Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,482,227 C→A 16.1% intergenic (+26/+28) ynbD → / ← azoR putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,482,2270CA16.1% 264.9 / 35.1 98intergenic (+26/+28)ynbD/azoRputative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base C (42/40);  new base A (10/6);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 101 total aligned reads displayed.

TTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCG  >  NC_000913/1482128‑1482324
                                                                                                   |                                                                                                 
ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATTTGGACTGAATATc                                                                                                   <  2:1578765/100‑1 (MQ=255)
ttCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATc                                                                                                   <  1:442860/100‑1 (MQ=255)
  cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                 >  1:2292957/1‑100 (MQ=255)
  cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                 >  2:2107307/1‑100 (MQ=255)
  cGAGCCAGACGCCCGCAGATTGTACTGACAGACGAGCACAAAGCGATGCTGAGATTCTGGGAAAACAGGTAAGGGGATTGAAATGTGGACTAAATATCTa                                                                                                 >  2:2245254/1‑100 (MQ=255)
   gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGACAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                                >  2:2555075/1‑100 (MQ=255)
   gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                                <  2:258506/100‑1 (MQ=255)
     gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                              <  2:1709640/100‑1 (MQ=255)
       cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                            <  1:1206274/100‑1 (MQ=255)
        aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGGTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTcc                                                                                           >  1:2354037/1‑100 (MQ=255)
        aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                           <  1:1055096/100‑1 (MQ=255)
         gACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                          <  1:36858/100‑1 (MQ=255)
           cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                        <  1:578683/100‑1 (MQ=38)
            gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                       <  2:878453/100‑1 (MQ=38)
             cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                      <  1:2150101/100‑1 (MQ=38)
             cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                      <  1:2087245/100‑1 (MQ=255)
              ttccgatctgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTACGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                     <  1:757945/92‑1 (MQ=255)
                  aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGTGATTATGTGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                      <  2:1823292/95‑1 (MQ=37)
                  aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                 <  2:171943/100‑1 (MQ=255)
                  aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCAACAGTCCACATCAAGAc                                                                                 <  2:156804/100‑1 (MQ=255)
                    aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatcc                                                                               <  2:292665/100‑8 (MQ=25)
                    aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatc                                                                                <  1:2312393/99‑7 (MQ=25)
                    aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCg                                                                               <  1:1271525/100‑1 (MQ=255)
                      tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                             <  1:1018035/100‑1 (MQ=255)
                       gtgCTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACGGAATATCTACAGTCCACATCAAGataggaa                                                                            >  2:757938/1‑94 (MQ=255)
                        tgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                           >  2:1972023/1‑100 (MQ=255)
                        tgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                           <  1:2295585/100‑1 (MQ=255)
                          cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                         >  2:1847857/1‑100 (MQ=255)
                          cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                         >  2:605171/1‑100 (MQ=255)
                           tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                        >  2:270969/1‑100 (MQ=255)
                            gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                       <  2:1328704/100‑1 (MQ=255)
                            gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                       <  2:1598073/100‑1 (MQ=255)
                            gACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                       <  2:807871/100‑1 (MQ=255)
                              cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                     <  1:548442/100‑1 (MQ=255)
                              cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                     <  2:1959401/100‑1 (MQ=255)
                              cAGACGAGCACAAAGCGATGCTGAGATTAGGGGAAATCAGGTAAGGGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                     <  2:1741900/100‑1 (MQ=255)
                                gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                   >  1:2342380/1‑100 (MQ=255)
                                gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                   <  1:2313862/100‑1 (MQ=255)
                                    aGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGa                                                               >  1:2205928/1‑100 (MQ=255)
                                     gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATATACAGTCCACATCAAGACCGTGTCCGGATATGCAGaa                                                              >  2:368293/1‑100 (MQ=255)
                                       acaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAAc                                                            >  1:2132811/1‑100 (MQ=255)
                                        caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                           >  1:1305656/1‑100 (MQ=255)
                                          aaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                         >  1:2228347/1‑100 (MQ=255)
                                            gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                       <  2:14113/100‑1 (MQ=255)
                                            gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                       <  2:2246830/100‑1 (MQ=255)
                                              gATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTg                                                     <  1:206618/100‑1 (MQ=255)
                                                ttcttagaTTATATTAAAACATTTAAATCTATTGATATGTTTACTTAATATCTACATTCCACATCAATACCGTGTCCGGTTATGCATaa                                                              <  2:140974/84‑1 (MQ=255)
                                                tGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTc                                                   <  1:323175/100‑1 (MQ=255)
                                                 gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                                  >  1:2106141/1‑100 (MQ=255)
                                                  cTGAGATTATGGGAAAACAGGTATGTGGACTGAGATGTGGTCTGAATAACTACAGTCCACATCAGGACCGTGTCCGGTTATACAGAAACAATgataccga                                                 >  2:2114313/1‑93 (MQ=255)
                                                  cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                 >  1:1577315/1‑100 (MQ=255)
                                                  cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                 >  1:2499450/1‑100 (MQ=255)
                                                  cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                 >  1:2087079/1‑100 (MQ=255)
                                                   tGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGAt                                                <  1:1279440/100‑1 (MQ=255)
                                                      gaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgc                                             <  2:632063/100‑1 (MQ=255)
                                                        taaTGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctg                                           >  1:2365320/3‑100 (MQ=255)
                                                           tGGGAAAACGGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTGCGGTTATGCAGAAACAATGCTGTCGATGgc                                             >  2:759913/1‑95 (MQ=255)
                                                           tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                        >  1:128562/1‑100 (MQ=255)
                                                            gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                       >  2:222491/1‑100 (MQ=255)
                                                              gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTg                                     <  2:465116/100‑1 (MQ=255)
                                                                aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                   <  2:1445509/100‑1 (MQ=255)
                                                                aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                   >  1:666574/1‑100 (MQ=255)
                                                                    aGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAg                               <  2:1389325/100‑1 (MQ=255)
                                                                     ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCGACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCaaa                              >  2:1906641/1‑98 (MQ=255)
                                                                     ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                              >  1:2094191/1‑100 (MQ=255)
                                                                         acacggtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                          >  2:843638/11‑100 (MQ=21)
                                                                         aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                          >  2:649487/1‑100 (MQ=255)
                                                                         aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                          >  1:1170956/1‑100 (MQ=255)
                                                                           tGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGc                        <  1:552256/100‑1 (MQ=255)
                                                                            ggATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGc                        >  2:12814/1‑99 (MQ=255)
                                                                            cggtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCt                       >  2:2309797/8‑100 (MQ=25)
                                                                              gtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                     >  1:1404499/6‑100 (MQ=25)
                                                                              aTTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                     >  1:1929096/1‑100 (MQ=255)
                                                                               ttGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGGCAGACTGTGCTTTc                    >  2:1655432/1‑100 (MQ=255)
                                                                                 gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc                  <  1:1918464/100‑1 (MQ=255)
                                                                                   gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                <  2:1221778/100‑1 (MQ=255)
                                                                                    aTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTtgctgc               >  2:2335160/1‑100 (MQ=38)
                                                                                    aTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc               >  1:1949985/1‑100 (MQ=38)
                                                                                    aTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc               >  2:469208/1‑100 (MQ=38)
                                                                                    aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc               <  2:2488648/100‑1 (MQ=255)
                                                                                    aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc               <  1:2005452/100‑1 (MQ=255)
                                                                                     tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc              <  1:1909390/100‑1 (MQ=255)
                                                                                     tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc              <  1:1835429/100‑1 (MQ=255)
                                                                                      gtgGACTGTAGATATTCAGTCCACATCTAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCa             >  1:816428/1‑100 (MQ=38)
                                                                                      gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc               >  2:947696/1‑98 (MQ=38)
                                                                                        ggACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt           >  1:319398/1‑100 (MQ=255)
                                                                                        ggACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt           >  1:1461472/1‑100 (MQ=255)
                                                                                        ggACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt           >  2:2537362/1‑100 (MQ=255)
                                                                                          aCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGATTTCGCTGCCATTTc         >  1:2519360/1‑100 (MQ=255)
                                                                                           cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc        >  2:1840967/1‑100 (MQ=255)
                                                                                            tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg       <  1:53158/100‑1 (MQ=255)
                                                                                            tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg       <  1:1810232/100‑1 (MQ=255)
                                                                                             gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg      <  1:834217/100‑1 (MQ=255)
                                                                                             gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg      >  1:2295071/1‑100 (MQ=255)
                                                                                              aaTATCTGCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCCTCAGACTGTGCTTTCGCTGCCATTTc         >  2:714513/1‑96 (MQ=255)
                                                                                               atatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTAAGACTGTGCTTTCGCTGCCATTTCCGGAc    >  2:242461/1‑100 (MQ=255)
                                                                                                tatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCAGAcc   >  1:2079258/1‑100 (MQ=255)
                                                                                                 atCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg  >  1:1345158/1‑100 (MQ=255)
                                                                                                 atCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg  >  1:2115640/1‑100 (MQ=255)
                                                                                                   cTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg  <  1:1186890/98‑1 (MQ=255)
                                                                                                   |                                                                                                 
TTCGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCG  >  NC_000913/1482128‑1482324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: