Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,469,656:1 (T)7→8 100% coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T93.2% 232.3 / 9.7 74coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base T (28/41);  total (29/45)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469552‑2469743
                                                                                                  |                                                                                                 
tGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtt                                                                                                 >  1:685300/1‑100 (MQ=255)
 gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATTTAAAAACAATAAATCTATGCTTTTGCTAttt                                                                                                >  2:1161305/1‑100 (MQ=255)
 gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttt                                                                                                >  2:2511045/1‑100 (MQ=255)
 gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttt                                                                                                >  2:1645466/1‑100 (MQ=255)
 gTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttt                                                                                                >  2:1872128/1‑100 (MQ=255)
   ttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttt                                                                                             <  2:1814675/100‑1 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         >  1:1927607/1‑100 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         <  1:1667266/100‑1 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         <  1:1432805/100‑1 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         >  2:2411662/1‑100 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         <  2:906350/100‑1 (MQ=255)
     gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttt                                                                                           >  2:2411746/1‑99 (MQ=255)
      catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttt                                                                                        >  1:434243/1‑99 (MQ=255)
      catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttn                                                                                        <  2:423971/100‑2 (MQ=255)
        tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcg                                                                                      >  1:911270/1‑97 (MQ=255)
         catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgt                                                                                     >  1:1764876/1‑96 (MQ=255)
          atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgtt                                                                                    <  1:2355183/100‑6 (MQ=255)
          atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgtt                                                                                    <  1:1856882/100‑6 (MQ=255)
           ttCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                    <  1:720947/100‑1 (MQ=255)
            tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   >  1:571312/1‑100 (MQ=255)
              tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                                 <  1:889947/100‑1 (MQ=255)
               aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                <  1:1226396/100‑1 (MQ=255)
               aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttttttttttt                                                                                >  2:1468816/1‑90 (MQ=255)
                tCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaat                                                                               <  1:749798/100‑1 (MQ=255)
                 cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              <  2:31072/100‑1 (MQ=255)
                 cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaata                                                                              >  1:1465793/1‑100 (MQ=255)
                  aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTTGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                             <  2:878876/100‑1 (MQ=255)
                   agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            <  1:1485309/100‑1 (MQ=255)
                    gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                           <  1:396674/100‑1 (MQ=255)
                     agCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCTTTGCTaataatct                                                                          <  1:1740875/100‑3 (MQ=255)
                      gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATc                                                                         <  1:265098/100‑1 (MQ=255)
                      gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATGCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATc                                                                         <  1:99755/100‑1 (MQ=255)
                       cAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCt                                                                        <  1:1801008/100‑1 (MQ=255)
                          aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:993031/100‑1 (MQ=255)
                          aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:1481837/100‑1 (MQ=255)
                          aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:878646/100‑1 (MQ=255)
                            aGTTGTAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGc                                                                   <  1:1820946/100‑1 (MQ=255)
                            aGTTATAATTTTCCTTCCATTTTTGTTTTATTCATTAACTTGCAAAAACAATTAATCTATGCTTTTGCTATTTTTGTTCGTTGCTAATAATATCTACTGc                                                                   <  2:412196/100‑1 (MQ=255)
                                 taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              <  2:499144/100‑1 (MQ=255)
                                   aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTTTAATATCTACTCCTAAAAAt                                                            >  2:1914549/1‑100 (MQ=255)
                                   aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:988532/100‑1 (MQ=255)
                                    ttttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATc                                                           <  1:1262401/100‑1 (MQ=255)
                                       tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                        <  1:1872142/100‑1 (MQ=255)
                                       tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                        <  1:495939/100‑1 (MQ=255)
                                        ccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAtt                                                       <  1:2236038/100‑1 (MQ=255)
                                         cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                      >  1:296110/1‑100 (MQ=255)
                                           tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTTCTAATAATATCTACTGCTAAAAATCAATTtat                                                    >  2:1865490/1‑100 (MQ=255)
                                           tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtat                                                    >  2:1165358/1‑100 (MQ=255)
                                            ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  1:1625832/100‑1 (MQ=255)
                                              aTTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATAtt                                                 <  1:1120463/100‑1 (MQ=255)
                                                  ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  2:1089953/100‑1 (MQ=255)
                                                  ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             >  1:931235/1‑100 (MQ=255)
                                                     gtgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCa                                          >  1:2439755/1‑100 (MQ=255)
                                                     gtgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         <  1:1310227/100‑1 (MQ=255)
                                                      tgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         <  2:2044858/100‑1 (MQ=255)
                                                      tgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAc                                         <  2:2044925/100‑1 (MQ=255)
                                                       gtATTCATTAACATTCAAAAACAATAAATCTATGCTTTTGCTATTTTTTATCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  2:1259511/100‑1 (MQ=255)
                                                        tATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTa                                       <  1:1207701/100‑1 (MQ=255)
                                                         aTTCATTAACATGTAAAAACAATAAATCTCTGCTTTTGGTATTTTTTTTCGTTTCTTATAATATGTACTGTTATAAATCAATTTATATTAAcctacct                                        >  2:1579274/1‑93 (MQ=255)
                                                           tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    <  2:2544398/100‑1 (MQ=255)
                                                           tCATTAACATGCAAAAACAATAAACCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    >  1:2264546/1‑100 (MQ=255)
                                                            cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  2:2532927/1‑100 (MQ=255)
                                                            cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  1:1897331/1‑100 (MQ=255)
                                                            cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  1:88143/1‑100 (MQ=255)
                                                            cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   <  1:1198821/100‑1 (MQ=255)
                                                              ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                                 <  1:1539814/100‑1 (MQ=255)
                                                              ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                                 <  1:1427737/100‑1 (MQ=255)
                                                                  cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTTTAtt                             >  2:1456023/1‑100 (MQ=255)
                                                                  cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             >  2:1165191/1‑100 (MQ=255)
                                                                    tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           >  2:1286977/1‑100 (MQ=255)
                                                                    tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  1:2304262/1‑100 (MQ=255)
                                                                     gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  1:1123663/1‑100 (MQ=255)
                                                                     gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGATGCTAATAATATATACTGCTCAAAATCAATTTATATTAATCACACTGATAGTGTATTCat                          >  2:1985767/1‑100 (MQ=255)
                                                                      cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  1:124709/1‑100 (MQ=255)
                                                                      cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  1:210165/1‑99 (MQ=255)
                                                                      cAAAAACAATAAATATATTCGGTTGCGATTTTCTTTCGTTCCTAATAATATCTAATGCTAAAAACCAATTTATATTAACCCCACTAATGGTGTACTCata                         <  2:499856/100‑1 (MQ=255)
                                                                       aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        <  1:1991854/100‑1 (MQ=255)
                                                                       aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        >  1:1433104/1‑100 (MQ=255)
                                                                       aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        >  1:642342/1‑100 (MQ=255)
                                                                          aaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattat                     <  2:1122865/100‑1 (MQ=255)
                                                                            cAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatat                   >  1:2230252/1‑100 (MQ=255)
                                                                             aaTAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTACTTGTGTATTCatattatctt                  >  1:852710/1‑100 (MQ=255)
                                                                                 aaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTTACCCTACTAATAGTGTATTAATATTATAtttttt              >  2:1466924/1‑100 (MQ=255)
                                                                                  aTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt             <  2:1536674/100‑1 (MQ=255)
                                                                                   tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:1259189/100‑1 (MQ=255)
                                                                                   tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            >  1:1151454/1‑100 (MQ=255)
                                                                                    cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  1:2147632/100‑1 (MQ=255)
                                                                                     tATGCTTTTGCTATTTTTTTTTTTTTTTAATAATAAATACTGCTAAAAATCAATTTTTTTTTACCTCATTAATAGTTTATTCATTTTATAttttttttt           >  2:1482155/1‑98 (MQ=255)
                                                                                      aTGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt         <  2:1866967/100‑1 (MQ=255)
                                                                                       tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  2:718482/100‑1 (MQ=255)
                                                                                       tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  1:2511060/100‑1 (MQ=255)
                                                                                       tGCTTTTGCTATTTTTTTTCGTTGATAATAATATCTACTGTTAAAAATCAATTTATATTAACCTCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  2:443720/100‑1 (MQ=255)
                                                                                             tGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAcaca  >  1:344268/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469552‑2469743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: