Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 949,745:1 +G 15.1% coding (78/591 nt) ycaK → putative NAD(P)H‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913949,7451.G15.1% 301.7 / 58.2 93coding (78/591 nt)ycaKputative NAD(P)H‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (43/36);  new base G (9/5);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

AACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGACTGG  >  NC_000913/949646‑949844
                                                                                                    |                                                                                                   
aaCTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATc                                                                                                      <  2:1865951/100‑1 (MQ=255)
aaCTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATc                                                                                                      <  1:2156766/100‑1 (MQ=255)
  cTTAACGTGGAGGTAAAATTATGCAGTCTGATCGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCa                                                                                                   <  2:1179275/100‑1 (MQ=255)
  cTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCa                                                                                                   <  2:1962356/100‑1 (MQ=255)
  cTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCa                                                                                                   >  2:1234556/1‑100 (MQ=255)
    tAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATc                                                                                                 <  1:1293156/100‑1 (MQ=255)
      aCGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAg                                                                                               >  1:56347/1‑100 (MQ=255)
       cGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGc                                                                                              >  1:403934/1‑100 (MQ=255)
        gTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGca                                                                                             <  2:495415/100‑2 (MQ=255)
         tGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaa                                                                                            <  1:2559255/100‑3 (MQ=255)
         tGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaa                                                                                            <  1:2335227/100‑3 (MQ=255)
           gAGGTAAAATTATGCAGTCTGAACGTATTTATGTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGc                                                                                          <  2:225902/100‑1 (MQ=255)
           gAGGTAAAATTATGCAGTCGGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACAGATTGCTGATGCGATCCATCAGCGGGc                                                                                          <  2:425246/100‑1 (MQ=255)
            aGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCGTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaa                                                                                            <  2:1550105/97‑3 (MQ=255)
            aGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCa                                                                                         >  2:1745242/1‑100 (MQ=255)
             ggTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCaa                                                                                        >  2:2235831/1‑100 (MQ=255)
               tAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCACTg                                                                                      >  2:2249244/1‑100 (MQ=255)
               tAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATg                                                                                      >  1:1372474/1‑100 (MQ=255)
                aaaaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATgg                                                                                     <  1:621829/100‑1 (MQ=255)
                aaaaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGGATCGCATCAGcaatatgtgc                                                                                     <  1:1992697/100‑10 (MQ=38)
                 aaaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGa                                                                                    >  2:321298/1‑100 (MQ=255)
                  aaTTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAg                                                                                   <  1:462973/100‑1 (MQ=255)
                      aTGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGGCAAGATTCATTGACCGCACATATTGCAGATGCGTACCATCAGCGGGCAATGGAGCgg                                                                                >  2:1253826/1‑99 (MQ=255)
                         cAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGaaaa                                                                            >  2:393314/1‑100 (MQ=255)
                          agccTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATGGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAAt                                                                           <  2:420434/97‑1 (MQ=255)
                          aGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAAt                                                                           >  1:2115935/1‑100 (MQ=255)
                           gTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATa                                                                          <  1:1040811/100‑1 (MQ=255)
                            tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                         <  1:418829/100‑1 (MQ=255)
                            tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                         >  2:2064102/1‑100 (MQ=255)
                            tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                         >  1:1504570/1‑100 (MQ=255)
                            tCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATAc                                                                         >  1:2128449/1‑100 (MQ=255)
                             cgatctgTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACa                                                                        <  2:1043518/94‑1 (MQ=255)
                               gAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAgg                                                                      <  2:216545/100‑1 (MQ=255)
                                  cGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCCTCAGCGGGAAATGGCACGGAAAATaaccgt                                                                     >  1:1108431/1‑93 (MQ=255)
                                   gTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACaagatcg                                                                  >  1:1043526/1‑94 (MQ=255)
                                     atttatttGGTATGGGCCCATCCTCGTCATGATTCATTTACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCTGAAAATACAGGTGAc                                                                  >  2:2246480/1‑98 (MQ=255)
                                     atttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACgg                                                                >  1:1303892/1‑100 (MQ=255)
                                      tttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGa                                                               >  1:1427814/1‑100 (MQ=255)
                                      tttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGa                                                               >  2:1888975/1‑100 (MQ=255)
                                       ttatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGaa                                                              <  1:431607/100‑1 (MQ=255)
                                        tatttGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAAc                                                             >  2:998002/1‑100 (MQ=255)
                                            tGGTATGGGCCCATCCTCGTCATGATTCATTGACCTCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGa                                                         <  1:1910944/100‑1 (MQ=255)
                                             ggTATGGGCCCATCCTCGTCATGATTCATTTACCGCACATATTGCTGATACGATCCATCAGCGCGCAATGAAGCGGAAAATACAGGAGACAGAACTc                                                           >  2:713104/1‑97 (MQ=255)
                                               tATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAttt                                                      <  1:1598824/100‑1 (MQ=255)
                                               tATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAttt                                                      <  1:1624623/100‑1 (MQ=255)
                                               gatctgCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAttt                                                      <  1:853004/95‑1 (MQ=255)
                                                aTGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTta                                                     <  1:388638/100‑1 (MQ=255)
                                                   ggCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTatat                                                  >  2:440791/1‑100 (MQ=255)
                                                    gCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTAGATc                                                 >  2:852996/1‑100 (MQ=255)
                                                     cccATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCg                                                >  2:659624/1‑100 (MQ=255)
                                                       cATCCTCGTAATGATTCATTGACCGCACATATTGCTGATGAGATCCATCAGCGGGCAATGGAGCGGAAAATACATGTGACGGAACTCGAGTTATATCGGc                                              >  2:408301/1‑100 (MQ=255)
                                                        aTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                             >  2:840535/1‑100 (MQ=255)
                                                        aTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                             <  1:2151602/100‑1 (MQ=255)
                                                        aTCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                             >  1:1978626/1‑100 (MQ=255)
                                                         tCCTCGTCATGATTCATTGACAGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCg                                             <  2:2300964/99‑1 (MQ=255)
                                                          ccTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTa                                           >  1:583405/1‑100 (MQ=255)
                                                               tCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTc                                      <  2:1086093/100‑1 (MQ=255)
                                                                cATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCa                                     >  2:84536/1‑100 (MQ=255)
                                                                   gATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAAcc                                  <  1:2537823/100‑1 (MQ=255)
                                                                       cATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGt                              >  1:802117/1‑100 (MQ=255)
                                                                       cATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGt                              <  1:1734648/100‑1 (MQ=255)
                                                                       cATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATAGCGGCGTAATTTCAACCCAGt                              <  2:547114/100‑1 (MQ=255)
                                                                        aTTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtg                             >  1:1147182/1‑100 (MQ=255)
                                                                        aTTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAGTTATATCGGCGTAATTTCAACCCAGtg                             >  2:569180/1‑100 (MQ=255)
                                                                         ttGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtga                            >  1:609004/1‑100 (MQ=255)
                                                                          tggccGCACATATTGCTGATGCGAGCCATCGGCGGGCAATGGAGCGGAAAATAAAGGTGACGGCACGCGATTTATAACGGCGCAATTTCAACCCCGtga                            >  2:2429941/4‑99 (MQ=255)
                                                                          tGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtgat                           <  1:808973/100‑1 (MQ=255)
                                                                           gACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGtgatg                          >  1:1190506/1‑100 (MQ=255)
                                                                            aCCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGGACTCGATTTATATCGGCGTAATTTCAACCCAGtgatg                          <  2:1370490/99‑1 (MQ=255)
                                                                              cGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGTCGTAATTTCAACCCAGTGATGACg                       <  2:929707/100‑1 (MQ=255)
                                                                              cGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACg                       <  2:2459481/100‑1 (MQ=255)
                                                                               gCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGc                      <  1:706346/100‑1 (MQ=255)
                                                                               gCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGc                      >  1:2268702/1‑100 (MQ=255)
                                                                               gCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGc                      >  1:292535/1‑100 (MQ=255)
                                                                                cattgcccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGcc                     >  1:1633611/9‑100 (MQ=38)
                                                                                  caTATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCgg                   <  2:1840429/100‑1 (MQ=255)
                                                                                    tatTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGGCGGCGGa                  >  1:1665437/1‑99 (MQ=255)
                                                                                    gcccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGaa                 >  1:1223303/5‑100 (MQ=255)
                                                                                    gcccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGaa                 >  1:2064094/5‑100 (MQ=255)
                                                                                     cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATGGGCGTAATTTCAACCCAGTGATGACGCCGGAAg                >  2:294242/4‑100 (MQ=255)
                                                                                     cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAg                >  1:1507335/4‑100 (MQ=255)
                                                                                     cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAg                >  1:1295061/4‑100 (MQ=255)
                                                                                     cccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAg                >  1:697636/4‑100 (MQ=255)
                                                                                      ccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGa               >  1:2027048/3‑100 (MQ=255)
                                                                                      ccgCTGATGGATCGCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGAATTATATCGGAGTAATTTCAACCCAGTGATGACGCAGGAAGa               >  2:2083797/3‑100 (MQ=255)
                                                                                         cTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGa            <  2:1020621/100‑1 (MQ=255)
                                                                                           gATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAc          <  2:673370/100‑1 (MQ=255)
                                                                                           gATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAc          >  1:959335/1‑100 (MQ=255)
                                                                                            aTGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAcc         <  1:2188456/100‑1 (MQ=255)
                                                                                            aTGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAAcc         <  1:590471/100‑1 (MQ=255)
                                                                                             tGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCa        <  2:528076/100‑1 (MQ=255)
                                                                                               cGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGa      >  2:1045899/1‑100 (MQ=255)
                                                                                               cGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGa      >  1:592560/1‑100 (MQ=255)
                                                                                                 aTCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGAcn    <  1:1300122/100‑2 (MQ=255)
                                                                                                  tCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCTGCGTAATTTCAACCCAGTGATGACACCGGAAAATGAACCATACTg   >  2:606073/1‑100 (MQ=255)
                                                                                                   ccATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGACTgg  <  2:549136/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
AACTTAACGTGGAGGTAAAATTATGCAGTCTGAACGTATTTATTTGGTATGGGCCCATCCTCGTCATGATTCATTGACCGCACATATTGCTGATGCGATCCATCAGCGGGCAATGGAGCGGAAAATACAGGTGACGGAACTCGATTTATATCGGCGTAATTTCAACCCAGTGATGACGCCGGAAGATGAACCAGACTGG  >  NC_000913/949646‑949844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: