Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,324 A→G 100% S116P (TCT→CCT)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,3240AG98.0% 341.8 / ‑2.6 100S116P (TCT→CCT) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (53/45);  total (54/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 101 total aligned reads displayed.

TTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTT  >  NC_000913/1555227‑1555420
                                                                                                 |                                                                                                
tgaaTAGTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaa                                                                                                <  1:1110637/98‑1 (MQ=255)
   atatTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAAc                                                                                             >  1:562368/1‑100 (MQ=255)
    tattatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACg                                                                                            >  1:1045921/1‑100 (MQ=255)
    tattatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACg                                                                                            >  1:1155755/1‑100 (MQ=255)
     attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTACGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGc                                                                                           >  2:1032975/1‑100 (MQ=255)
     attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTACGAGATAAACACGCCGCGTGAACGGCGGTACATCTCAGGAAAAccc                                                                                           >  1:193592/1‑98 (MQ=255)
     attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCAGCGTGAACGGCGGTAGATCTCAGGAAAACGc                                                                                           >  1:1492624/1‑100 (MQ=255)
      ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCt                                                                                          >  2:2556383/1‑100 (MQ=255)
       tatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTc                                                                                         >  2:814184/1‑100 (MQ=255)
         tGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcac                                                                                       >  2:783782/1‑100 (MQ=255)
         tGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcac                                                                                       >  2:184666/1‑100 (MQ=255)
          ggTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcaca                                                                                      >  1:1384263/1‑100 (MQ=255)
          ggTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcaca                                                                                      <  1:1708159/100‑1 (MQ=255)
           gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAg                                                                                     >  1:2207760/1‑100 (MQ=255)
           gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAg                                                                                     >  2:320289/1‑100 (MQ=255)
             tCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGc                                                                                   <  2:556692/100‑1 (MQ=255)
              nggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCt                                                                                  >  1:2369417/2‑100 (MQ=255)
               ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                 >  1:1202683/1‑100 (MQ=255)
               ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                 >  1:945421/1‑100 (MQ=255)
               ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                 >  1:1935842/1‑100 (MQ=255)
               ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                 >  2:2539497/1‑100 (MQ=255)
                gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgc                                                                                >  2:630792/1‑100 (MQ=255)
                 cACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgcg                                                                               >  1:1772201/1‑100 (MQ=255)
                  aCGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgcgc                                                                              >  1:2164335/1‑100 (MQ=255)
                  aCGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgcgc                                                                              <  2:1812547/100‑1 (MQ=255)
                  aCGTTTGGCAGAATATCGTCCATAGTGTGCCGGTGCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGAGCTCAGGAAAACGCTCACAGGCTgcgc                                                                              <  2:2264399/100‑1 (MQ=255)
                   cGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGcc                                                                             <  2:654101/100‑1 (MQ=255)
                    gTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGcc                                                                             <  1:1687673/99‑1 (MQ=255)
                    gTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGcc                                                                             <  1:1012306/99‑1 (MQ=255)
                       ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATGTCAGGAAAACGCTCACAGGCTGCGCCGACg                                                                         >  2:2449500/1‑100 (MQ=255)
                       ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACg                                                                         >  2:1970981/1‑100 (MQ=255)
                        tggagaacgtCGTCCAGAGTGGGCCTGTTGTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCg                                                                            <  2:1670994/87‑1 (MQ=255)
                        tGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACgg                                                                        <  1:2333819/100‑1 (MQ=255)
                           aGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTg                                                                     <  2:1045913/100‑1 (MQ=255)
                                atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta                                                                >  1:1241391/1‑100 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATGAACACTCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              <  2:1727500/100‑1 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              >  1:966658/1‑100 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              >  2:814819/1‑100 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              <  1:2503912/100‑1 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              <  1:2031290/100‑1 (MQ=255)
                                  cGTCCATATTGTGCCGATTCTGGTAAGAGATACCCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              <  2:1492613/100‑1 (MQ=255)
                                    tCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaa                                                            <  1:2128455/100‑1 (MQ=255)
                                     ccATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaat                                                           >  2:299513/1‑100 (MQ=255)
                                         atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACa                                                       <  1:2130677/100‑1 (MQ=255)
                                          ttGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATTTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAg                                                      <  2:2094701/100‑1 (MQ=255)
                                           tgtgCCGGTTCTGGTAAGAGATAACCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCTACGGTTGGGGTATAAATAACa                                                       <  2:2134500/98‑1 (MQ=255)
                                            gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                    >  2:2279921/1‑100 (MQ=255)
                                            gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                    <  1:590562/100‑1 (MQ=255)
                                            gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                    >  2:2093846/1‑100 (MQ=255)
                                            gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                    >  1:2458190/1‑100 (MQ=255)
                                             tgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGca                                                   >  2:616103/1‑100 (MQ=255)
                                              gCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcat                                                  >  1:943176/1‑100 (MQ=255)
                                               ccGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatc                                                 >  1:1632159/1‑100 (MQ=255)
                                               ccGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatc                                                 >  1:877461/1‑100 (MQ=255)
                                                  gTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCatcatc                                              <  1:289445/100‑1 (MQ=255)
                                                  gTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCatcatc                                              >  1:750458/1‑100 (MQ=255)
                                                  gTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCatcatc                                              <  2:1498840/100‑1 (MQ=255)
                                                     cTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctca                                           >  1:1898606/1‑100 (MQ=255)
                                                      tGGTAAGAGATAAACACCCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                          <  2:2314174/100‑1 (MQ=255)
                                                       ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                         <  1:783789/100‑1 (MQ=255)
                                                       ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                         >  1:1446354/1‑100 (MQ=255)
                                                        gTAAGAGATAAACACGCCGCGTGAACGGCGTTAGTTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                        <  2:1598683/100‑1 (MQ=255)
                                                        gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                        <  1:1970995/100‑1 (MQ=255)
                                                            gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                    <  1:442261/100‑1 (MQ=255)
                                                            gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                    <  2:1888567/100‑1 (MQ=255)
                                                             agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatg                                   <  1:1544280/100‑1 (MQ=255)
                                                             agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatg                                   <  1:2498731/100‑1 (MQ=255)
                                                              gaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatga                                  >  1:1354202/1‑100 (MQ=255)
                                                                tAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                >  1:663530/1‑100 (MQ=255)
                                                                tAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                >  2:490762/1‑100 (MQ=255)
                                                                tAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                >  2:1931109/1‑100 (MQ=255)
                                                                  aaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAGGATGATTg                               <  2:160214/99‑1 (MQ=255)
                                                                  aaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg                               <  2:1189380/99‑1 (MQ=255)
                                                                  aaCACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGt                              >  1:2286353/1‑100 (MQ=255)
                                                                    cacGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGttt                            <  1:2556399/100‑1 (MQ=255)
                                                                     acGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAACTATCAGGCATCATCTCATCAAGATGATTGTTTa                           <  2:1690098/100‑1 (MQ=255)
                                                                     acGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTa                           <  2:2246748/100‑1 (MQ=255)
                                                                     acGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTa                           <  2:1659132/100‑1 (MQ=255)
                                                                       gccgcGTGAACGGCGGTAGATCTCAGGAAACCGCGCACAGGCTCCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                         <  2:2035515/100‑1 (MQ=255)
                                                                       gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                         <  1:636014/100‑1 (MQ=255)
                                                                       gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                         <  2:334804/100‑1 (MQ=255)
                                                                        ccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCa                        >  1:1352420/1‑100 (MQ=255)
                                                                         cgcgTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAg                       >  2:2308544/1‑100 (MQ=255)
                                                                          gcgTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGa                      >  1:614995/1‑100 (MQ=255)
                                                                              gAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGt                  <  1:2336295/100‑1 (MQ=255)
                                                                                aCGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTag                <  1:2221024/100‑1 (MQ=255)
                                                                                 cggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga               >  1:689988/1‑100 (MQ=255)
                                                                                   gcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaag             <  2:700921/100‑1 (MQ=255)
                                                                                    cggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaaga            >  2:616795/1‑100 (MQ=255)
                                                                                      gTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAgg          >  2:1495627/1‑100 (MQ=255)
                                                                                      gTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAgg          <  1:884230/100‑1 (MQ=255)
                                                                                       tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg         <  1:708008/100‑1 (MQ=255)
                                                                                       tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGGATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg         <  2:1646302/100‑1 (MQ=255)
                                                                                        aGATCTCATGAAAACGCTCACAGTCTGCGCCGACTGTTGGGGTAGAAATAACAAGCATCAGCTCATCAATATGATTGTTTACCAGACGGTAGAAGAGGGt        <  2:2405420/100‑1 (MQ=255)
                                                                                         gATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGtt       >  1:1588882/1‑100 (MQ=255)
                                                                                         gATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGtt       >  1:691255/1‑100 (MQ=255)
                                                                                         gATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGtt       >  1:368718/1‑100 (MQ=255)
                                                                                              cAGGAAAACGCTCACGGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt  <  1:2192742/100‑1 (MQ=255)
                                                                                              cAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt  >  1:1834341/1‑100 (MQ=255)
                                                                                              cAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt  >  1:1241697/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTT  >  NC_000913/1555227‑1555420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: