Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,557,347 A→G 16.5% L231P (CTG→CCG)  ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,3470AG16.5% 191.0 / 32.3 91L231P (CTG→CCG) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (29/47);  new base G (8/7);  total (37/54)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

AGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCA  >  NC_000913/1557248‑1557446
                                                                                                   |                                                                                                   
aGATCCGTTTTACGTAAGGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCa                                                                                                     <  2:1386775/100‑1 (MQ=255)
 gATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg                                                                                                    >  1:56883/1‑100 (MQ=255)
   tCCGTTTTACGTAATGCCCATTCTTCCTCCAGTGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCa                                                                                                  <  2:1997982/100‑1 (MQ=255)
   tCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCa                                                                                                  <  1:1105260/100‑1 (MQ=255)
    ccGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCAc                                                                                                 <  2:807840/100‑1 (MQ=255)
      gTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACct                                                                                               <  1:202896/100‑1 (MQ=255)
        tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgc                                                                                             <  1:813193/100‑1 (MQ=255)
        tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgc                                                                                             <  1:1706890/100‑1 (MQ=255)
          tACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcac                                                                                           <  1:1833584/100‑3 (MQ=255)
           aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcaca                                                                                          <  1:774523/100‑4 (MQ=255)
           aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                          >  2:1269879/1‑100 (MQ=255)
            cGTAATGCCGATTCTTCCTCCAGCTGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacat                                                                                         <  2:549287/100‑5 (MQ=255)
            cGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacat                                                                                         <  1:2074039/100‑5 (MQ=255)
             gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcacatc                                                                                        <  2:1200897/100‑6 (MQ=38)
             gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                          >  2:987368/1‑98 (MQ=255)
             gTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTgcgc                                                                                        >  1:719656/1‑100 (MQ=255)
              tAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGcgcg                                                                                       <  2:1454111/100‑1 (MQ=255)
                 tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTc                                                                                     <  2:1811921/99‑1 (MQ=255)
                 tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCg                                                                                    >  1:455471/1‑100 (MQ=255)
                  gctcgtgcttcttGCAGCTGTTTGTCAGTGGCTCGTGGGGTAGCCAGCAAGCATAGGGTGTATGGATGTGCATGTGCCGTCAGCACCTGCTTCtcgtcg                                                                                    <  2:1347314/92‑1 (MQ=255)
                   cccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGCAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGcc                                                                                  >  2:1027229/1‑100 (MQ=255)
                         cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                            <  1:1468680/100‑1 (MQ=255)
                         cttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                            <  2:1315389/100‑1 (MQ=255)
                          ttgctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGGCGTCAGCACCTGTTGTTCGTCGCCCAGTTc                                                                            <  2:566507/96‑1 (MQ=255)
                          ttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTc                                                                            <  2:269115/99‑1 (MQ=255)
                            cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTa                                                                          <  1:924012/99‑1 (MQ=255)
                            cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                         <  2:2005810/100‑1 (MQ=255)
                            cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                         <  1:698082/100‑1 (MQ=255)
                               ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACa                                                                        <  2:1047306/98‑1 (MQ=255)
                               ccAGCGGGTTATCAATGGCGGGGAGGGAATCCAGCAATAGTCAGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTGGCCCAGTTCTACAAt                                                                      >  2:1667160/1‑100 (MQ=255)
                                cagcagTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc                                                                     <  1:205840/100‑1 (MQ=255)
                                cAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATc                                                                     >  2:31235/1‑100 (MQ=255)
                                  gCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTg                                                                   >  1:780255/1‑100 (MQ=255)
                                    ggTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                                 <  2:597794/100‑1 (MQ=255)
                                    ggTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                                 >  2:1076397/1‑100 (MQ=255)
                                      tttATCAATGGCGGGGAGGGCATCCAGCAATACTCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                                 >  2:925881/1‑98 (MQ=255)
                                      tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                               <  1:1514822/100‑1 (MQ=255)
                                      tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGAATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                               <  2:1219323/100‑1 (MQ=255)
                                        tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                             >  2:1794570/1‑100 (MQ=255)
                                        tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                             <  1:2380541/100‑1 (MQ=255)
                                          tCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGAt                                                           <  1:2098584/100‑1 (MQ=255)
                                            aaTGGCGGGGAGGGAATCCCGCAATAATTCTGCGGATGGGTGTGCAGGTGCCGGCAGCACCTGCCGCGCGTCGCCCAGTTCTGCACACTGCCCGGGacac                                                         >  2:2357726/1‑100 (MQ=255)
                                            aaTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATAc                                                         <  2:1455044/100‑1 (MQ=255)
                                             aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                        <  2:1094024/100‑1 (MQ=255)
                                             aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                        >  1:1328764/1‑100 (MQ=255)
                                              tggtggGGAAGTAATCCAGCAATAATCGGGTGTATGTATGTGCAGGGGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                        <  2:961383/99‑1 (MQ=255)
                                                    ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                 >  2:219014/1‑100 (MQ=255)
                                                    ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                 >  1:2334623/1‑100 (MQ=255)
                                                    ggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                 <  2:40473/100‑1 (MQ=255)
                                                     gagggaATCCCGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGcc                                                <  2:429455/100‑1 (MQ=255)
                                                      agggaATCCCGCAATAATGGGGTGTATGGATGTGCTGGTGCCGTCAGGACCTGCTGCGCTTCGCCCAGGTCTACAGTCTGCCCGAGATACATCACCGcc                                                <  2:616686/99‑1 (MQ=255)
                                                       gggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                              >  1:1375678/1‑100 (MQ=255)
                                                        ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                             <  2:1013609/100‑1 (MQ=255)
                                                         gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAccc                                            <  1:1412405/100‑1 (MQ=255)
                                                             ccAGCAATAATCGGCTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATc                                        <  2:2493027/100‑1 (MQ=255)
                                                                gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                     <  1:1593016/100‑1 (MQ=255)
                                                                gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCt                                     <  2:2524611/100‑1 (MQ=255)
                                                                 cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                    <  2:1323603/100‑1 (MQ=255)
                                                                 cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                    <  1:31236/100‑1 (MQ=255)
                                                                   ataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGGTCTACAAGCTGCCCGAGATACATCACCGCCACCCGATCGCTCat                                  >  2:1120159/1‑100 (MQ=255)
                                                                    tcatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACACTCTGCCCGAGATACATCACCGCCACCCGATCGCTCatn                                 <  2:1160435/98‑2 (MQ=255)
                                                                     aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCatat                                <  2:84804/100‑1 (MQ=255)
                                                                      atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATg                               <  1:1076405/100‑1 (MQ=255)
                                                                         gggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                            <  2:1472505/100‑1 (MQ=255)
                                                                          ggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc                             >  1:941001/1‑98 (MQ=255)
                                                                            tgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                         >  1:1601217/1‑100 (MQ=255)
                                                                               aTGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc                      <  2:561953/100‑1 (MQ=255)
                                                                                tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                     >  1:1426272/1‑100 (MQ=255)
                                                                                tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                     >  2:2203342/1‑100 (MQ=255)
                                                                                   cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                  >  2:65671/5‑100 (MQ=38)
                                                                                   cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                  >  1:1854717/5‑100 (MQ=38)
                                                                                   cgcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                  >  1:718526/5‑100 (MQ=38)
                                                                                    tgtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga                 >  1:2316644/1‑100 (MQ=255)
                                                                                    gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgaga                 >  2:764402/4‑100 (MQ=38)
                                                                                    gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCgag                  >  2:2367111/4‑99 (MQ=38)
                                                                                     gtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGAc                <  1:301582/100‑1 (MQ=255)
                                                                                     cagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGAc                >  1:1758165/3‑100 (MQ=39)
                                                                                      tgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACa               >  2:1382650/1‑100 (MQ=255)
                                                                                       gCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAt              >  1:240949/1‑100 (MQ=39)
                                                                                       gCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGACACAt              >  2:1051614/1‑100 (MQ=255)
                                                                                        cAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtt             >  2:1072636/1‑100 (MQ=255)
                                                                                         aGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCACATATGACGTATCACCGAGACATtg            >  2:74076/1‑100 (MQ=38)
                                                                                         aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg            <  2:493922/100‑1 (MQ=255)
                                                                                         aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCAATCGCTCATATGACGTATCACCGAGACa               >  2:458233/1‑97 (MQ=255)
                                                                                          ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtcg            >  2:1820790/1‑97 (MQ=255)
                                                                                             gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg        <  1:2251146/100‑1 (MQ=255)
                                                                                             gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg        <  1:818990/100‑1 (MQ=255)
                                                                                             gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg        <  1:1520789/100‑1 (MQ=255)
                                                                                               cGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCAAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaa      <  2:298438/100‑1 (MQ=255)
                                                                                                gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGCCATTGGGTGaaa     >  2:587509/1‑100 (MQ=255)
                                                                                                 tCAGCACCTGCTGCGCGTCGCCCAGTGCTACAATCTGCCCGAGATACAACACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAAt    <  2:2269635/100‑1 (MQ=255)
                                                                                                  cAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTACCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGaaa     <  2:2429542/98‑1 (MQ=255)
                                                                                                   aGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCa  <  1:170395/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCA  >  NC_000913/1557248‑1557446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: