  1:129235/100‑1 (MQ=255)
                     aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTTAGTGGCCGACAGATCGTCGGCCACATTATTTTTTAc                                                                             <  1:1804010/100‑1 (MQ=255)
                     aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTAttttttgc                                                                             >  2:1557820/1‑98 (MQ=255)
                      tGACGCACAAAACCGTTATGCGTTTTGCCTGACGTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTAc                                                                             <  2:2024333/99‑1 (MQ=255)
                       gACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGt                                                                           >  1:1706076/1‑100 (MQ=255)
                        aCCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTc                                                                          >  1:682947/1‑100 (MQ=255)
                         cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                         <  2:71921/100‑1 (MQ=255)
                         cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcg                                                                         >  1:867021/1‑100 (MQ=255)
                         cccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGACACATTATTTTTTACGTc                                                                          >  2:2384573/1‑99 (MQ=255)
                          ccACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcga                                                                        >  1:165631/1‑100 (MQ=255)
                           cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                       >  1:1845613/1‑100 (MQ=255)
                           cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgac                                                                       >  2:3851/1‑100 (MQ=255)
                            acaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTcgacg                                                                      >  1:2532364/1‑100 (MQ=255)
                               aaaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAAt                                                                   >  1:2406261/1‑100 (MQ=255)
                                aaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATc                                                                  <  1:1339751/100‑1 (MQ=255)
                                   cGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctc                                                               >  1:155983/1‑100 (MQ=255)
                                    gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                              <  1:1160937/100‑1 (MQ=255)
                                    gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                              >  1:1363520/1‑100 (MQ=255)
                                    gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                              >  2:344056/1‑100 (MQ=255)
                                    gTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCctct                                                              >  2:31149/1‑100 (MQ=255)
                                      tATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTc                                                            >  2:366860/1‑100 (MQ=255)
                                        tGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTccc                                                          >  2:108197/1‑100 (MQ=255)
                                          cGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGc                                                        <  2:165629/100‑1 (MQ=255)
                                           gTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCt                                                       <  2:1626595/100‑1 (MQ=255)
                                               tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGttt                                                   >  2:300853/1‑100 (MQ=255)
                                                gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc                                                  >  2:527421/1‑100 (MQ=255)
                                                gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc                                                  <  1:1766297/100‑1 (MQ=255)
                                                 ccTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCg                                                 >  2:1949821/1‑100 (MQ=255)
                                                    gACCTGCGCCAACATGCGTTAAGTCATGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTGCGccc                                              <  2:1981401/100‑1 (MQ=255)
                                                     aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGcccc                                             <  1:783432/100‑1 (MQ=255)
                                                      ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCa                                            >  1:1627323/1‑100 (MQ=255)
                                                            ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc                                      >  1:2264552/1‑100 (MQ=255)
                                                             cAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTcc                                     >  1:1316714/1‑100 (MQ=255)
                                                             cAACATGCGTTAAATCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc                                      >  2:1586971/1‑99 (MQ=255)
                                                              aaCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCt                                    <  2:1379733/100‑1 (MQ=255)
                                                                cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCtt                                   >  1:1394980/1‑99 (MQ=255)
                                                                  tGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATc                                >  2:669306/1‑100 (MQ=255)
                                                                    cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCg                              <  1:1290581/100‑1 (MQ=255)
                                                                    cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTGATCCg                              <  2:1505154/100‑1 (MQ=255)
                                                                      ttAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCa                            <  2:672759/100‑1 (MQ=255)
                                                                         aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAg                         <  2:926844/100‑1 (MQ=255)
                                                                          gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGAGGAATCCTCTTCCCGCTGTTTCGACCCATAATTCATTCTcccactatt                        >  2:1810878/1‑93 (MQ=255)
                                                                          gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAg                         >  2:2328354/1‑99 (MQ=255)
                                                                          gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGt                        <  1:1156772/100‑1 (MQ=255)
                                                                            cTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTAt                      >  1:929039/1‑100 (MQ=255)
                                                                            cTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTAt                      <  1:590959/100‑1 (MQ=255)
                                                                                tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTGCTTATCCGCATAGTAt                      >  2:2134445/1‑96 (MQ=255)
                                                                                tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg                  >  1:2087884/1‑100 (MQ=255)
                                                                                tACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg                  >  1:1977331/1‑100 (MQ=255)
                                                                                  cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATccg                   >  1:369012/1‑95 (MQ=255)
                                                                                    atgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTCTCCGCATAGTATCAGGTgc               >  2:1899841/3‑99 (MQ=38)
                                                                                    atgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcg              >  1:2512726/3‑100 (MQ=255)
                                                                                     tgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATATTATCAGGTgcgc             >  1:1942693/2‑100 (MQ=255)
                                                                                     tgTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcgc             >  1:1431734/2‑100 (MQ=255)
                                                                                     tgTGGCCGACGATCTGTCGGCCACATGATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcgc             >  1:1563084/2‑100 (MQ=255)
                                                                                      gTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt            >  2:677860/1‑100 (MQ=255)
                                                                                      gTGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt            >  1:2480596/1‑100 (MQ=255)
                                                                                       tGGCCGACGATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTc           >  1:691190/1‑100 (MQ=255)
                                                                                        ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc          >  2:2496750/1‑100 (MQ=255)
                                                                                        ggCCGAAAGATCGTCTGCCACGTCATGACTTACGTCGACGAATCCTCTTCCCGCGGTTTCGCCCCATATTCCATTATCCGCATAGTATCAg                   <  2:646660/91‑1 (MQ=255)
                                                                                         gCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTccc         <  2:1953427/100‑1 (MQ=255)
                                                                                          ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc        >  1:1871179/1‑100 (MQ=255)
                                                                                          ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccc        <  2:899298/100‑1 (MQ=255)
                                                                                               aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGcc   <  2:2451098/100‑1 (MQ=255)
                                                                                                gATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCt  >  2:700489/1‑100 (MQ=255)
                                                                                                    |                                                                                                
GTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCT  >  NC_000913/1850620‑1850815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: