Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,482,229	A→T	15.8%	intergenic (+28/+26)	ynbD → / ← azoR	putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,482,229	0	A	T	15.8%	239.6 / 32.6	96	intergenic (+28/+26)	ynbD/azoR	putative phosphatase inner membrane protein/NADH‑azoreductase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (39/41);  new base T (9/6);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

CGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATG  >  NC_000913/1482130‑1482328                                                                                                    |                                                                                                    cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                     >  2:2107307/1‑100 (MQ=255) cGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTa                                                                                                     >  1:2292957/1‑100 (MQ=255) cGAGCCAGACGCCCGCAGATTGTACTGACAGACGAGCACAAAGCGATGCTGAGATTCTGGGAAAACAGGTAAGGGGATTGAAATGTGGACTAAATATCTa                                                                                                     >  2:2245254/1‑100 (MQ=255)  gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGACAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                                    >  2:2555075/1‑100 (MQ=255)  gAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTAc                                                                                                    <  2:258506/100‑1 (MQ=255)    gCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAg                                                                                                  <  2:1709640/100‑1 (MQ=255)      cAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTc                                                                                                <  1:1206274/100‑1 (MQ=255)       aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGGTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTcc                                                                                               >  1:2354037/1‑100 (MQ=255)       aGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTcc                                                                                               <  1:1055096/100‑1 (MQ=255)        gACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCca                                                                                              <  1:36858/100‑1 (MQ=255)          cGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCcaca                                                                                            <  1:578683/100‑1 (MQ=38)           gCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACAt                                                                                           <  2:878453/100‑1 (MQ=38)            cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                          <  1:2150101/100‑1 (MQ=38)            cccGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATc                                                                                          <  1:2087245/100‑1 (MQ=255)             ttccgatctgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTACGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCa                                                                                         <  1:757945/92‑1 (MQ=255)                 aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGTGATTATGTGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATc                                                                                          <  2:1823292/95‑1 (MQ=37)                 aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAc                                                                                     <  2:171943/100‑1 (MQ=255)                 aGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCAACAGTCCACATCAAGAc                                                                                     <  2:156804/100‑1 (MQ=255)                  gATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGAcc                                                                                    <  2:986846/100‑1 (MQ=255)                   aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatcc                                                                                   <  2:292665/100‑8 (MQ=25)                   aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGTAGATATTCAGTCCACATctcaatc                                                                                    <  1:2312393/99‑7 (MQ=25)                   aTTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCg                                                                                   <  1:1271525/100‑1 (MQ=255)                     tgtgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCgtg                                                                                 <  1:1018035/100‑1 (MQ=255)                      gtgCTGACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACGGAATATCTACAGTCCACATCAAGataggaa                                                                                >  2:757938/1‑94 (MQ=255)                       tgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                               >  2:1972023/1‑100 (MQ=255)                       tgCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTc                                                                               <  1:2295585/100‑1 (MQ=255)                         cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                             >  2:1847857/1‑100 (MQ=255)                         cTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCg                                                                             >  2:605171/1‑100 (MQ=255)                          tGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCgg                                                                            >  2:270969/1‑100 (MQ=255)                           gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                           <  2:1328704/100‑1 (MQ=255)                           gACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                           <  2:1598073/100‑1 (MQ=255)                           gACAGACGAGCACAAAGCGATGCTGAGAGTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGt                                                                           <  2:807871/100‑1 (MQ=255)                             cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                         <  1:548442/100‑1 (MQ=255)                             cAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                         <  2:1959401/100‑1 (MQ=255)                             cAGACGAGCACAAAGCGATGCTGAGATTAGGGGAAATCAGGTAAGGGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTa                                                                         <  2:1741900/100‑1 (MQ=255)                               gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                       <  1:2313862/100‑1 (MQ=255)                               gACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATg                                                                       >  1:2342380/1‑100 (MQ=255)                                   aGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGa                                                                   >  1:2205928/1‑100 (MQ=255)                                    gCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATATACAGTCCACATCAAGACCGTGTCCGGATATGCAGaa                                                                  >  2:368293/1‑100 (MQ=255)                                      acaAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAAc                                                                >  1:2132811/1‑100 (MQ=255)                                       caAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACa                                                               >  1:1305656/1‑100 (MQ=255)                                         aaGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAAt                                                             >  1:2228347/1‑100 (MQ=255)                                           gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                           <  2:2246830/100‑1 (MQ=255)                                           gCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGc                                                           <  2:14113/100‑1 (MQ=255)                                             gATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTg                                                         <  1:206618/100‑1 (MQ=255)                                               ttcttagaTTATATTAAAACATTTAAATCTATTGATATGTTTACTTAATATCTACATTCCACATCAATACCGTGTCCGGTTATGCATaa                                                                  <  2:140974/84‑1 (MQ=255)                                               tGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTc                                                       <  1:323175/100‑1 (MQ=255)                                                gCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCg                                                      >  1:2106141/1‑100 (MQ=255)                                                 cTGAGATTATGGGAAAACAGGTATGTGGACTGAGATGTGGTCTGAATAACTACAGTCCACATCAGGACCGTGTCCGGTTATACAGAAACAATgataccga                                                     >  2:2114313/1‑93 (MQ=255)                                                 cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                     >  1:2499450/1‑100 (MQ=255)                                                 cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                     >  1:1577315/1‑100 (MQ=255)                                                 cTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGa                                                     >  1:2087079/1‑100 (MQ=255)                                                  tGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGAt                                                    <  1:1279440/100‑1 (MQ=255)                                                     gaTTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgc                                                 <  2:632063/100‑1 (MQ=255)                                                       taaTGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGgctg                                               >  1:2365320/3‑100 (MQ=255)                                                          tGGGAAAACGGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTGCGGTTATGCAGAAACAATGCTGTCGATGgc                                                 >  2:759913/1‑95 (MQ=255)                                                          tGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCtt                                            >  1:128562/1‑100 (MQ=255)                                                           gggAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCttt                                           >  2:222491/1‑100 (MQ=255)                                                             gAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTg                                         <  2:465116/100‑1 (MQ=255)                                                               aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                       <  2:1445509/100‑1 (MQ=255)                                                               aaaCAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCg                                       >  1:666574/1‑100 (MQ=255)                                                                   aGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAg                                   <  2:1389325/100‑1 (MQ=255)                                                                    ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCGACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCaaa                                  >  2:1906641/1‑98 (MQ=255)                                                                    ggTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGa                                  >  1:2094191/1‑100 (MQ=255)                                                                        acacggtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                              >  2:843638/11‑100 (MQ=21)                                                                        aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                              >  2:649487/1‑100 (MQ=255)                                                                        aGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACtgt                              >  1:1170956/1‑100 (MQ=255)                                                                          tGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGc                            <  1:552256/100‑1 (MQ=255)                                                                           ggATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGc                            >  2:12814/1‑99 (MQ=255)                                                                           cggtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCt                           >  2:2309797/8‑100 (MQ=25)                                                                             gtcttgaTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                         >  1:1404499/6‑100 (MQ=25)                                                                             aTTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCttt                         >  1:1929096/1‑100 (MQ=255)                                                                              ttGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGGCAGACTGTGCTTTc                        >  2:1655432/1‑100 (MQ=255)                                                                                gagaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGc                      <  1:1918464/100‑1 (MQ=255)                                                                                  gaTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTg                    <  2:1221778/100‑1 (MQ=255)                                                                                   aTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTtgctgc                   >  2:2335160/1‑100 (MQ=38)                                                                                   aTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                   >  1:1949985/1‑100 (MQ=38)                                                                                   aTGTGGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                   >  2:469208/1‑100 (MQ=38)                                                                                   aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                   <  1:2005452/100‑1 (MQ=255)                                                                                   aTGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                   <  2:2488648/100‑1 (MQ=255)                                                                                    tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc                  <  1:1909390/100‑1 (MQ=255)                                                                                    tgtgGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGcc                  <  1:1835429/100‑1 (MQ=255)                                                                                     gtgGACTGTAGATATTCAGTCCACATCTAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCa                 >  1:816428/1‑100 (MQ=38)                                                                                     gtgGACTGTAGATATTCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGc                   >  2:947696/1‑98 (MQ=38)                                                                                       ggACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt               >  1:319398/1‑100 (MQ=255)                                                                                       ggACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt               >  1:1461472/1‑100 (MQ=255)                                                                                       ggACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCAtt               >  2:2537362/1‑100 (MQ=255)                                                                                         aCTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGATTTCGCTGCCATTTc             >  1:2519360/1‑100 (MQ=255)                                                                                          cTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTcc            >  2:1840967/1‑100 (MQ=255)                                                                                           tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg           <  1:53158/100‑1 (MQ=255)                                                                                           tGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCg           <  1:1810232/100‑1 (MQ=255)                                                                                            gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg          >  1:2295071/1‑100 (MQ=255)                                                                                            gAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCgg          <  1:834217/100‑1 (MQ=255)                                                                                             aaTATCTGCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCCTCAGACTGTGCTTTCGCTGCCATTTc             >  2:714513/1‑96 (MQ=255)                                                                                              atatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTAAGACTGTGCTTTCGCTGCCATTTCCGGAc        >  2:242461/1‑100 (MQ=255)                                                                                               tatCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCAGAcc       >  1:2079258/1‑100 (MQ=255)                                                                                                atCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg      >  1:1345158/1‑100 (MQ=255)                                                                                                atCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg      >  1:2115640/1‑100 (MQ=255)                                                                                                  cTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCg      <  1:1186890/98‑1 (MQ=255)                                                                                                    aCAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATg  <  2:810678/100‑1 (MQ=255)                                                                                                    |                                                                                                    CGAGCCAGACGCCCGCAGATTGTGCTGACAGACGAGCACAAAGCGATGCTGAGATTATGGGAAAACAGGTAAGTGGATTGAGATGTGGACTGAATATCTACAGTCCACATCAAGACCGTGTCCGGTTATGCAGAAACAATGCTGTCGATGGCTGCTTTTGCGTCAGACTGTGCTTTCGCTGCCATTTCCGGACCGTATG  >  NC_000913/1482130‑1482328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑