Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,865:1 +G 16.5% intergenic (+23/‑90) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8651.G16.5% 293.8 / 63.2 91intergenic (+23/‑90)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base . (36/40);  new base G (3/12);  total (39/52)
Fisher's exact test for biased strand distribution p-value = 8.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

GATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGG  >  NC_000913/1868767‑1868963
                                                                                                   |                                                                                                  
gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATAGTCTTAACAACCCTGGCACGGTACACATGtt                                                                                                   >  2:2357479/1‑100 (MQ=255)
 atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTa                                                                                                  >  1:1101654/1‑100 (MQ=255)
  tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACCTGTTAc                                                                                                 >  2:69283/1‑100 (MQ=255)
  tgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAc                                                                                                 >  2:950952/1‑100 (MQ=255)
    gAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                               >  1:2288563/1‑100 (MQ=255)
     aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                              <  2:1506116/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                             <  1:65923/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                             <  1:216220/100‑1 (MQ=255)
      aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                             <  1:2454387/100‑1 (MQ=255)
       aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                            <  1:547027/100‑1 (MQ=255)
        aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcc                                                                                           <  1:2277151/100‑1 (MQ=255)
        aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTAGTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                           <  2:2445982/100‑1 (MQ=255)
         aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                          <  1:2075886/100‑2 (MQ=39)
          ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                         <  1:2336955/100‑3 (MQ=39)
          ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                         <  1:1755181/100‑3 (MQ=39)
          ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                         <  1:1646636/100‑3 (MQ=39)
          ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccag                                                                                         <  1:1380951/100‑3 (MQ=39)
           gCTTCATCCGGAACCATGTGTGTTTACTTTGCGAATTGGTTGTGCGCGTACGTGAGTCGGCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagg                                                                                        <  2:2332712/100‑4 (MQ=12)
           gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTAc                                                                                        <  1:2538621/100‑1 (MQ=255)
           gCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagg                                                                                        <  2:922894/100‑4 (MQ=38)
            cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccaggg                                                                                       <  1:1263343/100‑5 (MQ=38)
             tACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATAGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                       <  2:165603/99‑1 (MQ=255)
              acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAAc                                                                                     >  1:791870/1‑100 (MQ=255)
               cacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGGTAACGGGCCTACAAc                                                                                     >  2:450552/1‑99 (MQ=255)
                 cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                                  <  2:791863/100‑1 (MQ=255)
                   cGTAAACGGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACACTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                                <  2:1575041/100‑1 (MQ=255)
                   cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAg                                                                                <  2:791025/100‑1 (MQ=255)
                    gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                               <  1:1389853/100‑1 (MQ=255)
                      aaaCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGa                                                                             <  2:1422601/100‑1 (MQ=255)
                        aCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAc                                                                           <  1:385844/100‑1 (MQ=255)
                           ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGt                                                                        >  2:380792/1‑100 (MQ=255)
                            gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTg                                                                       <  2:204809/100‑1 (MQ=255)
                             tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTgg                                                                      <  1:1407712/100‑1 (MQ=255)
                              gCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAGATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTggg                                                                     >  2:593876/1‑100 (MQ=255)
                              gCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTggg                                                                     <  1:1489817/100‑1 (MQ=255)
                               cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                                    <  1:2275761/100‑1 (MQ=255)
                               cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                                    >  1:1508767/1‑100 (MQ=255)
                                gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGcc                                                                   <  1:2460249/100‑1 (MQ=255)
                                   tACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTga                                                                >  1:463675/1‑100 (MQ=255)
                                     cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                              <  1:1939141/100‑1 (MQ=255)
                                     cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                              >  1:2553584/1‑100 (MQ=255)
                                     cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTgaga                                                              >  2:1961435/1‑100 (MQ=255)
                                       gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                            >  1:1582147/1‑100 (MQ=255)
                                        tgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGc                                                           <  2:2553568/100‑1 (MQ=255)
                                          cGAATGGTATTTGCGCGTAAGTAAATAGACTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg                                                         >  2:748103/1‑100 (MQ=255)
                                            aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                       >  1:720259/1‑100 (MQ=255)
                                            aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                       >  1:1976449/1‑100 (MQ=255)
                                             aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa                                                      >  1:118950/1‑100 (MQ=255)
                                                 tATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAAcaca                                                  >  2:1697694/1‑100 (MQ=255)
                                                  aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                                 >  1:1763927/1‑100 (MQ=255)
                                                     tGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                              <  2:2032822/100‑1 (MQ=255)
                                                        gcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAg                                           <  1:2187447/100‑1 (MQ=255)
                                                         cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                          >  2:1549327/1‑100 (MQ=255)
                                                          gtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAg                                           >  2:1611687/1‑98 (MQ=255)
                                                           tacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                        <  1:1910036/100‑1 (MQ=255)
                                                            acgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATa                                       <  2:2448415/100‑1 (MQ=255)
                                                             cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                                      >  1:1155878/1‑100 (MQ=255)
                                                             cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGACTACAAAGACAGCGAACCGGGGGCCTGAGAAGCGGCAACACAGGAGTAGCATAc                                      >  2:610962/1‑100 (MQ=255)
                                                              gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                                     <  1:1447061/100‑1 (MQ=255)
                                                               taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                                    <  1:881152/100‑1 (MQ=255)
                                                               taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg                                    <  1:1654570/100‑1 (MQ=255)
                                                                aaaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGt                                   >  2:1815728/1‑100 (MQ=255)
                                                                 aaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGtt                                  >  2:75211/1‑100 (MQ=255)
                                                                  aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                                 <  1:2237953/100‑1 (MQ=255)
                                                                  aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCCACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                                 <  2:1458609/100‑1 (MQ=255)
                                                                     gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                              <  1:1392739/100‑1 (MQ=255)
                                                                       cTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa                            >  2:1909642/1‑100 (MQ=255)
                                                                         tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATg                          <  1:427584/100‑1 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACCACGACAGCGAACCGTGGGCCTGCGAAGCGACACGCCAGGCGCAGCATCCAGCTgcgaaccg                          >  1:2297140/1‑92 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                         <  2:64337/100‑1 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                         <  2:336696/100‑1 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                         >  1:743576/1‑100 (MQ=255)
                                                                          aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                         <  2:227848/100‑1 (MQ=255)
                                                                            caacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                       <  1:2099201/100‑1 (MQ=255)
                                                                                 cTGGCCCGGTACACGTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg                  >  1:1165097/1‑100 (MQ=255)
                                                                                  aggCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                 >  1:1003066/2‑100 (MQ=39)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACACCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCGTACAGTTGGCAAATGTAGTACggggg               >  2:928155/1‑100 (MQ=255)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg               <  1:380796/100‑1 (MQ=255)
                                                                                    gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg                  >  1:302793/1‑97 (MQ=255)
                                                                                    gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg               >  1:1276967/1‑100 (MQ=255)
                                                                                     cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg              <  1:1852586/100‑1 (MQ=255)
                                                                                     cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg              >  1:1214506/1‑100 (MQ=255)
                                                                                      ccGGTACACATGTTACCGGGCCTACAGCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc             >  2:2312300/1‑100 (MQ=255)
                                                                                      ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc             <  2:2258612/100‑1 (MQ=255)
                                                                                      ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc             <  1:1038898/100‑1 (MQ=255)
                                                                                       cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa            >  1:1194116/1‑100 (MQ=255)
                                                                                          tACACATCCTACCGGGCCTACACCGACAGCGAACCGTGCGCCTGAGAAGCGGCCACACAGGCGTAGCATACAGTTGCCAAATGTAGTACGGGGGGCata          <  2:1491725/99‑1 (MQ=255)
                                                                                            cacaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAACATACAGTTGGCAAATGTAGTACGGGGGGCATATGa       >  1:1802152/1‑100 (MQ=255)
                                                                                             acaTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc      >  1:1675112/1‑100 (MQ=255)
                                                                                                tGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg   <  1:1361168/100‑1 (MQ=255)
                                                                                                 gTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTgg  >  1:246166/1‑100 (MQ=255)
                                                                                                 gTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTgg  <  2:2269258/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGG  >  NC_000913/1868767‑1868963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: