Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,307,634	T→A	100%	I338F (ATC→TTC)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,634	0	T	A	100.0%	281.6 / ‑6.7	84	I338F (ATC→TTC)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (35/47);  minor base C (0/1);  total (35/49)

CGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGT  >  NC_000913/1307539‑1307729                                                                                                |                                                                                                cgGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACaaa                                                                                             >  1:2028825/1‑100 (MQ=255)  ggAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAat                                                                                            >  1:356401/1‑100 (MQ=255)  ggAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAat                                                                                            <  2:2189589/100‑1 (MQ=255)   gAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAata                                                                                           <  2:1931743/100‑1 (MQ=255)       gATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCa                                                                                       >  2:572516/1‑100 (MQ=255)          aataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcgc                                                                                    <  2:1466582/100‑1 (MQ=255)          aataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcgc                                                                                    <  2:130233/100‑1 (MQ=255)           ataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcgcg                                                                                   <  1:865379/100‑1 (MQ=255)              cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCg                                                                                >  2:455444/1‑100 (MQ=255)              cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCg                                                                                >  1:358421/1‑100 (MQ=255)                gACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGaa                                                                              <  2:137912/100‑1 (MQ=255)                gACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCATGGCACAAAGTAGGGCGTGGTCATGAACAAATACTCACGCGCCGaa                                                                              >  1:2547351/1‑100 (MQ=255)                 aCATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAAt                                                                             <  1:432251/100‑1 (MQ=255)                  cATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATTAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATa                                                                            <  1:848198/100‑1 (MQ=255)                  cATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTACATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATa                                                                            >  2:2363890/1‑100 (MQ=255)                     ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATaaac                                                                         >  1:1094442/1‑98 (MQ=255)                      cACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGcc                                                                        >  2:1667337/1‑100 (MQ=255)                      cACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGcc                                                                        <  2:278139/100‑1 (MQ=255)                       aCCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCg                                                                       >  1:1778640/1‑100 (MQ=255)                        ccccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGc                                                                      >  2:993116/1‑100 (MQ=255)                         cccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGc                                                                      <  1:240905/99‑1 (MQ=255)                            gcgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCCCGCGCCGAATAAGCCGCAGTc                                                                  <  1:2143932/100‑1 (MQ=255)                              gcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCaa                                                                <  1:1634649/100‑1 (MQ=255)                                      gccgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCt                                                        <  1:570257/100‑1 (MQ=255)                                        cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                      <  1:1692147/100‑1 (MQ=255)                                        cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                      >  1:2469820/1‑100 (MQ=255)                                        cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                      <  1:1152338/100‑1 (MQ=255)                                         gTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAACTAGGGCGTGGTCATGAACATATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTggn                                                     <  2:1396200/100‑2 (MQ=255)                                          tGCAAGTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGACCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                    <  2:476944/100‑1 (MQ=255)                                          tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                    <  2:208218/100‑1 (MQ=255)                                          tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                    <  1:1175527/100‑1 (MQ=255)                                           gCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGa                                                   <  1:2132327/100‑1 (MQ=255)                                           gCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGa                                                   >  1:1184616/1‑100 (MQ=255)                                            cAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGaa                                                  >  1:1932292/1‑100 (MQ=255)                                               aTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATga                                               <  2:631702/100‑1 (MQ=255)                                                tCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGCACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATga                                               <  1:1790517/99‑1 (MQ=255)                                                tCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATgag                                              <  1:689396/100‑1 (MQ=255)                                                 cGCATGAAGTAAATCATCGCTTGGCACAAAGTAGTGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATgaga                                             <  2:2058532/100‑1 (MQ=255)                                                 cGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATgaga                                             >  1:315267/1‑100 (MQ=255)                                                 cGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATgaga                                             <  1:689226/100‑1 (MQ=255)                                                  gCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGAt                                            <  1:2231816/100‑1 (MQ=255)                                                  gCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGAt                                            >  1:477884/1‑100 (MQ=255)                                                   cATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATc                                           >  1:1668330/1‑100 (MQ=255)                                                      gAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTc                                        <  2:1801969/100‑1 (MQ=255)                                                       aaGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTcc                                       <  2:2386743/100‑1 (MQ=255)                                                       aaGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTcc                                       >  1:301522/1‑100 (MQ=255)                                                        aGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCg                                      <  1:205317/100‑1 (MQ=255)                                                         gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                     >  1:747380/1‑100 (MQ=255)                                                         gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                     <  1:1300022/100‑1 (MQ=255)                                                         gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                     <  2:1833388/100‑1 (MQ=255)                                                             atcatcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAg                                 >  2:1614851/1‑100 (MQ=255)                                                              tcatcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGc                                <  1:1755768/100‑1 (MQ=255)                                                                atcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCg                              >  2:1046195/1‑100 (MQ=255)                                                                  cGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCggg                            <  1:2552291/100‑1 (MQ=255)                                                                  cGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCggg                            <  1:1581996/100‑1 (MQ=255)                                                                     ttGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGcc                         <  2:356397/100‑1 (MQ=255)                                                                      tGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCa                        >  2:558396/1‑100 (MQ=255)                                                                       ggCACAAAGCAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAg                       >  1:561804/1‑100 (MQ=255)                                                                        gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGACTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAg                       >  1:1373478/1‑99 (MQ=255)                                                                        gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                      >  1:1111566/1‑100 (MQ=255)                                                                        gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                      >  1:792633/1‑100 (MQ=255)                                                                        gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                      >  1:640004/1‑100 (MQ=255)                                                                         cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                     >  2:1239910/1‑100 (MQ=255)                                                                         cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                     >  2:625256/1‑100 (MQ=255)                                                                          acaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                    <  1:1125447/100‑1 (MQ=255)                                                                          acaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCATGGTGTATGAGATCATCCGGAAAGCCGGGGCCAGAAg                    >  2:1728867/1‑100 (MQ=255)                                                                            aaaGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                    <  2:2397062/98‑1 (MQ=255)                                                                             aaGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCaa                 >  1:728861/1‑100 (MQ=255)                                                                              aggaGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAAt                <  2:747373/97‑1 (MQ=255)                                                                              aGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAAt                >  2:1328794/1‑100 (MQ=255)                                                                               gTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAAtt               <  1:2229463/100‑1 (MQ=255)                                                                               gTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCGTCCGGAAAGCCGGGGCCAGAAGCAAt                <  2:523517/99‑1 (MQ=255)                                                                                tAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtg              <  2:765477/100‑1 (MQ=255)                                                                                tAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtg              <  1:749937/100‑1 (MQ=255)                                                                                  gggCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg            <  1:1337118/100‑1 (MQ=255)                                                                                    gCGTGGTCATGAACAACTATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtgtg          <  2:1498793/100‑1 (MQ=255)                                                                                     cGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGa         <  2:568089/100‑1 (MQ=255)                                                                                     cGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGa         <  2:301518/100‑1 (MQ=255)                                                                                        ggTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATg      >  1:2334783/1‑100 (MQ=255)                                                                                         gTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATACCTAGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATg      >  2:457802/1‑99 (MQ=255)                                                                                          tCATGAACAAATATTCGCGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgt    <  2:1111733/100‑1 (MQ=255)                                                                                           cATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgtg   <  1:1906449/100‑1 (MQ=255)                                                                                           cATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgtg   >  2:1470101/1‑100 (MQ=255)                                                                                            aTGAACAAATTTTCACGCGCCCAATATGCCGCAGTCAATCATGCCAGGTGAATGAGATCTTCCGGAAAGCCGGTGCCAGAAGCAATTGTGTGAATGgtgt  <  2:871268/100‑1 (MQ=255)                                                                                                |                                                                                                CGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGT  >  NC_000913/1307539‑1307729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑