Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,557,343 C→T 16.3% T232T (ACG→ACA) ‡ ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,3430CT16.3% 206.6 / 26.9 91T232T (ACG→ACAddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (28/48);  new base T (8/7);  total (36/55)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

CGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAA  >  NC_000913/1557244‑1557442
                                                                                                   |                                                                                                   
gatctgATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGcc                                                                                                     <  1:477593/95‑1 (MQ=255)
 ggCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCg                                                                                                    <  1:238060/100‑1 (MQ=255)
  gCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGt                                                                                                   <  2:1352249/100‑1 (MQ=255)
   cAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTc                                                                                                  <  2:2354343/100‑1 (MQ=255)
    aGATCCGTTTTACGTAAGGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCa                                                                                                 <  2:1386775/100‑1 (MQ=255)
     gATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGccagatc                                                                                                >  2:477589/1‑95 (MQ=255)
     gATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg                                                                                                >  1:56883/1‑100 (MQ=255)
       tCCGTTTTACGTAATGCCCATTCTTCCTCCAGTGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCa                                                                                              <  2:1997982/100‑1 (MQ=255)
       tCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCa                                                                                              <  1:1105260/100‑1 (MQ=255)
        ccGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCAc                                                                                             <  2:807840/100‑1 (MQ=255)
          gTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACct                                                                                           <  1:202896/100‑1 (MQ=255)
            tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgc                                                                                         <  1:1706890/100‑1 (MQ=255)
            tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgc                                                                                         <  1:813193/100‑1 (MQ=255)
              tACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcac                                                                                       <  1:1833584/100‑3 (MQ=255)
               aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcaca                                                                                      <  1:774523/100‑4 (MQ=255)
               aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                      >  2:1269879/1‑100 (MQ=255)
                cGTAATGCCGATTCTTCCTCCAGCTGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacat                                                                                     <  2:549287/100‑5 (MQ=255)
                cGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacat                                                                                     <  1:2074039/100‑5 (MQ=255)
                 gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacatc                                                                                    <  2:1200897/100‑6 (MQ=38)
                 gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                      >  2:987368/1‑98 (MQ=255)
                 gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTgcgc                                                                                    >  1:719656/1‑100 (MQ=255)
                  tAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGcgcg                                                                                   <  2:1454111/100‑1 (MQ=255)
                     tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTc                                                                                 <  2:1811921/99‑1 (MQ=255)
                     tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCg                                                                                >  1:455471/1‑100 (MQ=255)
                      gctcgtgcttcttGCAGCTGTTTGTCAGTGGCTCGTGGGGTAGCCAGCAAGCATAGGGTGTATGGATGTGCATGTGCCGTCAGCACCTGCTTCtcgtcg                                                                                <  2:1347314/92‑1 (MQ=255)
                       cccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGCAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGcc                                                                              >  2:1027229/1‑100 (MQ=255)
                             cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                        <  2:1315389/100‑1 (MQ=255)
                             cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                        <  1:1468680/100‑1 (MQ=255)
                              ttgctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGGCGTCAGCACCTGTTGTTCGTCGCCCAGTTc                                                                        <  2:566507/96‑1 (MQ=255)
                              ttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                        <  2:269115/99‑1 (MQ=255)
                                cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTa                                                                      <  1:924012/99‑1 (MQ=255)
                                cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                     <  1:698082/100‑1 (MQ=255)
                                cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                     <  2:2005810/100‑1 (MQ=255)
                                   ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACa                                                                    <  2:1047306/98‑1 (MQ=255)
                                   ccAGCGGGTTATCAATGGCGGGGAGGGAATCCAGCAATAGTCAGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTGGCCCAGTTCTACAAt                                                                  >  2:1667160/1‑100 (MQ=255)
                                    cagcagTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc                                                                 <  1:205840/100‑1 (MQ=255)
                                    cAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc                                                                 >  2:31235/1‑100 (MQ=255)
                                      gCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTg                                                               >  1:780255/1‑100 (MQ=255)
                                        ggTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                             <  2:597794/100‑1 (MQ=255)
                                        ggTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                             >  2:1076397/1‑100 (MQ=255)
                                          tttATCAATGGCGGGGAGGGCATCCAGCAATACTCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                             >  2:925881/1‑98 (MQ=255)
                                          tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                           <  1:1514822/100‑1 (MQ=255)
                                          tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGAATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                           <  2:1219323/100‑1 (MQ=255)
                                            tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                         >  2:1794570/1‑100 (MQ=255)
                                            tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                         <  1:2380541/100‑1 (MQ=255)
                                              tCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGAt                                                       <  1:2098584/100‑1 (MQ=255)
                                                aaTGGCGGGGAGGGAATCCCGCAATAATTCTGCGGATGGGTGTGCAGGTGCCGGCAGCACCTGCCGCGCGTCGCCCAGTTCTGCACACTGCCCGGGacac                                                     >  2:2357726/1‑100 (MQ=255)
                                                aaTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATAc                                                     <  2:1455044/100‑1 (MQ=255)
                                                 aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                    >  1:1328764/1‑100 (MQ=255)
                                                 aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                    <  2:1094024/100‑1 (MQ=255)
                                                  tggtggGGAAGTAATCCAGCAATAATCGGGTGTATGTATGTGCAGGGGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                    <  2:961383/99‑1 (MQ=255)
                                                        ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                             >  2:219014/1‑100 (MQ=255)
                                                        ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                             <  2:40473/100‑1 (MQ=255)
                                                        ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                             >  1:2334623/1‑100 (MQ=255)
                                                         gagggaATCCCGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGcc                                            <  2:429455/100‑1 (MQ=255)
                                                          agggaATCCCGCAATAATGGGGTGTATGGATGTGCTGGTGCCGTCAGGACCTGCTGCGCTTCGCCCAGGTCTACAGTCTGCCCGAGATACATCACCGcc                                            <  2:616686/99‑1 (MQ=255)
                                                           gggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                          >  1:1375678/1‑100 (MQ=255)
                                                            ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                         <  2:1013609/100‑1 (MQ=255)
                                                             gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc                                        <  1:1412405/100‑1 (MQ=255)
                                                                 ccAGCAATAATCGGCTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATc                                    <  2:2493027/100‑1 (MQ=255)
                                                                    gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                 <  1:1593016/100‑1 (MQ=255)
                                                                    gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                 <  2:2524611/100‑1 (MQ=255)
                                                                     cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                <  2:1323603/100‑1 (MQ=255)
                                                                     cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                <  1:31236/100‑1 (MQ=255)
                                                                       ataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGGTCTACAAGCTGCCCGAGATACATCACCGCCACCCGATCGCTCat                              >  2:1120159/1‑100 (MQ=255)
                                                                        tcatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACACTCTGCCCGAGATACATCACCGCCACCCGATCGCTCatn                             <  2:1160435/98‑2 (MQ=255)
                                                                         aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCatat                            <  2:84804/100‑1 (MQ=255)
                                                                          atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATg                           <  1:1076405/100‑1 (MQ=255)
                                                                             gggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                        <  2:1472505/100‑1 (MQ=255)
                                                                              ggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc                         >  1:941001/1‑98 (MQ=255)
                                                                                tgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                     >  1:1601217/1‑100 (MQ=255)
                                                                                   aTGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc                  <  2:561953/100‑1 (MQ=255)
                                                                                    tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                 >  1:1426272/1‑100 (MQ=255)
                                                                                    tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                 >  2:2203342/1‑100 (MQ=255)
                                                                                       cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag              >  2:65671/5‑100 (MQ=38)
                                                                                       cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag              >  1:1854717/5‑100 (MQ=38)
                                                                                       cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag              >  1:718526/5‑100 (MQ=38)
                                                                                        tgtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga             >  1:2316644/1‑100 (MQ=255)
                                                                                        gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga             >  2:764402/4‑100 (MQ=38)
                                                                                        gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag              >  2:2367111/4‑99 (MQ=38)
                                                                                         gtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGAc            <  1:301582/100‑1 (MQ=255)
                                                                                         cagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGAc            >  1:1758165/3‑100 (MQ=39)
                                                                                          tgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACa           >  2:1382650/1‑100 (MQ=255)
                                                                                           gCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAt          >  1:240949/1‑100 (MQ=39)
                                                                                           gCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGACACAt          >  2:1051614/1‑100 (MQ=255)
                                                                                            cAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtt         >  2:1072636/1‑100 (MQ=255)
                                                                                             aGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCACATATGACGTATCACCGAGACATtg        >  2:74076/1‑100 (MQ=38)
                                                                                             aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg        <  2:493922/100‑1 (MQ=255)
                                                                                             aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCAATCGCTCATATGACGTATCACCGAGACa           >  2:458233/1‑97 (MQ=255)
                                                                                              ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtcg        >  2:1820790/1‑97 (MQ=255)
                                                                                                 gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg    <  1:818990/100‑1 (MQ=255)
                                                                                                 gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg    <  1:2251146/100‑1 (MQ=255)
                                                                                                 gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg    <  1:1520789/100‑1 (MQ=255)
                                                                                                   cGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCAAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaa  <  2:298438/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAA  >  NC_000913/1557244‑1557442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: