Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,557,345 T→A 16.5% T232S (ACG→TCG) ‡ ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,3450TA16.5% 206.5 / 34.3 91T232S (ACG→TCG) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (28/47);  new base A (8/7);  total (37/54)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-02

GCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAAT  >  NC_000913/1557246‑1557444
                                                                                                   |                                                                                                   
gCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGt                                                                                                     <  2:1352249/100‑1 (MQ=255)
 cAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTc                                                                                                    <  2:2354343/100‑1 (MQ=255)
  aGATCCGTTTTACGTAAGGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCa                                                                                                   <  2:1386775/100‑1 (MQ=255)
   gATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg                                                                                                  >  1:56883/1‑100 (MQ=255)
     tCCGTTTTACGTAATGCCCATTCTTCCTCCAGTGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCa                                                                                                <  2:1997982/100‑1 (MQ=255)
     tCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCa                                                                                                <  1:1105260/100‑1 (MQ=255)
      ccGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCAc                                                                                               <  2:807840/100‑1 (MQ=255)
        gTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACct                                                                                             <  1:202896/100‑1 (MQ=255)
          tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgc                                                                                           <  1:813193/100‑1 (MQ=255)
          tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgc                                                                                           <  1:1706890/100‑1 (MQ=255)
            tACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcac                                                                                         <  1:1833584/100‑3 (MQ=255)
             aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcaca                                                                                        <  1:774523/100‑4 (MQ=255)
             aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                        >  2:1269879/1‑100 (MQ=255)
              cGTAATGCCGATTCTTCCTCCAGCTGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacat                                                                                       <  2:549287/100‑5 (MQ=255)
              cGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacat                                                                                       <  1:2074039/100‑5 (MQ=255)
               gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacatc                                                                                      <  2:1200897/100‑6 (MQ=38)
               gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                        >  2:987368/1‑98 (MQ=255)
               gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTgcgc                                                                                      >  1:719656/1‑100 (MQ=255)
                tAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGcgcg                                                                                     <  2:1454111/100‑1 (MQ=255)
                   tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTc                                                                                   <  2:1811921/99‑1 (MQ=255)
                   tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCg                                                                                  >  1:455471/1‑100 (MQ=255)
                    gctcgtgcttcttGCAGCTGTTTGTCAGTGGCTCGTGGGGTAGCCAGCAAGCATAGGGTGTATGGATGTGCATGTGCCGTCAGCACCTGCTTCtcgtcg                                                                                  <  2:1347314/92‑1 (MQ=255)
                     cccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGCAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGcc                                                                                >  2:1027229/1‑100 (MQ=255)
                           cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                          <  1:1468680/100‑1 (MQ=255)
                           cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                          <  2:1315389/100‑1 (MQ=255)
                            ttgctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGGCGTCAGCACCTGTTGTTCGTCGCCCAGTTc                                                                          <  2:566507/96‑1 (MQ=255)
                            ttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                          <  2:269115/99‑1 (MQ=255)
                              cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTa                                                                        <  1:924012/99‑1 (MQ=255)
                              cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                       <  1:698082/100‑1 (MQ=255)
                              cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                       <  2:2005810/100‑1 (MQ=255)
                                 ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACa                                                                      <  2:1047306/98‑1 (MQ=255)
                                 ccAGCGGGTTATCAATGGCGGGGAGGGAATCCAGCAATAGTCAGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTGGCCCAGTTCTACAAt                                                                    >  2:1667160/1‑100 (MQ=255)
                                  cagcagTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc                                                                   <  1:205840/100‑1 (MQ=255)
                                  cAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc                                                                   >  2:31235/1‑100 (MQ=255)
                                    gCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTg                                                                 >  1:780255/1‑100 (MQ=255)
                                      ggTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                               <  2:597794/100‑1 (MQ=255)
                                      ggTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                               >  2:1076397/1‑100 (MQ=255)
                                        tttATCAATGGCGGGGAGGGCATCCAGCAATACTCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                               >  2:925881/1‑98 (MQ=255)
                                        tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                             <  1:1514822/100‑1 (MQ=255)
                                        tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGAATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                             <  2:1219323/100‑1 (MQ=255)
                                          tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                           >  2:1794570/1‑100 (MQ=255)
                                          tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                           <  1:2380541/100‑1 (MQ=255)
                                            tCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGAt                                                         <  1:2098584/100‑1 (MQ=255)
                                              aaTGGCGGGGAGGGAATCCCGCAATAATTCTGCGGATGGGTGTGCAGGTGCCGGCAGCACCTGCCGCGCGTCGCCCAGTTCTGCACACTGCCCGGGacac                                                       >  2:2357726/1‑100 (MQ=255)
                                              aaTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATAc                                                       <  2:1455044/100‑1 (MQ=255)
                                               aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                      <  2:1094024/100‑1 (MQ=255)
                                               aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                      >  1:1328764/1‑100 (MQ=255)
                                                tggtggGGAAGTAATCCAGCAATAATCGGGTGTATGTATGTGCAGGGGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                      <  2:961383/99‑1 (MQ=255)
                                                      ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                               >  2:219014/1‑100 (MQ=255)
                                                      ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                               <  2:40473/100‑1 (MQ=255)
                                                      ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                               >  1:2334623/1‑100 (MQ=255)
                                                       gagggaATCCCGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGcc                                              <  2:429455/100‑1 (MQ=255)
                                                        agggaATCCCGCAATAATGGGGTGTATGGATGTGCTGGTGCCGTCAGGACCTGCTGCGCTTCGCCCAGGTCTACAGTCTGCCCGAGATACATCACCGcc                                              <  2:616686/99‑1 (MQ=255)
                                                         gggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                            >  1:1375678/1‑100 (MQ=255)
                                                          ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                           <  2:1013609/100‑1 (MQ=255)
                                                           gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc                                          <  1:1412405/100‑1 (MQ=255)
                                                               ccAGCAATAATCGGCTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATc                                      <  2:2493027/100‑1 (MQ=255)
                                                                  gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                   <  1:1593016/100‑1 (MQ=255)
                                                                  gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                   <  2:2524611/100‑1 (MQ=255)
                                                                   cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                  <  1:31236/100‑1 (MQ=255)
                                                                   cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                  <  2:1323603/100‑1 (MQ=255)
                                                                     ataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGGTCTACAAGCTGCCCGAGATACATCACCGCCACCCGATCGCTCat                                >  2:1120159/1‑100 (MQ=255)
                                                                      tcatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACACTCTGCCCGAGATACATCACCGCCACCCGATCGCTCatn                               <  2:1160435/98‑2 (MQ=255)
                                                                       aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCatat                              <  2:84804/100‑1 (MQ=255)
                                                                        atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATg                             <  1:1076405/100‑1 (MQ=255)
                                                                           gggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                          <  2:1472505/100‑1 (MQ=255)
                                                                            ggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc                           >  1:941001/1‑98 (MQ=255)
                                                                              tgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                       >  1:1601217/1‑100 (MQ=255)
                                                                                 aTGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc                    <  2:561953/100‑1 (MQ=255)
                                                                                  tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                   >  1:1426272/1‑100 (MQ=255)
                                                                                  tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                   >  2:2203342/1‑100 (MQ=255)
                                                                                     cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                >  2:65671/5‑100 (MQ=38)
                                                                                     cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                >  1:1854717/5‑100 (MQ=38)
                                                                                     cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                >  1:718526/5‑100 (MQ=38)
                                                                                      tgtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga               >  1:2316644/1‑100 (MQ=255)
                                                                                      gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga               >  2:764402/4‑100 (MQ=38)
                                                                                      gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                >  2:2367111/4‑99 (MQ=38)
                                                                                       gtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGAc              <  1:301582/100‑1 (MQ=255)
                                                                                       cagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGAc              >  1:1758165/3‑100 (MQ=39)
                                                                                        tgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACa             >  2:1382650/1‑100 (MQ=255)
                                                                                         gCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAt            >  1:240949/1‑100 (MQ=39)
                                                                                         gCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGACACAt            >  2:1051614/1‑100 (MQ=255)
                                                                                          cAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtt           >  2:1072636/1‑100 (MQ=255)
                                                                                           aGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCACATATGACGTATCACCGAGACATtg          >  2:74076/1‑100 (MQ=38)
                                                                                           aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg          <  2:493922/100‑1 (MQ=255)
                                                                                           aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCAATCGCTCATATGACGTATCACCGAGACa             >  2:458233/1‑97 (MQ=255)
                                                                                            ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtcg          >  2:1820790/1‑97 (MQ=255)
                                                                                               gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg      <  1:2251146/100‑1 (MQ=255)
                                                                                               gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg      <  1:1520789/100‑1 (MQ=255)
                                                                                               gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg      <  1:818990/100‑1 (MQ=255)
                                                                                                 cGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCAAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaa    <  2:298438/100‑1 (MQ=255)
                                                                                                  gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGCCATTGGGTGaaa   >  2:587509/1‑100 (MQ=255)
                                                                                                   tCAGCACCTGCTGCGCGTCGCCCAGTGCTACAATCTGCCCGAGATACAACACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAAt  <  2:2269635/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAAT  >  NC_000913/1557246‑1557444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: