| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 497,139:1 | (T)5→6 | 100% | intergenic (+145/‑36) | htpG → / → adk | protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 497,134 | 1 | . | T | 92.6% | 207.9 / 11.0 | 68 | intergenic (+140/‑41) | htpG/adk | protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base T (35/28); total (37/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑TTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCA > NC_000913/497035‑497227 | tttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGc < 1:1948541/100‑1 (MQ=255) tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑tt < 1:562208/100‑1 (MQ=255) tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑tt < 1:1669569/100‑1 (MQ=255) catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttt > 2:394717/1‑100 (MQ=255) catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttt > 2:1473199/1‑100 (MQ=255) tcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttttt > 1:334196/1‑100 (MQ=255) catcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑tttttt < 1:325656/100‑2 (MQ=255) tcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttttttca > 2:136715/1‑97 (MQ=255) tatGCACTTTCCGGAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttttttca > 1:1727286/3‑97 (MQ=255) cTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttttttcaa < 2:1532542/100‑5 (MQ=255) tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttttttcaaa < 1:876248/100‑6 (MQ=255) tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑ttttttcaaa > 1:924493/1‑95 (MQ=255) gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑TTTTT‑Tcaaaa < 1:2179471/100‑1 (MQ=255) tccgatctgCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑TTTTT‑Caaaaaa < 1:2539046/92‑1 (MQ=255) cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAAt > 2:506075/1‑100 (MQ=255) ttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTc < 1:1467846/100‑1 (MQ=255) cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑TAAAAAATTCGac > 2:467601/1‑100 (MQ=255) cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAAttt > 2:1735560/1‑96 (MQ=255) cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGac < 2:1880178/100‑1 (MQ=255) cGCAAATTATCTCGCCATTAACCGTTTCAGCCACAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGac > 1:2425901/1‑100 (MQ=255) gCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGcca > 1:1065313/1‑100 (MQ=255) gCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑TTTTT‑Caaaaaaagatcgga > 2:2539031/1‑92 (MQ=255) cAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGacac > 1:295743/1‑100 (MQ=255) aaaTTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGAcaca > 2:811899/1‑100 (MQ=255) aaTTATGTCGCCATTAACCGTTTCCGCCCCAGGTGCCTTTCTTTAGTCAATCGCCTGTTTGTGGTATCTTTTATCGCTTTTTT‑CAAAAAATTCGacac < 2:1976131/98‑1 (MQ=255) gCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAgggg > 1:1093921/1‑100 (MQ=255) cATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAggggct > 2:1549023/1‑98 (MQ=255) aTTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGAtt > 1:755162/1‑100 (MQ=255) tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CATTAAATTTGAAACAGATTACGGGGAtttt > 2:1752955/1‑100 (MQ=255) tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGAtttt < 1:1623955/100‑1 (MQ=255) tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGAtttt > 2:2439299/1‑100 (MQ=255) aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTc > 2:2424896/1‑99 (MQ=255) aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCg > 1:2244778/1‑100 (MQ=255) aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCg < 1:1983214/100‑1 (MQ=255) aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCg < 1:1452315/100‑1 (MQ=255) ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGc < 1:2177105/100‑1 (MQ=255) cgtgttaGCCTCAGGTGTCTTTTTTGAGGCAATCGCATGTTGCTGGGATCGTGTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGc < 2:2150622/93‑1 (MQ=255) cGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCa > 1:680841/1‑100 (MQ=255) cGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCa < 1:1190891/100‑1 (MQ=255) ttCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATg < 1:1283209/100‑1 (MQ=255) tCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGc > 1:2328736/1‑100 (MQ=255) cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg < 1:1715083/100‑1 (MQ=255) gCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTa > 1:82618/1‑100 (MQ=255) gCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTa > 1:1777984/1‑100 (MQ=255) cctggTGCGTTTCTTTAGGCAATCTCCTGTTGGTGGTATCTTTTATCGC‑TTTTTGCAAAAAATTCGACACATTTTATTGGGATTTTCGCAATGCGTAt < 2:1447048/95‑1 (MQ=255) ccTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCt > 2:700708/1‑100 (MQ=255) tttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTg < 1:229655/100‑1 (MQ=255) tttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTg < 2:583821/100‑1 (MQ=255) tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc < 1:2378642/100‑1 (MQ=255) cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg > 2:2463685/1‑100 (MQ=255) cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg < 1:639483/100‑1 (MQ=255) gAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑TTTTTTTTTTTACACATTTTAAGGGGATTTTTGCATTGCGTTTTATTCTGCTTGGCGCt > 2:1755601/1‑99 (MQ=255) aGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTcc < 2:316086/100‑1 (MQ=255) aaTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgc > 1:2245949/1‑100 (MQ=255) aaTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgc < 1:394721/100‑1 (MQ=255) aaTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgc > 2:2425284/1‑100 (MQ=255) aaTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATGTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgc > 2:444326/1‑100 (MQ=255) aTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAATGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg < 2:2064849/100‑1 (MQ=255) aTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg < 1:1830010/100‑1 (MQ=255) cGCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGcgcg < 1:1303601/100‑1 (MQ=255) gCCTGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg > 2:1468393/1‑100 (MQ=255) tGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg > 1:189111/1‑99 (MQ=255) tGTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGa < 1:757545/100‑1 (MQ=255) gTTGGTGGTATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGaa > 1:1838223/1‑100 (MQ=255) ggtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg < 2:2403244/100‑1 (MQ=255) ggtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg < 2:629487/100‑1 (MQ=255) ggtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg < 1:1159347/100‑1 (MQ=255) gtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg < 1:1941480/100‑1 (MQ=255) gtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg > 1:155203/1‑100 (MQ=255) gtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg > 1:1453228/1‑100 (MQ=255) gtggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATGTTAAGGGGATTTTCGCAATGCGTATCATTCTGGTTGGCGCTCCGGGCGCGGGGAAAggg < 1:1179743/100‑1 (MQ=255) gtggtATCGTTTATCGC‑TTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGa > 2:2127968/1‑100 (MQ=255) ggtATCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc > 2:2473382/1‑100 (MQ=255) aTCGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCa < 2:299953/100‑1 (MQ=255) cGTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAgg > 2:2077375/1‑100 (MQ=255) gTTTATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGTGCTCCGGGCGCGGGGAAAGGGACTCAGGc > 2:1666674/1‑100 (MQ=255) gTTTATCGCTTTTTT‑CAAAAAATTCGACACATATTAAGGGGATTTTTGCAATGCCTATCATTCTGCTCGGCGCTCCAGACGCGCGGGATGGGACTCa > 2:2065904/1‑97 (MQ=255) tATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa > 1:428195/1‑100 (MQ=255) tATCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa < 1:1216504/100‑1 (MQ=255) aTCGCTTTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGAGAGGGGAAAGGGACTCAGGCTCa > 2:2385947/1‑99 (MQ=255) | TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGC‑TTTTT‑CAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCA > NC_000913/497035‑497227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |