Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,434,459 | C→T | 100% | intergenic (‑235/+499) | ynaE ← / ← ttcC | cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,434,459 | 0 | C | T | 86.4% | 29.0 / 1.9 | 22 | intergenic (‑235/+499) | ynaE/ttcC | cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related |
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (10/9); total (11/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 |
CTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGC > NC_000913/1434363‑1434539 | cTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTgg < 1:2400742/100‑1 (MQ=25) tGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTggg > 1:258340/1‑100 (MQ=25) tGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTAATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTggg < 2:2117630/100‑1 (MQ=12) gAATATGATAAGTAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt > 1:1686127/1‑100 (MQ=255) gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt > 1:1223937/1‑100 (MQ=18) gAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt > 2:1274855/1‑100 (MQ=18) aaTATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgtg < 2:356903/100‑1 (MQ=14) aaTATGATAAGGAGCGAAGTGATTATCAGTATGATGTTCATATAGCCTCGAATTAGTAATGTGTTATATATTATATAGTTGACAATTGTTATCTTGGgtg > 2:1912985/1‑100 (MQ=11) tatGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGtt < 2:1605683/100‑1 (MQ=14) ntGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGtt > 1:1045563/2‑99 (MQ=25) atGATAAGGAGCGAAGTTATTATCAGTATGCTGTTCATATAGCCTGGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGgt < 2:2039572/96‑1 (MQ=255) atGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTc < 2:334255/100‑1 (MQ=18) tGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCt > 2:1692066/1‑100 (MQ=18) tGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCt < 1:492981/100‑1 (MQ=14) tAAGGAGGCAAGTGATTATCAGTCTGCTGCATATATAGCCACGAATTAGTAAAGTGTTATATATGACATCGTTGACAATTTTTTTTTTGGGTGTCCTTaa > 2:1941669/1‑100 (MQ=255) tAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTaa > 2:1061996/1‑100 (MQ=18) aGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAg < 1:551500/100‑1 (MQ=18) ggAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGt < 2:930395/100‑1 (MQ=14) cGAAGTGATTATCAGTATGCTGTTCATATAGCATCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCTTGGGTGTTCTTAAAGTTCGt > 1:523113/1‑100 (MQ=2) tATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCg > 1:1284561/1‑100 (MQ=33) ttATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTggcggc < 2:2023330/100‑1 (MQ=35) ataGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGc < 2:1284553/100‑1 (MQ=38) | CTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGC > NC_000913/1434363‑1434539 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |