Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,311,830	A→G	18.7%	intergenic (+22/+18)	tonB → / ← yciA	membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,311,830	0	A	G	18.7%	163.7 / 30.7	81	intergenic (+22/+18)	tonB/yciA	membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
Reads supporting (aligned to +/- strand):  ref base A (23/42);  new base G (11/4);  total (35/46)
Fisher's exact test for biased strand distribution p-value = 9.75e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

ATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGC  >  NC_000913/1311732‑1311927                                                                                                   |                                                                                                  aTGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAc                                                                                                  <  1:550084/100‑1 (MQ=255)     gCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGa                                                                                              <  1:2318049/100‑1 (MQ=255)      ccGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAg                                                                                             <  2:1021428/100‑1 (MQ=255)         ggTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCt                                                                                          <  1:1330721/100‑1 (MQ=255)         ggTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCt                                                                                          >  1:1865182/1‑100 (MQ=255)           tAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCttt                                                                                        <  1:1208214/100‑1 (MQ=255)            aaGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCtttt                                                                                       <  1:929959/100‑1 (MQ=255)            aaGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCtttt                                                                                       <  2:1834955/100‑1 (MQ=255)            aaGCAAGGAAGTAGGATTGTGGTGAATATCATGTTTAAAATTACCGACACCACCGAGATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCtttt                                                                                       <  2:479842/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg                                                                                      <  2:2466591/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg                                                                                      <  2:305991/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTg                                                                                      <  2:1945192/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTg                                                                                      <  2:2356785/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTg                                                                                      <  2:210348/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTg                                                                                      <  2:691786/100‑1 (MQ=255)             aGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTg                                                                                      <  1:988828/100‑1 (MQ=255)               ccAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc                                                                                    >  1:903735/1‑100 (MQ=255)               ccAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc                                                                                    <  1:1101989/100‑1 (MQ=255)                cAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTg                                                                                      <  2:1224505/97‑1 (MQ=255)                   gCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTAtt                                                                                <  1:724965/100‑1 (MQ=255)                       tGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTc                                                                            <  1:685175/100‑1 (MQ=255)                       tGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTc                                                                            <  2:684840/100‑1 (MQ=255)                          gATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAAc                                                                         <  2:2511712/100‑1 (MQ=255)                              gtggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAAGCTTTTGACTATTACTCAACAGGt                                                                     >  2:143815/1‑100 (MQ=255)                               tggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTa                                                                    <  1:1200690/100‑1 (MQ=255)                               tcttgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTa                                                                    <  1:1694400/97‑1 (MQ=255)                                ggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGCCTCATACTCAACAGGTaa                                                                   >  2:33357/1‑100 (MQ=255)                                ggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTaa                                                                   >  1:131558/1‑100 (MQ=255)                                ggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCACAGGATTTACACTATTACTCAACAAGTaa                                                                   >  2:666858/1‑100 (MQ=255)                                ctcttccgatcTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTaa                                                                   <  1:1224129/90‑1 (MQ=255)                                 gtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAg                                                                  <  1:1695316/100‑1 (MQ=255)                                  tgAATATCCTGTTTAAAATTAACGGCACCACCGCAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAga                                                                 >  2:1694388/1‑99 (MQ=255)                                    aaTATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGgcg                                                               <  1:1670360/100‑1 (MQ=255)                                         ccTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAgg                                                          <  2:1970710/100‑1 (MQ=255)                                            gTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGttt                                                       >  1:364800/1‑100 (MQ=255)                                             tttAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGtttt                                                      >  1:1165123/1‑100 (MQ=255)                                              ttAAAGATAACGGGACCACCTAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTc                                                     <  2:1518103/100‑1 (MQ=255)                                               tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                    >  2:1866741/1‑100 (MQ=255)                                               tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                    <  1:1299906/100‑1 (MQ=255)                                               tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                    <  2:2526153/100‑1 (MQ=255)                                               tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                    <  2:131556/100‑1 (MQ=255)                                                aaaaTTAACTGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCt                                                   >  2:2254697/1‑100 (MQ=255)                                                aaaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCt                                                   <  2:909411/100‑1 (MQ=255)                                                 aaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCtt                                                  >  1:459871/1‑100 (MQ=255)                                                  aaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTc                                                 <  2:1772703/100‑1 (MQ=255)                                                    ttAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAg                                               <  2:994648/100‑1 (MQ=255)                                                      aaCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAACGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAAGCGCGAGGTTTTCCTTCAg                                               >  2:2190550/1‑98 (MQ=255)                                                         ggCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAg                                               <  2:2508902/95‑1 (MQ=255)                                                          gCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCaa                                         >  2:1947624/1‑100 (MQ=255)                                                            accaccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAAcc                                       >  1:109788/1‑100 (MQ=255)                                                              caccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcgc                                     <  2:1792999/100‑1 (MQ=255)                                                              caccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcgc                                     >  1:1334377/1‑100 (MQ=255)                                                                ccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcgcg                                    >  1:785425/1‑99 (MQ=255)                                                                 cGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGAc                                  <  2:740127/100‑1 (MQ=255)                                                                 cGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGAc                                  <  2:737973/100‑1 (MQ=255)                                                                 cGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGAc                                  <  2:1168722/100‑1 (MQ=255)                                                                  gAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa                                 >  1:1830349/1‑100 (MQ=255)                                                                     aTTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATAc                              >  1:1157364/1‑100 (MQ=255)                                                                      ttCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACt                             <  1:2295556/100‑1 (MQ=255)                                                                       tCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACt                             <  1:2294592/99‑1 (MQ=255)                                                                         aGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGCCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTa                           <  2:1342155/99‑1 (MQ=255)                                                                           tAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaat                        <  2:120149/100‑1 (MQ=255)                                                                            naGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaata                       >  2:1996623/2‑100 (MQ=255)                                                                            aaGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaata                       <  1:2169906/100‑1 (MQ=255)                                                                             aGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaataa                      >  1:2057073/1‑100 (MQ=255)                                                                              agtaataGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaataat                     >  2:120801/7‑100 (MQ=37)                                                                                  ataGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCtt                 >  1:412738/3‑100 (MQ=255)                                                                                   aaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTc                >  1:2070816/1‑100 (MQ=255)                                                                                   aaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTc                <  1:593958/100‑1 (MQ=255)                                                                                   aaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTc                <  2:67967/100‑1 (MQ=255)                                                                                     gTCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTg              >  1:780323/1‑100 (MQ=255)                                                                                      tCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGt             >  1:891580/1‑100 (MQ=255)                                                                                       cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGCTCAACCGCGACATACTTAAATAATGCTTATGTc            >  2:323003/1‑100 (MQ=38)                                                                                       cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc            >  1:1528470/1‑100 (MQ=255)                                                                                       cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTc            >  1:906898/1‑100 (MQ=255)                                                                                        aaaaGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCg           >  2:434530/1‑100 (MQ=255)                                                                                        aaaaGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCg           >  2:104340/1‑100 (MQ=255)                                                                                         aaaGCCTCCGTTCGGAACCTTTCGTCTATTACTCAACAGTTAAGGCGCGAGGCTTTACTTCAGGTTCAACCGCGACATACTTAAATAATGCTTCTGTCGc          >  2:738479/1‑100 (MQ=255)                                                                                         aaaGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGCCATACTTAAATAATGCTTCTGTCGc          >  2:293312/1‑100 (MQ=255)                                                                                         aaaGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGc          >  1:482221/1‑100 (MQ=255)                                                                                                 cGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGc  >  2:1933598/1‑100 (MQ=255)                                                                                                   |                                                                                                  ATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGC  >  NC_000913/1311732‑1311927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑