Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,533,713 A→C 15.2% F47V (TTT→GTT)  yddH ← flavin reductase like‑protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,533,7130AC15.2% 231.8 / 37.9 99F47V (TTT→GTT) yddHflavin reductase like‑protein
Reads supporting (aligned to +/- strand):  ref base A (46/38);  new base C (9/6);  total (55/44)
Fisher's exact test for biased strand distribution p-value = 7.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-02

Only 100 of 102 total aligned reads displayed.

CAACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACG  >  NC_000913/1533614‑1533812
                                                                                                   |                                                                                                   
cAACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCaaa                                                                                                     <  2:1319916/100‑1 (MQ=255)
 aaCGCCCGGGATAACTATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAAc                                                                                                    <  1:2515029/100‑1 (MQ=255)
 aaCGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAAc                                                                                                    <  2:359838/100‑1 (MQ=255)
   cGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTc                                                                                                  >  2:1171739/1‑100 (MQ=255)
      ccGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGGTTTATCTACCACAATCGTCACGCGTGGAGGTTCAAACGCCAc                                                                                               >  1:1395708/1‑100 (MQ=255)
      ccGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCAc                                                                                               >  1:1987663/1‑100 (MQ=255)
       cgcgATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGATTTTGAACCTCCAcg                                                                                              <  2:442053/97‑2 (MQ=255)
       cGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcg                                                                                              <  1:1001/100‑2 (MQ=255)
       cGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcg                                                                                              <  1:2521129/100‑2 (MQ=255)
         ggATAACGATGCCAAATTTACCGTTATGTTCAATCATCTCCCTGGTCCATTTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcg                                                                                            <  2:1351685/100‑4 (MQ=255)
         ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcg                                                                                            <  1:1767420/100‑4 (MQ=255)
         ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCgg                                                                                            <  1:1307508/100‑1 (MQ=255)
         ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCgg                                                                                            <  1:1681941/100‑1 (MQ=255)
          gATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGc                                                                                           >  2:1623386/1‑100 (MQ=255)
           aTAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCGATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCa                                                                                          <  2:1665491/100‑1 (MQ=255)
            tAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCAt                                                                                         <  1:96233/100‑1 (MQ=255)
            tAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAAGCGCCACGCGTGGAGTTTGAACCTCCAcgcgtgg                                                                                         <  2:2494729/100‑7 (MQ=255)
              aCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCAt                                                                                         >  2:1548907/1‑98 (MQ=255)
              aCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTg                                                                                       <  2:25595/100‑1 (MQ=255)
               cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACTACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa                                                                                      >  2:2129353/1‑100 (MQ=255)
               cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa                                                                                      >  1:524908/1‑100 (MQ=255)
               cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa                                                                                      <  1:1456136/100‑1 (MQ=255)
               cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa                                                                                      >  1:1401579/1‑100 (MQ=255)
                    ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg                                                                                 >  1:262034/1‑100 (MQ=255)
                    ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg                                                                                 >  1:1598966/1‑100 (MQ=255)
                     cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                >  1:2281543/1‑100 (MQ=255)
                       aaTTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTg                                                                              <  2:205032/100‑1 (MQ=255)
                        aTTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCTATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                <  2:1612085/97‑1 (MQ=255)
                         tttACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCa                                                                            <  2:1877284/100‑1 (MQ=255)
                           tACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGc                                                                          <  1:940154/100‑1 (MQ=255)
                             ccGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCa                                                                        >  2:1404436/1‑100 (MQ=255)
                               gTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATa                                                                      <  2:862129/100‑1 (MQ=255)
                               gTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATa                                                                      <  1:2011036/100‑1 (MQ=255)
                                 tATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAAt                                                                    >  1:1269576/1‑100 (MQ=255)
                                 tATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAAt                                                                    >  2:1549218/1‑100 (MQ=255)
                                    gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 >  1:2231466/1‑100 (MQ=255)
                                    gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 >  2:2294822/1‑100 (MQ=255)
                                    gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 >  1:2382301/1‑100 (MQ=255)
                                    gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 <  2:1297988/100‑1 (MQ=255)
                                         atcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAcgcc                                                            >  1:213210/1‑100 (MQ=255)
                                            aaCTCCCTGGTCCATGTCGATTTATCTACCACAATCCCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAccccgc                                                          >  2:634188/1‑99 (MQ=255)
                                             aCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATCATGTTACGCCGCTg                                                        <  2:756205/100‑1 (MQ=255)
                                             aCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTg                                                        <  1:1634430/100‑1 (MQ=255)
                                              cTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTg                                                        <  2:368122/99‑1 (MQ=255)
                                               tCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTggg                                                      >  1:1372437/1‑100 (MQ=255)
                                               tCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTggg                                                      >  1:106950/1‑100 (MQ=255)
                                                cccTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGa                                                     >  2:2255318/1‑100 (MQ=255)
                                                   tGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTg                                                  >  2:655400/1‑100 (MQ=255)
                                                    ggTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGc                                                 <  2:415794/100‑1 (MQ=255)
                                                     gTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCt                                                <  2:5588/100‑1 (MQ=255)
                                                     gTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCt                                                <  2:2096229/100‑1 (MQ=255)
                                                        cATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCAt                                             >  1:73457/1‑100 (MQ=255)
                                                            tCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGaa                                         >  1:1576416/1‑100 (MQ=255)
                                                             cGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGaaa                                        >  2:1454774/1‑100 (MQ=255)
                                                              gATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAAc                                       >  1:1350333/1‑100 (MQ=255)
                                                              gATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAAc                                       <  1:639204/100‑1 (MQ=255)
                                                                 ttATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTgg                                    <  2:831668/100‑1 (MQ=255)
                                                                    tCTACCACAACCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGa                                 <  1:2114075/100‑1 (MQ=255)
                                                                     cTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGAt                                >  2:444755/1‑100 (MQ=255)
                                                                      tACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATc                               >  1:626378/1‑100 (MQ=255)
                                                                      tACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATc                               >  1:1897714/1‑100 (MQ=255)
                                                                       aCCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCa                              <  2:262031/100‑1 (MQ=255)
                                                                        ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa                             >  2:2508555/1‑100 (MQ=255)
                                                                        ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa                             >  1:795759/1‑100 (MQ=255)
                                                                        ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa                             >  1:1126845/1‑100 (MQ=255)
                                                                        ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa                             >  1:796266/1‑100 (MQ=255)
                                                                          acaATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGa                           >  2:1925838/1‑100 (MQ=255)
                                                                           caATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGAc                          <  2:186695/100‑1 (MQ=255)
                                                                           caATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGAc                          <  2:626371/100‑1 (MQ=255)
                                                                            aaTCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGAcc                         <  1:701915/100‑1 (MQ=255)
                                                                             aTCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCg                        >  1:92579/1‑100 (MQ=255)
                                                                                gCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTg                     <  1:1430441/100‑1 (MQ=255)
                                                                                gCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTg                     >  1:377066/1‑100 (MQ=255)
                                                                                aatgccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGtt                      >  2:2000680/8‑99 (MQ=38)
                                                                                  cACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTggg                   >  1:1301389/1‑100 (MQ=255)
                                                                                  cACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTggg                   <  2:2484194/100‑1 (MQ=255)
                                                                                   gccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc                  >  2:1628467/5‑100 (MQ=255)
                                                                                   gccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc                  >  1:1328894/5‑100 (MQ=255)
                                                                                    ccggTGGAGTTTGAACCTCCACCGGCCTTGCCCAGGCTGCAGCCATAATGTTACGCCGATGGGATTGCTCATCGAAACTGGTGCTCAAGACCGTTGGGcc                 >  2:469584/4‑100 (MQ=255)
                                                                                    ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  2:744300/4‑100 (MQ=255)
                                                                                    ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  1:1465262/4‑100 (MQ=255)
                                                                                    ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  1:351599/4‑100 (MQ=255)
                                                                                    ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATAGAAACTGGTGATCAAGACCGTTGGGc                  >  2:2446427/4‑99 (MQ=255)
                                                                                      ggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTACTCATCGAAACTGGTGATCAAGACCGTTGGGCCAt               >  1:336194/2‑100 (MQ=255)
                                                                                      cgTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCa                >  2:564475/1‑99 (MQ=255)
                                                                                      cgTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCAt               <  1:895456/100‑1 (MQ=255)
                                                                                       gTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATg              <  2:2018871/100‑1 (MQ=255)
                                                                                         ggAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGAt            <  1:444759/100‑1 (MQ=255)
                                                                                          gAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGAtt           >  2:2486227/1‑100 (MQ=255)
                                                                                          gAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGAtt           <  1:2372067/100‑1 (MQ=255)
                                                                                            ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         <  2:299841/100‑1 (MQ=255)
                                                                                            ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         <  1:669235/100‑1 (MQ=255)
                                                                                            ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         >  1:164699/1‑100 (MQ=255)
                                                                                            ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         >  1:2092936/1‑100 (MQ=255)
                                                                                            ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGGTGGGCCATGAttt          >  2:650889/1‑99 (MQ=255)
                                                                                            ggTTCAAACTCCACCGGCATTGACCAGGCTGAAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         <  2:550868/100‑1 (MQ=255)
                                                                                               tCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACa      >  1:1306120/1‑100 (MQ=255)
                                                                                                cAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAg     >  2:1462025/1‑100 (MQ=255)
                                                                                                  aaCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGAc   <  2:1089570/100‑1 (MQ=255)
                                                                                                   aCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACg  <  1:2134535/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CAACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACG  >  NC_000913/1533614‑1533812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: