Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,435,737	G→A	100%	intergenic (‑118/+23)	uspF ← / ← ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,435,737	0	G	A	92.3%	250.2 / 9.4	94	intergenic (‑118/+23)	uspF/ompN	stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base A (34/50);  total (40/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCT  >  NC_000913/1435641‑1435835                                                                                                 |                                                                                                   cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCccacca                                                                                                 <  2:579342/100‑1 (MQ=255) cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCccacca                                                                                                 <  1:637920/100‑1 (MQ=255) cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCccacca                                                                                                 >  2:1848220/1‑100 (MQ=255)  gCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAATCCCTCCAt                                                                                                >  1:377059/1‑100 (MQ=255)  gCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCAt                                                                                                >  1:1068914/1‑100 (MQ=255)  gCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCAt                                                                                                >  2:2048931/1‑100 (MQ=255)    aTAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCAt                                                                                                <  2:1554721/98‑1 (MQ=255)     tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGGAGAATAAGCAGAAAAGGCCCACCATa                                                                                               >  1:537633/1‑98 (MQ=255)     tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAAc                                                                                             >  1:233477/1‑100 (MQ=255)     tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAAc                                                                                             >  1:558890/1‑100 (MQ=255)     tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAAc                                                                                             >  1:909890/1‑100 (MQ=255)      aaaGTGTAACGCCAGATGACACTTTTTTTTTAATTTTGGAGTTCACATTTATAATGTATATCAAAGGAGGAAGAATAAGCAGAAAAAGACCCCCATAACa                                                                                            >  2:925984/1‑100 (MQ=255)      aaaGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACa                                                                                            <  2:1516144/100‑1 (MQ=255)          tgtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTTTTTTTTTTTTAAGAAGGAAAAATAAGCAGAAAAAGACCCTCATAACAggg                                                                                         >  2:469314/1‑97 (MQ=255)          tgtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCgg                                                                                        >  2:2323744/1‑100 (MQ=255)          tgtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCgg                                                                                        <  1:803860/100‑1 (MQ=255)           gtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCggg                                                                                       >  1:424145/1‑100 (MQ=255)             aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCa                                                                                     <  1:292696/100‑1 (MQ=255)                 ccAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCaggag                                                                                 <  1:2180557/100‑1 (MQ=255)                 ccAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCaggag                                                                                 <  1:1267387/100‑1 (MQ=255)                  cAGATGACACTTTTTGTGTAATGCCGGAGTTCACATTTTTAATTTAGATTAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCaggagg                                                                                >  1:2365956/1‑100 (MQ=255)                  cAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCaggagg                                                                                <  1:1867320/100‑1 (MQ=255)                      tGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGGGGAttt                                                                            <  1:1338712/100‑1 (MQ=255)                      tGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGAttt                                                                            >  2:182853/1‑100 (MQ=255)                      tGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGAttt                                                                            <  2:1479765/100‑1 (MQ=255)                       gACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTa                                                                           <  1:402859/100‑1 (MQ=255)                        acacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAg                                                                          <  2:2280188/100‑1 (MQ=255)                         cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGa                                                                         <  2:319000/100‑1 (MQ=255)                           ctgtttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAAc                                                                       >  2:1089571/4‑100 (MQ=255)                            tttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACt                                                                      <  2:1482305/100‑1 (MQ=255)                            tttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACt                                                                      <  1:918395/100‑1 (MQ=255)                             ttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTg                                                                     <  2:2088926/100‑1 (MQ=255)                               ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                   <  1:1606709/100‑1 (MQ=255)                               ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                   <  2:145767/100‑1 (MQ=255)                               ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                   >  1:944110/1‑100 (MQ=255)                               ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGACCGCCATAACAGCGGGCAGGAGGATTTAGAACTg                                                                     >  2:917770/1‑98 (MQ=255)                                 gtgtAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATaa                                                                 >  2:1231627/1‑100 (MQ=255)                                   gtAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                               >  1:1835017/1‑100 (MQ=255)                                    taagtaCGAAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAcc                                                              <  2:2387242/95‑1 (MQ=255)                                    tAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATTAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAcc                                                              <  2:1499888/100‑1 (MQ=255)                                      aTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAg                                                            >  1:2102416/1‑100 (MQ=255)                                       tGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGa                                                           >  2:725486/1‑100 (MQ=255)                                        gACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc                                                          <  1:1959373/100‑1 (MQ=255)                                        gACGGAGATCACATTTTTAATTTAGATCAAAGGAGGAAGAAAAAAAAAAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc                                                          <  2:2150608/100‑1 (MQ=255)                                         aCGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc                                                         >  1:1599621/1‑100 (MQ=255)                                          cGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCt                                                        >  2:1796440/1‑100 (MQ=255)                                          cGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCt                                                        >  1:989944/1‑100 (MQ=255)                                           ggAGTTCGCATTTTTTAGGTAGATGGAAGGGGGAAGAAGAAGGAGAGAAAGCCCACCATAACGGCGGGCAGGAGGATTTGGAACTGATAAACCAggc                                                          <  2:1682250/97‑3 (MQ=255)                                           ggAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTa                                                       >  2:1601719/1‑100 (MQ=255)                                              gTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAg                                                    <  2:2016136/100‑1 (MQ=255)                                               ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                   <  1:2247601/100‑1 (MQ=255)                                                tCAAATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCATTATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                   <  2:203246/99‑1 (MQ=255)                                                  acaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCCGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGAc                                                <  2:1414105/100‑1 (MQ=255)                                                  acaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGAc                                                <  2:1599610/100‑1 (MQ=255)                                                  acaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGAc                                                >  1:128011/1‑100 (MQ=255)                                                   caTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                               <  2:1883448/100‑1 (MQ=255)                                                   caTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                               <  1:2138543/100‑1 (MQ=255)                                                   caTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                               >  1:1699920/1‑100 (MQ=255)                                                   caTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                               >  2:443273/1‑100 (MQ=255)                                                    aTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaa                                              <  2:1859604/100‑1 (MQ=255)                                                         tAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatca                                         <  1:266713/100‑1 (MQ=255)                                                          aaTTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcat                                        >  1:589337/1‑100 (MQ=255)                                                           aTTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcatc                                       <  1:1445930/100‑1 (MQ=255)                                                            tttAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCg                                      >  1:2199199/1‑100 (MQ=255)                                                              tAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGt                                    <  2:388042/100‑1 (MQ=255)                                                                 aTCAAAGGAGGAAGAATAAGTAGAAAAAGCTCATCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGATCTAAAGCGACAAGATCATCGGTaga                                 <  2:1794946/100‑1 (MQ=255)                                                                  tCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTagag                                >  1:1248415/1‑100 (MQ=255)                                                                    aaaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAt                              <  2:1633524/100‑1 (MQ=255)                                                                    aaaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAt                              <  2:29471/100‑1 (MQ=255)                                                                    aaaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAt                              <  1:1721767/100‑1 (MQ=255)                                                                     aaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATg                             <  1:2034944/100‑1 (MQ=255)                                                                       ggaggaAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGcc                           <  1:5360/100‑1 (MQ=255)                                                                          ggaAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCAtt                        <  1:1224078/100‑1 (MQ=255)                                                                           gaagaaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTg                       <  2:1462974/100‑1 (MQ=255)                                                                           gaagaaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTg                       <  2:2421738/100‑1 (MQ=255)                                                                            aagaaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTgg                      <  1:828200/100‑1 (MQ=255)                                                                             agaaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGc                     <  1:302204/100‑1 (MQ=255)                                                                              gaaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGAATTTAGACCTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCa                    <  2:2235001/100‑1 (MQ=255)                                                                               aaTAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                   <  2:377055/100‑1 (MQ=255)                                                                               aaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                   <  2:63870/100‑1 (MQ=255)                                                                                aTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGc                  >  1:889077/1‑100 (MQ=255)                                                                                 tAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCg                 <  1:517737/100‑1 (MQ=255)                                                                                 tAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAAGCCATTGGCAGct                 >  2:2183657/1‑99 (MQ=255)                                                                                  aaGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt                <  1:303610/100‑1 (MQ=255)                                                                                  aaGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt                >  2:527685/1‑100 (MQ=255)                                                                                  aaGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt                >  1:2105337/1‑100 (MQ=255)                                                                                   aGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa               >  1:141498/1‑100 (MQ=255)                                                                                   aGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa               <  1:593145/100‑1 (MQ=255)                                                                                   aGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa               <  2:1866731/100‑1 (MQ=255)                                                                                    gCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg              >  2:579092/1‑100 (MQ=255)                                                                                      aGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGTTAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa            <  2:1638964/100‑1 (MQ=255)                                                                                      aGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGaa            >  2:625711/1‑100 (MQ=255)                                                                                           aaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGt       <  2:1076909/100‑1 (MQ=255)                                                                                            aGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTc      <  1:1520697/100‑1 (MQ=255)                                                                                              cccACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCAt    >  2:1861215/1‑100 (MQ=255)                                                                                               ccGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATc   >  2:673973/1‑100 (MQ=255)                                                                                                cGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCt  >  1:782420/1‑100 (MQ=255)                                                                                                 |                                                                                                   CGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCATCT  >  NC_000913/1435641‑1435835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑