Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,563,518:1 | (A)5→6 | 100% | intergenic (+401/‑74) | yffP → / → yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,563,513 | 1 | . | A | 98.5% | 228.4 / ‑3.4 | 67 | intergenic (+396/‑79) | yffP/yffQ | CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein |
Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (36/30); total (37/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 |
GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTA > NC_000913/2563415‑2563603 | ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a > 1:366553/1‑100 (MQ=255) ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a > 1:1927382/1‑100 (MQ=255) tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaa > 1:1718386/1‑100 (MQ=255) ngAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaa > 1:72072/2‑100 (MQ=255) aaTTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaa > 1:480374/1‑100 (MQ=255) tttGTGATGCAGATCGCGTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaaatg < 2:1507911/100‑4 (MQ=255) ttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑aaaaaatga > 2:742154/1‑96 (MQ=255) gtgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGaaa > 2:124147/1‑100 (MQ=255) tgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAAc < 1:2443156/100‑1 (MQ=255) gATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACg > 1:1688898/1‑100 (MQ=255) gATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACg > 1:2197857/1‑100 (MQ=255) gATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACg > 1:2226491/1‑100 (MQ=255) tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACTAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACgg > 2:1148199/1‑99 (MQ=255) tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGACAGGCAGTTCCTAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACgg > 2:2495488/1‑99 (MQ=255) tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGt > 2:30380/1‑100 (MQ=255) cAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTgg > 1:1484546/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa > 1:1625489/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa > 1:1989476/1‑100 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa < 2:521549/100‑1 (MQ=255) gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa > 1:340429/1‑100 (MQ=255) tCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAg > 1:943229/1‑100 (MQ=255) tttttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGc < 2:101653/100‑1 (MQ=255) ttCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCttt > 2:955109/1‑100 (MQ=255) tCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTa < 2:1939110/100‑1 (MQ=255) cccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTAc < 2:2070087/100‑1 (MQ=255) cccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTAc > 2:2285799/1‑100 (MQ=255) ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCACAACAACAAAATAAAGATAGAAAGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt < 2:683429/100‑1 (MQ=255) cTTCAGGCATTTTTGTTTAATTCAATGAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTg < 2:505899/100‑1 (MQ=255) cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg < 2:2418631/100‑1 (MQ=255) cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTg < 1:858460/100‑1 (MQ=255) cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGAAAAAAAAAAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGt < 2:693716/99‑1 (MQ=255) aGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGa > 1:1406912/1‑100 (MQ=255) aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt < 2:1059207/100‑1 (MQ=255) gTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt > 1:2154307/1‑100 (MQ=255) gTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt > 2:2191391/1‑100 (MQ=255) tttAATAAAATCAACCAAAGGCAGTTCCCATCAACGGAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTCGTGaaagcattg < 2:894339/97‑9 (MQ=255) ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAGTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg > 2:1368050/1‑100 (MQ=255) ttAACTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg < 1:2398906/100‑1 (MQ=255) tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg > 2:1512385/1‑99 (MQ=255) tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt > 1:1223526/1‑100 (MQ=255) tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt < 1:2125790/100‑1 (MQ=255) aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt > 1:1121595/1‑100 (MQ=255) ttCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTg > 2:2396462/1‑100 (MQ=255) aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGGGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg < 1:278801/100‑1 (MQ=255) cGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGa < 2:130624/100‑1 (MQ=255) aaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTNTGCTGTATTGAACt < 1:349128/100‑1 (MQ=255) gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg > 1:1497336/1‑100 (MQ=255) aGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGaa > 2:66578/1‑100 (MQ=255) aGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGGGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg < 2:545564/98‑1 (MQ=255) gTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAag < 2:1917960/100‑1 (MQ=255) gTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTGACTGGTGATGGTAATGTTGGTTTGCTGGATTGAACTGTGAag > 2:468040/1‑100 (MQ=255) cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag < 2:1650315/100‑1 (MQ=255) ccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg > 1:432529/1‑100 (MQ=255) caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg > 2:858504/1‑99 (MQ=255) caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga < 1:1805061/100‑1 (MQ=255) caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga > 1:1216016/1‑100 (MQ=255) caacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc < 2:943221/100‑1 (MQ=255) aacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGGTGGTAATGTTGGTTTGCTGTAGTGAAGTGTGAAGGAGGACGcc > 1:573310/1‑100 (MQ=255) aacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGcc > 1:962671/1‑100 (MQ=255) aacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGcc < 1:720793/100‑1 (MQ=255) acaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa > 2:2198514/1‑100 (MQ=255) acaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa > 1:603173/1‑100 (MQ=255) caaAATAAAGATGGGAGGT‑AAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATAGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATg > 2:2242179/1‑100 (MQ=255) aaaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATg < 2:2154293/100‑1 (MQ=255) aTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGaaa < 2:2168191/100‑1 (MQ=255) tAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc > 2:1820283/1‑100 (MQ=255) aaaGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAGACt > 2:136133/1‑100 (MQ=255) aaaGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACt < 1:66579/100‑1 (MQ=255) aGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt < 2:2514596/100‑1 (MQ=255) aGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt > 1:736822/1‑100 (MQ=255) gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt < 1:1178054/100‑1 (MQ=255) gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt > 1:372836/1‑100 (MQ=255) gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt < 1:2056406/100‑1 (MQ=255) aTGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTa < 1:2289241/100‑1 (MQ=255) | GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTA > NC_000913/2563415‑2563603 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |