Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,345:1 +T 18.6% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3451.T18.6% 340.1 / 88.1 113intergenic (+22/+468)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (54/38);  new base T (13/8);  total (67/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 118 total aligned reads displayed.

CCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGC  >  NC_000913/3183247‑3183444
                                                                                                   |                                                                                                   
   gCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAgcgc                                                                                                 >  1:303848/1‑100 (MQ=255)
   gCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAgcgc                                                                                                 >  1:1172898/1‑100 (MQ=255)
   gCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAgcgc                                                                                                 >  1:677743/1‑100 (MQ=255)
    cTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTACAGCGCa                                                                                                >  2:419963/1‑100 (MQ=255)
     tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                               >  1:443883/1‑100 (MQ=255)
       tGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                             <  1:2282497/100‑1 (MQ=255)
       tGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                             <  1:1644471/100‑1 (MQ=255)
         gCGTACTATGTGGGATGTCAGTGATGGGATTAAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                           >  1:1725567/1‑100 (MQ=255)
         gCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                           >  1:865684/1‑100 (MQ=255)
          cGTACTATGTGGAATGTCAGTGATGGGATTCATTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACa                                                                                          <  2:23660/100‑1 (MQ=255)
          cGTACTATGTGGAATGTCAGTGAGGGGATTCAGTTTAGTGCTGCTACAAACGGCGCGAAGTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACa                                                                                          <  2:732774/100‑1 (MQ=255)
              cTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACTACATCAGAAGTTGTCCATGCGCTGGACAACtt                                                                                      <  1:2429855/100‑1 (MQ=38)
              cTAGGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCGACAATCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGGCCATGCGCTGGACAACtt                                                                                      <  2:2020608/100‑1 (MQ=255)
                aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                     <  1:890047/99‑1 (MQ=38)
                aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACtt                                                                                      >  2:2204222/1‑98 (MQ=255)
                aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTc                                                                                     <  2:1995837/99‑1 (MQ=255)
                  gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                  >  2:69911/1‑100 (MQ=255)
                  gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                  <  1:1849572/100‑1 (MQ=255)
                  gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                  >  1:1030819/1‑100 (MQ=255)
                   tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAGCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgat                                                                                 <  2:2035783/100‑3 (MQ=37)
                   tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACAGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTGCTGcc                                                                                 <  2:304408/100‑1 (MQ=255)
                   tgGAATGTATGTGATGGGATTCAGTTAAGTCCTGCTACAAGTGGTGGGGATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACACCTTCTgat                                                                                 <  2:2056275/100‑3 (MQ=19)
                    ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                >  1:568231/1‑100 (MQ=255)
                    ggAACGTCAGTGATGGGATGCAGTGTAGTGCTGCTACAAAGGGAAGGAAAAAATTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatg                                                                                <  2:2493897/100‑4 (MQ=12)
                     gAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgt                                                                               <  2:2349316/100‑5 (MQ=37)
                          tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                          >  2:1268767/1‑100 (MQ=255)
                          tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                          >  2:904654/1‑100 (MQ=255)
                          tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                          >  2:2560501/1‑100 (MQ=255)
                           cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                         >  2:898426/1‑100 (MQ=255)
                           cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                         >  1:584752/1‑100 (MQ=255)
                           cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                         >  1:874230/1‑100 (MQ=255)
                            gatcttGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACg                                                                        <  2:181532/95‑1 (MQ=255)
                                 tGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGagatc                                                                   >  1:181534/1‑96 (MQ=255)
                                  gggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGcc                                                                  <  2:2414295/100‑1 (MQ=255)
                                    gATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                >  1:483647/1‑100 (MQ=255)
                                     aTTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTa                                                               >  2:507913/1‑100 (MQ=255)
                                      ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                              <  1:2301585/100‑1 (MQ=255)
                                      ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                              >  1:2245159/1‑100 (MQ=255)
                                      ttCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAt                                                              <  2:568224/100‑1 (MQ=255)
                                       tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                             <  1:2504376/100‑1 (MQ=255)
                                        ctgTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                            <  2:1406475/98‑1 (MQ=255)
                                        cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                            <  1:853161/100‑1 (MQ=255)
                                        cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                            >  1:1649589/1‑100 (MQ=255)
                                        cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                            <  2:865187/100‑1 (MQ=255)
                                          gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTcag                                                          >  1:1406486/1‑98 (MQ=255)
                                            ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        >  1:1443032/1‑100 (MQ=255)
                                            ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        <  2:1319377/100‑1 (MQ=255)
                                            ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        <  2:1597657/100‑1 (MQ=255)
                                             tAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCt                                                       <  1:1471303/100‑1 (MQ=255)
                                                 gctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTc                                                   >  2:2339014/1‑100 (MQ=255)
                                                  ctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                  <  1:2494048/100‑1 (MQ=255)
                                                    gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                <  2:2511769/100‑1 (MQ=255)
                                                    gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                >  1:1006727/1‑100 (MQ=255)
                                                     ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                               >  1:3188/1‑100 (MQ=255)
                                                     ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                               <  2:1820729/100‑1 (MQ=255)
                                                     ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                               >  2:314499/1‑100 (MQ=255)
                                                       aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                              >  1:703874/1‑99 (MQ=255)
                                                        cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtc                                            <  1:371237/100‑1 (MQ=255)
                                                          aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTTCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                           >  2:2263246/1‑99 (MQ=255)
                                                          aaCGGCGGCAATTGGTTAAAAACAACAGCCGAAGTTGTGCAGCTTATGGACAACTTCTGCCAGCTGCACGAAATCCTTATTCTACCTTTTCGGCGGtct                                           <  2:308768/99‑1 (MQ=255)
                                                             ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                       <  1:925550/100‑1 (MQ=255)
                                                             ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                       <  2:2509597/100‑1 (MQ=255)
                                                              gcggTAATTGGTTAAAAACAACATCAGAAGTTGTCCCGCGCATGGACAACTTCTGCCTGCTTCACGAAAGCCTTATTCTACCGTTTCGGCTGtctcttc                                       <  2:721780/99‑1 (MQ=255)
                                                              gcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                      >  2:1114882/1‑100 (MQ=255)
                                                               cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                     <  2:1011218/100‑1 (MQ=255)
                                                                  gAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                  <  1:194016/100‑1 (MQ=255)
                                                                   aaTTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGt                                 >  1:658155/1‑100 (MQ=255)
                                                                        gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                            >  1:461759/1‑100 (MQ=255)
                                                                         ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGTCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                           >  1:363425/1‑100 (MQ=255)
                                                                         ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                           >  1:1141868/1‑100 (MQ=255)
                                                                          tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          >  2:716791/1‑100 (MQ=255)
                                                                          tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          >  2:838790/1‑100 (MQ=255)
                                                                          tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          <  2:687321/100‑1 (MQ=255)
                                                                            aaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                        >  1:2329518/1‑100 (MQ=255)
                                                                                caacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                    >  2:392659/1‑100 (MQ=255)
                                                                                atctggcAGAAGTCGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAt                    >  1:1739915/7‑100 (MQ=37)
                                                                                   tggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                 >  2:39693/4‑100 (MQ=38)
                                                                                   caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                 >  2:1040978/1‑100 (MQ=255)
                                                                                    ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:1630488/3‑100 (MQ=38)
                                                                                    ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:958263/3‑100 (MQ=38)
                                                                                    aTCAGAAGTTGTCCAGCGCATTGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                <  2:1916719/100‑1 (MQ=255)
                                                                                    aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:1183755/1‑100 (MQ=255)
                                                                                    aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  2:886981/1‑100 (MQ=255)
                                                                                    aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:1515822/1‑100 (MQ=255)
                                                                                      cAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc              >  1:265561/1‑100 (MQ=38)
                                                                                       aGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc             >  1:1537305/1‑100 (MQ=38)
                                                                                        gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  2:471160/1‑100 (MQ=39)
                                                                                        gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  2:385615/1‑100 (MQ=39)
                                                                                        gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  1:2551069/1‑100 (MQ=39)
                                                                                        gAAGTTGTCCATGCGATGGACAACTTCTGCCAGCTGCACGAAAGCCTTATTCGACCTTTTCGGCTGTATATTCCCTGGTAATGGTTCAGTCTTTGCTct             >  2:44608/1‑98 (MQ=16)
                                                                                         aaGTTGTCCACCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc           <  2:1688131/100‑1 (MQ=255)
                                                                                          aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTctg            >  2:1097900/1‑96 (MQ=38)
                                                                                          aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg          >  1:100512/1‑100 (MQ=39)
                                                                                          aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc           >  2:2092644/1‑99 (MQ=39)
                                                                                           gctttCCAGCGCATGGACACCTTCTGGCAGCTTCCCTAAAGCTTTATGCTACCTTTTCGGCTGTCTCTGCTCTCGTCCGGTTTAAGTATTTGTTCcgc           <  2:492358/94‑1 (MQ=255)
                                                                                            ttGTCCAGCGCATGGACAACTTCTGCCAGCTTCTCGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg          <  2:1237266/98‑1 (MQ=255)
                                                                                              gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc      >  1:1980861/1‑100 (MQ=255)
                                                                                                 cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAg   <  2:283557/100‑1 (MQ=255)
                                                                                                 cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAg   >  1:2123226/1‑100 (MQ=255)
                                                                                                  aGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc  <  2:1887882/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGC  >  NC_000913/3183247‑3183444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: