Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,607:1 +G 17.4% coding (443/822 nt) yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6071.G17.4% 356.6 / 81.6 109coding (443/822 nt)yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (50/40);  new base G (8/11);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-02

Only 100 of 111 total aligned reads displayed.

GTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAG  >  NC_000913/2449508‑2449705
                                                                                                    |                                                                                                  
gTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAc                                                                                                     <  1:2234273/100‑1 (MQ=255)
     cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCa                                                                                               >  1:1306468/1‑100 (MQ=255)
     cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCa                                                                                               >  1:2132259/1‑100 (MQ=255)
      tGCAGGGTGAGCAGTTTTGACTTACTGATATAATTCTTATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGTCTTTCa                                                                                               <  2:2429924/99‑1 (MQ=255)
      tGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAg                                                                                              >  1:557598/1‑100 (MQ=255)
        cAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGc                                                                                            <  1:1697510/100‑1 (MQ=255)
         aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcc                                                                                           <  1:67606/100‑1 (MQ=255)
             tGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTg                                                                                       >  1:2070919/1‑100 (MQ=255)
               aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGACCCCTGAt                                                                                     >  2:366911/1‑100 (MQ=255)
                  aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATag                                                                                   <  2:1239365/100‑2 (MQ=255)
                  aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                                                                                  <  2:1462340/100‑1 (MQ=255)
                    ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtc                                                                                 <  1:2198840/100‑4 (MQ=38)
                    ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                <  2:579604/100‑1 (MQ=255)
                    ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                <  1:1614300/100‑1 (MQ=255)
                     tttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtca                                                                                <  1:900328/100‑5 (MQ=38)
                       tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                             <  1:849901/100‑1 (MQ=255)
                         aCTTACTGACATAATTCTCATTCCATGAAACGTCATACGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGcc                                                                           >  1:270163/1‑100 (MQ=255)
                         aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgac                                                                           <  1:2320901/100‑3 (MQ=37)
                          cTTACTGACATAATTGTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgaca                                                                          <  1:1582688/100‑4 (MQ=25)
                          cTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgaca                                                                          <  2:381046/100‑4 (MQ=37)
                           ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                         <  1:1184045/100‑5 (MQ=25)
                           ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                         <  1:1511023/100‑5 (MQ=25)
                           ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                         <  2:444922/100‑5 (MQ=37)
                            tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGccccc                                                                        <  2:2522114/100‑1 (MQ=255)
                            tACTGACAAAATTCTCATTCCATGAAACGTCATAAGGTTGAAAATAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                         <  2:556537/99‑5 (MQ=21)
                             aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCt                                                                       >  1:2229503/1‑100 (MQ=255)
                              cTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg                                                                      <  1:935663/100‑1 (MQ=255)
                                gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTCACTATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                    <  2:2552084/100‑1 (MQ=255)
                                 aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacacgatttg                                                                   <  2:1940078/100‑11 (MQ=21)
                                  cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                  >  1:2114237/1‑100 (MQ=255)
                                  cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACCGACGGACAAATCGTGTCGTGAGTATCAGGCCCCTGATAAGTCACGCCCCCTGgag                                                                   >  2:1623240/1‑99 (MQ=255)
                                   aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                 >  1:771113/1‑100 (MQ=255)
                                   aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                 <  1:988917/100‑1 (MQ=255)
                                    tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAgg                                                                <  1:2338705/100‑1 (MQ=255)
                                       ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                             >  1:1132391/1‑100 (MQ=255)
                                       ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                             >  1:1165312/1‑100 (MQ=255)
                                         ctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                           <  2:1701709/100‑1 (MQ=255)
                                          tcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                          <  1:2310292/100‑1 (MQ=255)
                                            aTTCCATGAAACGTCATAAGGTTGAACAGAAACTTATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                        <  2:627969/100‑1 (MQ=255)
                                            aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                        >  2:225790/1‑100 (MQ=255)
                                            aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                        <  1:895163/100‑1 (MQ=255)
                                             ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                       >  1:1314239/1‑100 (MQ=255)
                                             ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATATGGCGCCTGATACGTCACGCCCCCTGAAGAGCCATCaga                                                         >  2:801806/1‑98 (MQ=255)
                                              tCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGCTACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                       >  2:703993/1‑99 (MQ=255)
                                               ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                     >  1:2250036/1‑100 (MQ=255)
                                               ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                     >  1:13988/1‑100 (MQ=255)
                                               ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                     >  1:1538678/1‑100 (MQ=255)
                                                cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                    >  2:1687271/1‑100 (MQ=255)
                                                cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                    >  1:1738622/1‑100 (MQ=255)
                                                 aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcatt                                                   <  1:249851/100‑1 (MQ=255)
                                                  tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattc                                                  <  2:610079/100‑1 (MQ=255)
                                                    aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                <  1:1228467/100‑1 (MQ=255)
                                                      aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                              <  1:225792/100‑1 (MQ=255)
                                                        gTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                            <  2:502123/100‑1 (MQ=255)
                                                         tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAGTATAAGGCCCCTGATCAGTAACGCCACCTGGAGAGGCTTCACAGTCATCGAtaataa                                           >  2:758236/1‑100 (MQ=255)
                                                           aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                         <  2:446188/100‑1 (MQ=255)
                                                           aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                         <  2:447405/100‑1 (MQ=255)
                                                               ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                     >  1:208585/1‑100 (MQ=255)
                                                               ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                     >  1:978355/1‑100 (MQ=255)
                                                                 ttGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                   <  2:2090136/100‑1 (MQ=255)
                                                                     aCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                               >  2:1303574/1‑100 (MQ=255)
                                                                        gAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                            >  2:1066272/1‑100 (MQ=255)
                                                                        gAAACGGATGGACAAATCGGGTCGTTACTATCAGGCCCCTGATACGGCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                            >  2:2040063/1‑100 (MQ=255)
                                                                             ggATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                       <  2:2092835/100‑1 (MQ=255)
                                                                              gATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGt                      <  2:2001881/100‑1 (MQ=255)
                                                                               aTGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                     <  1:580623/100‑1 (MQ=255)
                                                                                tGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTaa                    >  1:2077457/1‑100 (MQ=255)
                                                                                 ggACAAATCGTGTCGTGACTATCAGGCCCCTGATACGACACGCCCCCTGGAGAGGCTCCAGAGTCATTCAAAATAACCAACCATGGACAAGAAAAGTaa                    >  2:1115991/1‑99 (MQ=255)
                                                                                  gACAAATCGTGTCGTGATTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                  >  1:1572623/1‑100 (MQ=255)
                                                                                  gACAAATCGTGTCCTGACTATCAGGCCCCTGGTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAGGGACAAGAAAAGTAAGa                  >  1:1725512/1‑100 (MQ=255)
                                                                                   aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                 <  2:1098496/100‑1 (MQ=255)
                                                                                    cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                >  2:1898912/1‑100 (MQ=255)
                                                                                     aaaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGTCCAAGAAAAGTAAg                   >  1:443008/1‑96 (MQ=255)
                                                                                      aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa              >  1:2036819/1‑100 (MQ=255)
                                                                                      aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa              >  1:2036447/1‑100 (MQ=255)
                                                                                        tcgtgtcgtgACTATCAGGCCCCTGATGCGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAATCCAt            >  2:113805/1‑100 (MQ=255)
                                                                                         gggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc           >  1:630516/6‑100 (MQ=25)
                                                                                         gggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc           >  2:450929/6‑100 (MQ=25)
                                                                                           gggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtctc         >  2:366289/4‑100 (MQ=37)
                                                                                            gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGACAAGTAAGATTCAATctct        >  2:2209606/1‑100 (MQ=255)
                                                                                            gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGagccgaatca         >  2:360058/1‑90 (MQ=255)
                                                                                            gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  1:697953/1‑100 (MQ=255)
                                                                                            ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  1:1403256/3‑100 (MQ=37)
                                                                                             tcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg       <  1:2020752/100‑1 (MQ=255)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  1:429311/1‑100 (MQ=38)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  2:2202790/1‑100 (MQ=38)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  1:1413950/1‑100 (MQ=38)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  1:683753/1‑100 (MQ=38)
                                                                                              cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  2:1090042/1‑100 (MQ=255)
                                                                                              cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  2:867610/1‑100 (MQ=255)
                                                                                               gtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa     >  2:1235169/1‑100 (MQ=255)
                                                                                                tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag    <  1:486285/100‑1 (MQ=255)
                                                                                                tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag    <  1:2136581/100‑1 (MQ=255)
                                                                                                tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag    <  1:1097809/100‑1 (MQ=255)
                                                                                                tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag    <  1:310254/100‑1 (MQ=255)
                                                                                                tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa     >  2:464052/1‑99 (MQ=255)
                                                                                                 gACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga   >  1:1697477/1‑100 (MQ=255)
                                                                                                 gACTATCAGGCCCCTGATACCTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga   <  2:2467262/100‑1 (MQ=255)
                                                                                                  aCTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag  <  1:606492/100‑1 (MQ=255)
                                                                                                  aCTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag  <  2:1165304/100‑1 (MQ=255)
                                                                                                    |                                                                                                  
GTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAG  >  NC_000913/2449508‑2449705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: